Genomic in situ hybridization (GISH) of Tripsacum dactyloides and Zea mays ssp. mays with B chromosomes

Genomic affinities between Tripsacum dactyloides (2n = 72) and Zea mays ssp. mays (2n = 20 + 5 B) were analyzed through GISH (genomic in situ hybridization) to ascertain the degree of chromosome homology between the two genera. Mitotic cells of T. dactyloides were simultaneously probed with total genomic DNA from Z. mays ssp .mays (2n = 20) and with rDNA (pTA71). A disperse pattern of hybridization signal among all 72 chromosomes, corresponding to maize total DNA, and six strong fluorescent signals due to the rDNA probe hybridizing on 3 chromosome pairs of T. dactyloides were observed. Mitotic chromosomes from Z. mays ssp. mays (2n = 20 + 5 B) were hybridized with a maize line that lacked B chromosomes and knobs and with total DNA from T. dactyloides. The knobless line of maize hybridized intensely on all chromosomes except for some regions where the probe bound less. Tripsacum dactyloides bound intensely on one terminal region of each B chromosome and to some regions of chromosome pairs 2, 6, and 8. Thes...

[1]  L. Poggio,et al.  Genome size and numerical polymorphism for the B chromosome in races of maize (Zea mays ssp. mays, Poaceae). , 1998, American journal of botany.

[2]  J. Cohen.  Corn Genome Pops Out of the Pack , 1997, Science.

[3]  J. Bennetzen,et al.  The unified grass genome: synergy in synteny. , 1997, Genome research.

[4]  B. Kindiger,et al.  Assignment of a gene(s) conferring apomixis in Tripsacum to a chromosome arm: cytological and molecular evidence. , 1996, Genome.

[5]  L. Poggio,et al.  Genetic control of B chromosome transmission rate in Zea mays ssp. mays (Poaceae) , 1996 .

[6]  R. N. Jones,et al.  B chromosomes in plants. , 1995, The New phytologist.

[7]  N. Jouve,et al.  Fluorescent in situ hybridization and C-banding analyses of highly repetitive DNA sequences in the heterochromatin of rye (Secale montanum Guss.) and wheat incorporating S. montanum chromosome segments. , 1995, Genome.

[8]  G. Moore,et al.  Cereal Genome Evolution: Grasses, line up and form a circle , 1995, Current Biology.

[9]  N. Jouve,et al.  Highly repetitive sequences in B chromosomes of Secale cereale revealed by fluorescence in situ hybridization. , 1994, Genome.

[10]  B. Kindiger,et al.  Cytological evidence supporting a procedure for directing and enhancing pairing between maize and Tripsacum , 1990 .

[11]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[12]  W. Gerlach,et al.  Cloning and characterization of ribosomal RNA genes from wheat and barley. , 1979, Nucleic acids research.

[13]  W. R. Carlson.  The B chromosome of corn. , 1978, Annual review of genetics.

[14]  J. Harlan,et al.  Pathways of genetic transfer from Tripsacum to Zea mays. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[15]  M. Maguire.  DIVERGENCE IN TRIPSACUM AND ZEA CHROMOSOMES , 1961 .

[16]  R. G. Reeves,et al.  HYBRIDIZATION OF MAIZE, TRIPSACUM, AND EUCHLAENA , 1931 .