An Integrated Device for Monitoring Time‐Dependent in vitro Expression From Single Genes in Picolitre Droplets
暂无分享,去创建一个
Daniel Bratton | Wilhelm T S Huck | Fabienne Courtois | Graeme Whyte | Florian Hollfelder | Chris Abell | Fabienne Courtois | C. Abell | F. Hollfelder | W. Huck | G. Whyte | D. Bratton | Luis F Olguin | Luis F. Olguín | L. Olguín
[1] Andrew D Griffiths,et al. High-throughput screens and selections of enzyme-encoding genes. , 2005, Current opinion in chemical biology.
[2] Helen Song,et al. Reactions in droplets in microfluidic channels. , 2006, Angewandte Chemie.
[3] F. Ghadessy,et al. A novel emulsion mixture for in vitro compartmentalization of transcription and translation in the rabbit reticulocyte system. , 2004, Protein engineering, design & selection : PEDS.
[4] Dan S. Tawfik,et al. High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments. , 2005, Chemistry & biology.
[5] Petra Schwille,et al. A New Embedded Process for Compartmentalized Cell‐Free Protein Expression and On‐line Detection in Microfluidic Devices , 2005, Chembiochem : a European journal of chemical biology.
[6] D. Weitz,et al. Geometrically mediated breakup of drops in microfluidic devices. , 2003, Physical review letters.
[7] H. Stone,et al. Formation of dispersions using “flow focusing” in microchannels , 2003 .
[8] A. deMello,et al. Quantitative detection of protein expression in single cells using droplet microfluidics. , 2007, Chemical communications.
[9] Dan S. Tawfik,et al. Man-made enzymes--from design to in vitro compartmentalisation. , 2000, Current opinion in biotechnology.
[10] M. L. Simpson,et al. Nano-enabled synthetic biology , 2007, Molecular systems biology.
[11] M. Kanouni,et al. Preparation of a stable double emulsion (W1/O/W2): role of the interfacial films on the stability of the system. , 2002, Advances in colloid and interface science.
[12] Tetsuya Yomo,et al. Femtoliter compartment in liposomes for in vitro selection of proteins. , 2006, Analytical biochemistry.
[13] Andrew D Griffiths,et al. Directed evolution by in vitro compartmentalization , 2006, Nature Methods.
[14] Rustem F Ismagilov,et al. A microfluidic approach for screening submicroliter volumes against multiple reagents by using preformed arrays of nanoliter plugs in a three-phase liquid/liquid/gas flow. , 2005, Angewandte Chemie.
[15] Dan S. Tawfik,et al. Man-made cell-like compartments for molecular evolution , 1998, Nature Biotechnology.
[16] Delai L Chen,et al. Microgram-scale testing of reaction conditions in solution using nanoliter plugs in microfluidics with detection by MALDI-MS. , 2006, Journal of the American Chemical Society.
[17] Piotr Garstecki,et al. Bifurcation of droplet flows within capillaries. , 2006, Physical review. E, Statistical, nonlinear, and soft matter physics.
[18] Vincent Noireaux,et al. A vesicle bioreactor as a step toward an artificial cell assembly. , 2004, Proceedings of the National Academy of Sciences of the United States of America.
[19] A. Griffiths,et al. Droplets as Microreactors for High‐Throughput Biology , 2007, Chembiochem : a European journal of chemical biology.
[20] Liang Li,et al. Using three-phase flow of immiscible liquids to prevent coalescence of droplets in microfluidic channels: criteria to identify the third liquid and validation with protein crystallization. , 2007, Langmuir : the ACS journal of surfaces and colloids.
[21] Wilhelm T S Huck,et al. Surface-induced droplet fusion in microfluidic devices. , 2007, Lab on a chip.
[22] Rustem F Ismagilov,et al. Microfluidic cartridges preloaded with nanoliter plugs of reagents: an alternative to 96-well plates for screening. , 2006, Current opinion in chemical biology.
[23] R. Bar-Ziv,et al. Principles of cell-free genetic circuit assembly , 2003, Proceedings of the National Academy of Sciences of the United States of America.
[24] Frank Diehl,et al. BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions , 2006, Nature Methods.
[25] A. Griffiths,et al. High-throughput screening of enzyme libraries: in vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions. , 2005, Chemistry & biology.
[26] R. Westervelt,et al. Dielectrophoretic manipulation of drops for high-speed microfluidic sorting devices , 2006 .
[27] David A. Weitz,et al. Mixing characterization inside microdroplets engineered on a microcoalescer , 2007 .
[28] Joshua D. Tice,et al. Microfluidic systems for chemical kinetics that rely on chaotic mixing in droplets , 2004, Philosophical Transactions of the Royal Society of London. Series A: Mathematical, Physical and Engineering Sciences.
[29] Andrew D Griffiths,et al. Miniaturising the laboratory in emulsion droplets. , 2006, Trends in biotechnology.
[30] Jérôme Bibette,et al. Emulsions: basic principles , 1999 .
[31] D. Weitz,et al. Electric control of droplets in microfluidic devices. , 2006, Angewandte Chemie.
[32] K. Johnsson,et al. The laboratory in a droplet. , 2005, Chemistry & biology.
[33] P. Gröbner,et al. Use of T7 RNA polymerase in an optimized Escherichia coli coupled in vitro transcription-translation system. Application in regulatory studies and expression of long transcription units. , 1996, European journal of biochemistry.
[34] G. Whitesides,et al. Solvent compatibility of poly(dimethylsiloxane)-based microfluidic devices. , 2003, Analytical chemistry.
[35] Hans Lehrach,et al. Cell-free protein expression and functional assay in nanowell chip format. , 2004, Analytical chemistry.
[36] David A. Weitz,et al. Electrocoalescence of drops synchronized by size-dependent flow in microfluidic channels , 2006 .
[37] Dan S. Tawfik,et al. In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting. , 2004, Analytical biochemistry.
[38] Reliable quantification of in vitro synthesized green fluorescent protein: Comparison of fluorescence activity and total protein levels , 2001, Electrophoresis.
[39] Helen Song,et al. Millisecond kinetics on a microfluidic chip using nanoliters of reagents. , 2003, Journal of the American Chemical Society.
[40] Helen Song,et al. On-chip titration of an anticoagulant argatroban and determination of the clotting time within whole blood or plasma using a plug-based microfluidic system. , 2006, Analytical chemistry.
[41] Andrew D Griffiths,et al. Miniaturizing chemistry and biology in microdroplets. , 2007, Chemical communications.
[42] Helen Song,et al. Reaktionen in Mikrofluidiktröpfchen , 2006 .
[43] T. Egelhoff,et al. Green fluorescent protein and epitope tag fusion vectors for Dictyostelium discoideum. , 2000, Plasmid.