Cascaded multiple amplification strategy for ultrasensitive detection of HIV/HCV virus DNA.

Ultrasensitive detection of HIV and HCV virus DNA is of great importance for early accurate diagnostics and therapy of HIV virus-infected patients. Herein, to our best knowledge, it is the first to use DNA cascaded multiple amplification strategy for ultrasensitive detection of HIV virus DNA with G-quadruplex-specific fluorescent or colorimetric probes as signal carriers. The developed strategy also exhibited universal applicability for HCV virus DNA detection. After reaction for about 4h, high sensitivity and specificity can be achieved at both fluorescent and colorimetric strategies (limit of detection (LOD) of 10 fM and 0.5pM were reached for fluorescent and colorimetric detection, respectively). And the single-based mismatched DNA even can be distinguished by naked eyes. It is believed that the cascaded multiple amplification strategy presents a huge advance in sensing platform and potential application in future clinical diagnosis.

[1]  Yaqing Liu,et al.  Integration of graphene oxide and DNA as a universal platform for multiple arithmetic logic units. , 2014, Chemical communications.

[2]  Tao Liu,et al.  Label-free, isothermal and ultrasensitive electrochemical detection of DNA and DNA 3'-phosphatase using a cascade enzymatic cleavage strategy. , 2015, Chemical communications.

[3]  Kun Wang,et al.  Reconfigurable and resettable arithmetic logic units based on magnetic beads and DNA. , 2015, Nanoscale.

[4]  Chunhai Fan,et al.  Lab in a tube: ultrasensitive detection of microRNAs at the single-cell level and in breast cancer patients using quadratic isothermal amplification. , 2013, Journal of the American Chemical Society.

[5]  K. Mullis,et al.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. , 1988, Science.

[6]  David L. Thomas,et al.  The challenge of hepatitis C in the HIV-infected person. , 2008, Annual review of medicine.

[7]  R. Service,et al.  Coming Soon: The Pocket DNA Sequencer , 1998, Science.

[8]  C. Fan,et al.  Target-triggered three-way junction structure and polymerase/nicking enzyme synergetic isothermal quadratic DNA machine for highly specific, one-step, and rapid microRNA detection at attomolar level. , 2014, Analytical chemistry.

[9]  Kun Wang,et al.  Highly efficient colorimetric detection of ATP utilizing a split aptamer target binding strategy and superior catalytic activity of graphene oxide–platinum/gold nanoparticles , 2015 .

[10]  Y. Liaw,et al.  Constitutive activation of nuclear factor κB in hepatocellular carcinoma , 2000 .

[11]  E. Wang,et al.  Highly sensitive and specific colorimetric detection of cancer cells via dual-aptamer target binding strategy. , 2015, Biosensors & bioelectronics.

[12]  G A Satten,et al.  New testing strategy to detect early HIV-1 infection for use in incidence estimates and for clinical and prevention purposes. , 1998, JAMA.

[13]  Ai-Li Sun,et al.  Exonuclease III-based target recycling for ultrasensitive homogeneous monitoring of HIV DNA using Ag(+)-coordinated hairpin probe. , 2015, Biosensors & bioelectronics.

[14]  J. Berzofsky,et al.  Cell-mediated immune response to human immunodeficiency virus (HIV) type 1 in seronegative homosexual men with recent sexual exposure to HIV-1. , 1992, The Journal of infectious diseases.

[15]  I. Willner,et al.  Amplified and multiplexed detection of DNA using the dendritic rolling circle amplified synthesis of DNAzyme reporter units. , 2014, Analytical chemistry.

[16]  K. Sherman,et al.  HIV/hepatitis C coinfection natural history and disease progression. , 2011, Current opinion in HIV and AIDS.

[17]  Ronghua Yang,et al.  Aptamer degradation inhibition combined with DNAzyme cascade-based signal amplification for colorimetric detection of proteins. , 2013, Chemical communications.

[18]  Bradley Duncan,et al.  Bacteriophage-based nanoprobes for rapid bacteria separation. , 2015, Nanoscale.

[19]  Ye Teng,et al.  A label-free fluorescent molecular beacon based on DNA-Ag nanoclusters for the construction of versatile Biosensors. , 2015, Biosensors & bioelectronics.

[20]  Jiangtao Ren,et al.  Application of DNA machine in amplified DNA detection. , 2014, Chemical communications.

[21]  Tao Li,et al.  G-quadruplex-based DNAzyme for sensitive mercury detection with the naked eye. , 2009, Chemical communications.

[22]  Y. Liaw,et al.  Constitutive activation of nuclear factor kappaB in hepatocellular carcinoma. , 2000, Cancer.

[23]  Tao Li,et al.  G-quadruplex aptamers with peroxidase-like DNAzyme functions: which is the best and how does it work? , 2009, Chemistry, an Asian journal.

[24]  M. Sulkowski,et al.  Management of patients coinfected with HCV and HIV: a close look at the role for direct-acting antivirals. , 2012, Gastroenterology.

[25]  W. Tan,et al.  Hybridization-triggered isothermal signal amplification coupled with MutS for label-free and sensitive fluorescent assay of SNPs. , 2012, Chemical communications.

[26]  Guonan Chen,et al.  A label-free electrochemical biosensor for detection of HIV related gene based on interaction between DNA and protein , 2013 .

[27]  R. Karlsson,et al.  Kinetic analysis of monoclonal antibody-antigen interactions with a new biosensor based analytical system. , 1991, Journal of immunological methods.

[28]  Yun Xiang,et al.  Quadratic recycling amplification for label-free and sensitive visual detection of HIV DNA. , 2014, Biosensors & bioelectronics.

[29]  Sai Bi,et al.  Triggered polycatenated DNA scaffolds for DNA sensors and aptasensors by a combination of rolling circle amplification and DNAzyme amplification. , 2010, Analytical chemistry.