What causes wing wear in foraging bumble bees?

SUMMARY Flying is an ecologically important behaviour in many insects, but it often results in permanent wing damage. Although wing wear in insects is often used as a method to determine insect age, and is associated with an increased risk of mortality, the causes of wing wear are unresolved. In this paper, we examine whether wing use while foraging explains wing wear in bumble bees (Bombus spp.). Wing wear may result from three distinct flight characteristics during foraging: time spent in flight, flight frequency and frequency of wing collisions with vegetation. To test these hypotheses for causes of wing wear, we recorded digital video of individually marked bumble bees foraging in nature on 12 different plant species that result in variation in these flight characteristics, and recaptured these individuals to photograph their wings over time. Bumble bees with a higher frequency of wing collisions showed an increased loss of wing area, which became more severe over time. Neither time in flight nor flight frequency was uniquely and significantly associated with wing wear. Therefore, the collision frequency hypothesis best explained wing wear in bumble bees. We conclude that wing use during foraging in bumble bees results in wing wear. Wing wear reflects behaviour, not simply age. Because wing wear has elsewhere been shown to increase mortality, this study provides an important mechanism linking foraging behaviour with lifespan.