Detection of phenobarbital in bloodstains, semen, seminal stains, saliva, saliva stains, perspiration stains, and hair.

Low nanogram quantities of phenobarbital were detected in a 10-μL dried blood stain and in other physiological fluids by using radioimmunoassay. The age of the stain versus detectability of phenobarbital and the cross-reactivity of vaginal secretions were investigated.