Presence and Diversity of Extended-Spectrum Cephalosporin Resistance Among Escherichia coli from Urban Wastewater and Feedlot Cattle, in Alberta, Canada.

A recent preliminary study from our group found that extended-spectrum cephalosporin-resistance determinants can be detected in the majority of composite fecal samples collected from Alberta feedlot cattle. Most notably, blaCTX-M genes were detected in 46.5% of samples. Further isolate characterization identified blaCTX-M-15 and blaCTX-M-27, which are widespread in bacteria from humans. We hypothesized that Escherichia coli of human and beef cattle origins share the same pool of blaCTX-M genes. In this study, we aimed to assess and compare the genomic profiles of a larger collection of blaCTX-M-positive E. coli recovered from fecal composite samples from Canadian beef feedlot cattle and human wastewater through whole-genome sequencing. The variants blaCTX-M-55, blaCTX-M-32, blaCTX-M-27, blaCTX-M-15, and blaCTX-M-14 were found in both urban wastewater and cattle fecal isolates. Core genome multilocus sequence typing showed little similarity between the fecal and wastewater isolates. Thus, if the dissemination of genes between urban wastewater and feedlot cattle occurs, it does not appear to be related to the expansion of specific clonal lineages. Further investigations are warranted to assemble and compare plasmids carrying these genes to better understand the modalities and directionality of transfer.

[1]  T. Smieszek,et al.  Selection and co-selection of antibiotic resistances among Escherichia coli by antibiotic use in primary care: An ecological analysis , 2019, bioRxiv.

[2]  C. Carrillo,et al.  Selection of Multidrug-Resistant Bacteria in Medicated Animal Feeds , 2019, Front. Microbiol..

[3]  J. Madec,et al.  Emergence of blaCTX-M-55 associated with fosA, rmtB and mcr gene variants in Escherichia coli from various animal species in France , 2018, The Journal of antimicrobial chemotherapy.

[4]  K. Gilbride,et al.  A closer look at the antibiotic‐resistant bacterial community found in urban wastewater treatment systems , 2018, MicrobiologyOpen.

[5]  Yang Wang,et al.  Occurrence and characterisation of ESBL-encoding plasmids among Escherichia coli isolates from fresh vegetables. , 2018, Veterinary microbiology.

[6]  P. Ruegg,et al.  Characteristics and genetic diversity of multi-drug resistant extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolated from bovine mastitis , 2017, Oncotarget.

[7]  G. Côté,et al.  Determinants of virulence and of resistance to ceftiofur, gentamicin, and spectinomycin in clinical Escherichia coli from broiler chickens in Québec, Canada. , 2017, Veterinary microbiology.

[8]  J. Morris,et al.  Identification and Characterization of Cefotaxime Resistant Bacteria in Beef Cattle , 2016, PloS one.

[9]  L. N. Andrade,et al.  Evaluation and characterization of plasmids carrying CTX-M genes in a non-clonal population of multidrug-resistant Enterobacteriaceae isolated from poultry in Brazil. , 2016, Diagnostic microbiology and infectious disease.

[10]  Anke Huss,et al.  Human–livestock contacts and their relationship to transmission of zoonotic pathogens, a systematic review of literature , 2016, One Health.

[11]  Shaohua Zhao,et al.  WGS accurately predicts antimicrobial resistance in Escherichia coli. , 2015, The Journal of antimicrobial chemotherapy.

[12]  Jian-Hua Liu,et al.  F33: A-: B-, IncHI2/ST3, and IncI1/ST71 plasmids drive the dissemination of fosA3 and blaCTX−M−55/−14/−65 in Escherichia coli from chickens in China , 2014, Front. Microbiol..

[13]  G. Koraimann,et al.  Characterization and comparative analysis of antibiotic resistance plasmids isolated from a wastewater treatment plant , 2014, Front. Microbiol..

[14]  B. Norby,et al.  Effects of Ceftiofur and Chlortetracycline Treatment Strategies on Antimicrobial Susceptibility and on tet(A), tet(B), and bla CMY-2 Resistance Genes among E. coli Isolated from the Feces of Feedlot Cattle , 2013, PloS one.

[15]  J. R. Johnson,et al.  Predicting antimicrobial susceptibilities for Escherichia coli and Klebsiella pneumoniae isolates using whole genomic sequence data , 2013, The Journal of antimicrobial chemotherapy.

[16]  N. Woodford,et al.  Multiresistant Gram-negative bacteria: the role of high-risk clones in the dissemination of antibiotic resistance. , 2011, FEMS microbiology reviews.

[17]  F. Aarestrup,et al.  Evaluation of eight different cephalosporins for detection of cephalosporin resistance in Salmonella enterica and Escherichia coli. , 2010, Microbial drug resistance.

[18]  D. Deforce,et al.  Complete Nucleotide Sequence of CTX-M-15-Plasmids from Clinical Escherichia coli Isolates: Insertional Events of Transposons and Insertion Sequences , 2010, PloS one.

[19]  L. Wieler,et al.  Emergence of human pandemic O25:H4-ST131 CTX-M-15 extended-spectrum-beta-lactamase-producing Escherichia coli among companion animals. , 2010, The Journal of antimicrobial chemotherapy.

[20]  F. Aarestrup,et al.  beta-Lactamases among extended-spectrum beta-lactamase (ESBL)-resistant Salmonella from poultry, poultry products and human patients in The Netherlands. , 2005, The Journal of antimicrobial chemotherapy.

[21]  W. Laegreid,et al.  Correlation of enterohemorrhagic Escherichia coli O157 prevalence in feces, hides, and carcasses of beef cattle during processing. , 2000, Proceedings of the National Academy of Sciences of the United States of America.