Splicing regulators: targets and drugs

Silencing of splicing regulators by RNA interference, combined with splicing-specific microarrays, has revealed a complex network of distinct alternative splicing events in Drosophila, while a high-throughput screen of more than 6,000 compounds has identified drugs that interfere specifically and directly with one class of splicing regulators in human cells.

[1]  A. Krainer,et al.  Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins. , 2001, Molecular cell.

[2]  M. Garcia-Blanco.  Messenger RNA reprogramming by spliceosome-mediated RNA trans-splicing. , 2003, The Journal of clinical investigation.

[3]  T. Cooper,et al.  Pre-mRNA splicing and human disease. , 2003, Genes & development.

[4]  B. Graveley.  Sorting out the complexity of SR protein functions. , 2000, RNA.

[5]  A. Krainer,et al.  Regulation of alternative splicing in vivo by overexpression of antagonistic splicing factors. , 1994, Science.

[6]  J. Castle,et al.  Genome-Wide Survey of Human Alternative Pre-mRNA Splicing with Exon Junction Microarrays , 2003, Science.

[7]  Eric E Schadt,et al.  Optimization of oligonucleotide arrays and RNA amplification protocols for analysis of transcript structure and alternative splicing , 2003, Genome Biology.

[8]  Tyson A. Clark,et al.  Genomewide Analysis of mRNA Processing in Yeast Using Splicing-Specific Microarrays , 2002, Science.

[9]  K. Musunuru.  Cell-specific RNA-binding proteins in human disease. , 2003, Trends in cardiovascular medicine.

[10]  M. Olive,et al.  hnRNP A1 Recruited to an Exon In Vivo Can Function as an Exon Splicing Silencer , 1999, Molecular and Cellular Biology.

[11]  S. Brenner,et al.  Global analysis of positive and negative pre-mRNA splicing regulators in Drosophila. , 2005, Genes & development.

[12]  Christopher J. Lee,et al.  Genome-wide detection of alternative splicing in expressed sequences of human genes , 2001, Nucleic Acids Res..

[13]  M. Manoharan,et al.  Modification of splicing in the dystrophin gene in cultured Mdx muscle cells by antisense oligoribonucleotides. , 1998, Human molecular genetics.

[14]  B. Frey,et al.  Revealing global regulatory features of mammalian alternative splicing using a quantitative microarray platform. , 2004, Molecular cell.

[15]  M. Gabut,et al.  Selective modification of alternative splicing by indole derivatives that target serine-arginine-rich protein splicing factors. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[16]  A. Krainer,et al.  Listening to silence and understanding nonsense: exonic mutations that affect splicing , 2002, Nature Reviews Genetics.

[17]  F. Muntoni,et al.  Bifunctional antisense oligonucleotides provide a trans-acting splicing enhancer that stimulates SMN2 gene expression in patient fibroblasts , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[18]  T. Cooper,et al.  Finding signals that regulate alternative splicing in the post-genomic era , 2002, Genome Biology.

[19]  A. Kornblihtt,et al.  Alternative splicing: multiple control mechanisms and involvement in human disease. , 2002, Trends in genetics : TIG.

[20]  Zhi-Ming Zheng,et al.  Regulation of Alternative RNA Splicing by Exon Definition and Exon Sequences in Viral and Mammalian Gene Expression , 2004, Journal of Biomedical Science.

[21]  Brenton R Graveley,et al.  Identification of alternative splicing regulators by RNA interference in Drosophila. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[22]  M. Garcia-Blanco,et al.  Alternative splicing in disease and therapy , 2004, Nature Biotechnology.

[23]  A. Krainer,et al.  Correction of disease-associated exon skipping by synthetic exon-specific activators , 2003, Nature Structural Biology.

[24]  International Human Genome Sequencing Consortium.  Initial sequencing and analysis of the human genome , 2001, Nature.