IN VITRO EXPANSION OF HUMAN PB CD 34 ' CELLS 3601 MATERIALS AND METHODS Blood samples

To elucidate the role of recombinant human colony-stimulating factors (CSFs) for expanding peripheral blood (PB) CD34' cells, these cells were purified up to 94.5% f 1.3% and the effects of individual and combined CSFs on the proliferation and differentiation of these cells were studied in a 7-day suspension culture. The majority of CD34+ cells coexpressed CD38 (81.8% f 5.1 %), but was negative for CD33 (88.5% f 3.4%). Among the individual CSFs examined, recombinant interleukin-3 (rlL-3) was identified as the most potent factor for expanding PB progenitor cells and increased nonerythroid progenitor cells 13f 4-fold (P < .01). Recombinant granulocyte-macrophage colony-stimulating factor (rGM-CSF), recombinant granulocyte-CSF (rG-CSF), recombinant macrophage-CSF (rM-CSF), rlL-6, rlL-I 1, and recombinant stem cell factor (rSCF) did not alone expand nonerythroid progenitor cells.

[1]  M. Yamaguchi,et al.  Proliferation and differentiation of myelodysplastic CD34+ cells in serum‐free medium: response to individual colony‐stimulating factors , 1993, British journal of haematology.

[2]  R. Hoffman,et al.  Long-term generation and expansion of human primitive hematopoietic progenitor cells in vitro. , 1993, Blood.

[3]  L. To,et al.  Ex vivo expansion and maturation of peripheral blood CD34+ cells into the myeloid lineage. , 1992, Blood.

[4]  A. Hirao,et al.  Combination of recombinant cytokines fails to produce ex vivo expansion of human blood hematopoietic progenitor cells , 1992, Annals of Hematology.

[5]  M. Clarke,et al.  In vitro myelopoiesis stimulated by rapid medium exchange and supplementation with hematopoietic growth factors. , 1991, Blood.

[6]  C. Dai,et al.  Transitional change of colony stimulating factor requirements for erythroid progenitors , 1991, Journal of cellular physiology.

[7]  K. Sawada,et al.  Purification of human marrow progenitor cells and demonstration of the direct action of macrophage colony-stimulating factor on colony-forming unit-macrophage. , 1991, Blood.

[8]  S. Emerson,et al.  Rapid medium perfusion rate significantly increases the productivity and longevity of human bone marrow cultures. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[9]  J. Armitage,et al.  High-dose cyclophosphamide, carmustine, and etoposide followed by autologous peripheral stem cell transplantation for patients with relapsed Hodgkin's disease. , 1991, Blood.

[10]  I. Bernstein,et al.  Recombinant human stem cell factor enhances the formation of colonies by CD34+ and CD34+lin- cells, and the generation of colony-forming cell progeny from CD34+lin- cells cultured with interleukin-3, granulocyte colony-stimulating factor, or granulocyte-macrophage colony-stimulating factor. , 1991, Blood.

[11]  M. Moore,et al.  Purification and partial characterization of a human hematopoietic precursor population. , 1991, Blood.

[12]  B. Palsson,et al.  Culture perfusion schedules influence the metabolic activity and granulocyte‐macrophage colony‐stimulating factor production rates of human bone marrow stromal cells , 1991, Journal of cellular physiology.

[13]  P. Lansdorp,et al.  Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34+CD38- progenitor cells. , 1991, Blood.

[14]  P. Lansdorp,et al.  Flow cytometry for clinical estimation of circulating hematopoietic progenitors for autologous transplantation in cancer patients. , 1991, Blood.

[15]  K. Zsebo,et al.  Identification, purification, and biological characterization of hematopoietic stem cell factor from buffalo rat liver-conditioned medium , 1990, Cell.

[16]  C. Verfaillie,et al.  Purified primitive human hematopoietic progenitor cells with long-term in vitro repopulating capacity adhere selectively to irradiated bone marrow stroma , 1990, The Journal of experimental medicine.

[17]  S. Koury,et al.  Purification of human blood burst‐forming units‐erythroid and demonstration of the evolution of erythropoietin receptors , 1990, Journal of cellular physiology.

[18]  E. Barker,et al.  Peripheral blood-derived stem cell collections for use in autologous transplantation after high dose chemotherapy: an alternative approach. , 1990, Bone marrow transplantation.

[19]  D. Weisenburger,et al.  Allogeneic transplantation of blood-derived, T cell-depleted hemopoietic stem cells after myeloablative treatment in a patient with acute lymphoblastic leukemia. , 1989, Bone marrow transplantation.

[20]  A. Pileri,et al.  GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR TO HARVEST CIRCULATING HAEMOPOIETIC STEM CELLS FOR AUTOTRANSPLANTATION , 1989, The Lancet.

[21]  S. Koury,et al.  Human colony-forming units-erythroid do not require accessory cells, but do require direct interaction with insulin-like growth factor I and/or insulin for erythroid development. , 1989, The Journal of clinical investigation.

[22]  J. Ihle,et al.  Synergistic factors for stem cell proliferation: further studies of the target stem cells and the mechanism of stimulation by interleukin-1, interleukin-6, and granulocyte colony-stimulating factor. , 1988, Blood.

[23]  J. Villeval,et al.  Effects of recombinant human granulocyte colony-stimulating factor on hematopoietic progenitor cells in cancer patients. , 1988, Blood.

[24]  M. Ogawa,et al.  Synergism between interleukin-6 and interleukin-3 in supporting proliferation of human hematopoietic stem cells: comparison with interleukin-1 alpha. , 1988, Blood.

[25]  D. Weisenburger,et al.  Autologous peripheral hematopoietic stem cell transplantation restores hematopoietic function following marrow ablative therapy. , 1988, Blood.

[26]  L. To,et al.  CFU-mix are no better than CFU-GM in predicting hemopoietic reconstitutive capacity of peripheral blood stem cells collected in the very early remission phase of acute nonlymphoblastic leukemia. , 1987, Experimental hematology.

[27]  D. Oscier,et al.  Circulating stem cell autografts. , 1986, Bone marrow transplantation.

[28]  P. Montcuquet,et al.  Autologous bone marrow transplantation (ABMT) for acute leukaemia in complete remission: a pilot study of 33 cases , 1986, British journal of haematology.

[29]  G. Marit,et al.  Successful autologous transplantation with peripheral blood hemopoietic cells in a patient with acute leukemia. , 1986, Experimental hematology.

[30]  L. To,et al.  THE PERIPHERAL BLOOD CFU‐mix: CFU‐GM RATIO DURING VERY EARLY REMISSION FROM ACUTE NON‐LYMPHOBLASTIC LEUKAEMIA , 1986, British journal of haematology.

[31]  B. Dörken,et al.  Autologous transplantation of blood-derived hemopoietic stem cells after myeloablative therapy in a patient with Burkitt's lymphoma. , 1986, Blood.

[32]  L. To,et al.  Circulating autologous stem cells collected in very early remission from acute non‐lymphoblastic leukaemia produce prompt but incomplete haemopoietic reconstitution after high dose melphalan or supralethal chemoradiotherapy , 1985, British journal of haematology.

[33]  L. To,et al.  High levels of circulating haemopoietic stem cells in very early remission from acute non‐lymphoblastic leukaemia and their collection and cryopreservation , 1984, British journal of haematology.

[34]  D. Housman,et al.  Human erythroid burst-forming unit: T-cell requirement for proliferation in vitro , 1978, The Journal of experimental medicine.

[35]  K. Zsebo,et al.  Recombinant rat stem cell factor stimulates the amplification and differentiation of fractionated mouse stem cell populations. , 1992, Blood.

[36]  R. Porat,et al.  Restoration of in vitro hematopoiesis in B-chronic lymphocytic leukemia by antibodies to tumor necrosis factor. , 1991, Leukemia research.

[37]  J. Armitage,et al.  Cryopreservation and infusion of autologous peripheral blood stem cells. , 1990, Bone marrow transplantation.

[38]  Y. Lévy,et al.  Treatment of aggressive multiple myeloma by high-dose chemotherapy and total body irradiation followed by blood stem cells autologous graft. , 1989, Blood.

[39]  C. Caux,et al.  Sequential loss of CD34 and class II MHC antigens on purified cord blood hematopoietic progenitors cultured with IL-3: characterization of CD34-, HLA-DR+ cells. , 1989, Blood.

[40]  D. Weisenburger,et al.  High-dose therapy and autologous peripheral blood stem cell transplantation for patients with lymphoma. , 1989, Blood.

[41]  J. Adamson,et al.  Interleukin 1 stimulates fibroblasts to synthesize granulocyte-macrophage and granulocyte colony-stimulating factors. Mechanism for the hematopoietic response to inflammation. , 1988, The Journal of clinical investigation.