O-glycoligases, a new category of glycoside bond-forming mutant glycosidases, catalyse facile syntheses of isoprimeverosides.

A mutant α-xylosidase that lacks its catalytic acid/base residue efficiently catalyses the synthesis of O-linked isoprimeverosides (Xyl-α-1,6GlcOR) in near quantitative yields.

[1]  M. Rossi,et al.  beta-Glycosyl azides as substrates for alpha-glycosynthases: preparation of efficient alpha-L-fucosynthases. , 2009, Chemistry & biology.

[2]  Soichi Wakatsuki,et al.  1,2‐α‐l‐Fucosynthase: A glycosynthase derived from an inverting α‐glycosidase with an unusual reaction mechanism , 2008, FEBS letters.

[3]  M. Okuyama,et al.  Aglycone specificity of Escherichia coliα‐xylosidase investigated by transxylosylation , 2007, The FEBS journal.

[4]  Young-Wan Kim,et al.  Expanding the thioglycoligase strategy to the synthesis of alpha-linked thioglycosides allows structural investigation of the parent enzyme/substrate complex. , 2006, Journal of the American Chemical Society.

[5]  S. Withers,et al.  Mechanistic and Structural Analysis of a Family 31 α-Glycosidase and Its Glycosyl-enzyme Intermediate* , 2005, Journal of Biological Chemistry.

[6]  S. Withers,et al.  An unusual mechanism of glycoside hydrolysis involving redox and elimination steps by a family 4 beta-glycosidase from Thermotoga maritima. , 2004, Journal of the American Chemical Society.

[7]  S. Withers,et al.  Thioglycoligases: mutant glycosidases for thioglycoside synthesis. , 2003, Angewandte Chemie.

[8]  M. Okuyama,et al.  α-Glucosidase Mutant Catalyzes “α-Glycosynthase”-type Reaction , 2002 .

[9]  M. Rossi,et al.  Enzymatic synthesis and hydrolysis of xylogluco-oligosaccharides using the first archaeal α-xylosidase from Sulfolobus solfataricus , 2001, Extremophiles.

[10]  S. Withers,et al.  β-Mannosynthase: Synthesis of β-Mannosides with a Mutant β-Mannosidase. , 2001, Angewandte Chemie.

[11]  S. Withers,et al.  Glycosidase mechanisms: anatomy of a finely tuned catalyst. , 1999, Accounts of chemical research.

[12]  S. Withers,et al.  Glycosynthases: Mutant Glycosidases for Oligosaccharide Synthesis , 1998 .

[13]  S. Withers,et al.  Engineering of a thioglycoligase: randomized mutagenesis of the acid-base residue leads to the identification of improved catalysts. , 2005, Protein engineering, design & selection : PEDS.