Isolation of aerobic bacterial agents from the lungs of sheep and goats with pneumonia and detection of Pasteurella multocida and Mannheimia haemolytica by polymerase chain reaction.

The purpose of this study was to isolate Pasteurella spp and other aerobic bacterial agents from the lungs of sheep and goats with pneumonia and to identify Pasteurella multocida and Mannheimia haemolytica by both culture methods and polymerase chain reaction (PCR). In addition, mouse pathogenecity tests were carried out on suspected P. multocida isolates. In the examination of lung samples collected from sheep, 15 (4.3%) P. multocida and 8 (2.3%) M. haemolytica strains were isolated and identified. The numbers of species identified in the goat samples were 1 (0.7%) for P. multocida and 6 (4%) for M. haemolytica. The differences between the numbers of P. multocida and M. haemolytica strains isolated from the sheep and goat lung samples were not statistically significant (P = 0.2 in sheep and P = 0.12 in goats). Thirteen (86.7%) P. multocida isolates were positive in the mouse pathogenecity test in sheep. One P. multocida isolate from goats was also positive in the mouse pathogenecity test. All P. multocida and M. haemolytica strains that tested positive by culture also tested positive by PCR. However, no toxigenic P. multocida were detected in any isolates by PCR using primers derived from the toxA gene. In conclusion, this study showed the feasibilty of PCR for the accurate and rapid identification of P. multocida and M. haemolytica.

[1]  S. Highlander,et al.  Molecular genetic analysis of virulence in Mannheimia (pasteurella) haemolytica. , 2001, Frontiers in bioscience : a journal and virtual library.

[2]  M. Raji,et al.  The effects of climatic factors on the occurrence and gross pathological lesions in bacterial pneumonia of ovine and caprine hosts in Zaria, Nigeria. , 2001 .

[3]  O. Kaya,et al.  Evaluation of Immunogenicity of Pasterurella haemolytica Serotypes in Experimental Models , 2000 .

[4]  M. Bisgaard,et al.  Investigations on the carrier rate of Pasteurella multocida in healthy commercial poultry flocks and flocks affected by fowl cholera , 2000, Avian pathology : journal of the W.V.P.A.

[5]  I. Wilkie,et al.  PCR detection and analysis of Pasteurella multocida from the tonsils of slaughtered pigs in Vietnam. , 2000, Veterinary microbiology.

[6]  K. Brogden,et al.  Pasteurella haemolytica complicated respiratory infections in sheep and goats. , 1998, Veterinary research.

[7]  J. Papadimitriou,et al.  Development of PCR Assays for Species- and Type-Specific Identification of Pasteurella multocidaIsolates , 1998, Journal of Clinical Microbiology.

[8]  T. Carpenter,et al.  Detection of Pasteurella multocida-specific DNA in turkey flocks by use of the polymerase chain reaction. , 1997, Avian diseases.

[9]  H. Hotzel,et al.  [Detection of dermonecrotic toxin genes in Pasteurella multocida strains using the polymerase chain reaction (PCR)]. , 1997, Berliner und Munchener tierarztliche Wochenschrift.

[10]  E. Vimr,et al.  Direct PCR analysis for toxigenic Pasteurella multocida , 1996, Journal of clinical microbiology.

[11]  M. Smits,et al.  A Specific and Sensitive PCR Assay Suitable for Large-Scale Detection of Toxigenic Pasteurella Multocida in Nasal and Tonsillar Swabs Specimens of Pigs , 1996, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[12]  J. Varga,et al.  Coagglutination test for serotyping Pasteurella haemolytica , 1996, Journal of clinical microbiology.

[13]  R. Kasten,et al.  Pasteurella multocida produces a protein with homology to the P6 outer membrane protein of Haemophilus influenzae , 1995, Infection and immunity.

[14]  R. Haziroğlu,et al.  Studies of the pathology and microbiology of pneumonic lungs of lambs. , 1994, DTW. Deutsche tierarztliche Wochenschrift.

[15]  S. Nagai,et al.  Differentiation of toxigenic from nontoxigenic isolates of Pasteurella multocida by PCR , 1994, Journal of clinical microbiology.

[16]  H. Ball,et al.  Pasteurella haemolytica serotypes isolated in Northern Ireland during 1989-1991. , 1993, The British veterinary journal.

[17]  N. Foged.  Pasteurella multocida toxin. The characterisation of the toxin and its significance in the diagnosis and prevention of progressive atrophic rhinitis in pigs. , 1992, APMIS. Supplementum.

[18]  L. C. Fallavena,et al.  Pneumonic pasteurellosis due to P multocida in a flock of lambs in Brazil , 1991, Veterinary Record.

[19]  D. Davies.  Aetiology of pneumonias of young sheep. , 1985, Progress in veterinary microbiology and immunology.

[20]  K. Kume,et al.  Characterization of dermonecrotic toxin produced by serotype D strains of Pasteurella multocida. , 1984, American journal of veterinary research.

[21]  W. Yates.  A review of infectious bovine rhinotracheitis, shipping fever pneumonia and viral-bacterial synergism in respiratory disease of cattle. , 1982, Canadian journal of comparative medicine : Revue canadienne de medecine comparee.

[22]  T. George.  Investigations of respiratory disease of sheep in Australia. , 1972, Australian veterinary journal.