A fluorescence lifetime imaging scanning confocal endomicroscope

We describe a fluorescence lifetime imaging endomicroscope employing a fibre bundle probe and time correlated single photon counting. Preliminary images of stained pollen grains, eGFP-labelled cells exhibiting Förster resonant energy transfer and tissue autofluorescence are presented.

[1]  M A A Neil,et al.  Toward the clinical application of time-domain fluorescence lifetime imaging. , 2005, Journal of biomedical optics.

[2]  F. Wouters,et al.  Fluorescence lifetime imaging of receptor tyrosine kinase activity in cells , 1999, Current Biology.

[3]  B. Viellerobe,et al.  Fibered confocal spectroscopy and multicolor imaging system for in vivo fluorescence analysis. , 2007, Optics express.

[4]  N. Tamai,et al.  Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy. , 2007, Analytical chemistry.

[5]  Remo Guidieri.  Res , 1995, RES: Anthropology and Aesthetics.

[6]  A. Visser,et al.  Time-resolved fluorescence depolarization of rhodamine B and octadecylrhodamine B in Triton X-100 micelles and Aerosol OT reversed micelles. , 1988 .

[7]  M. Neil,et al.  A compact, multidimensional spectrofluorometer exploiting supercontinuum generation , 2008, Journal of biophotonics.

[8]  H. van den Bergh,et al.  Frequency-domain fluorescence lifetime imaging for endoscopic clinical cancer photodetection: Apparatus design and preliminary results , 1997, Journal of Fluorescence.

[9]  A. Floren,et al.  ' " ' " ' " . " ' " " " " " ' " ' " " " " " : ' " 1 , 2001 .

[10]  D. Tank,et al.  A Miniature Head-Mounted Two-Photon Microscope High-Resolution Brain Imaging in Freely Moving Animals , 2001, Neuron.

[11]  R Richards-Kortum,et al.  Fiber confocal reflectance microscope (FCRM) for in-vivo imaging. , 2001, Optics express.

[12]  A. Gmitro,et al.  Confocal microscopy through a fiber-optic imaging bundle. , 1993, Optics letters.

[13]  Kenneth P. Ghiggino,et al.  Fluorescence lifetime measurements using a novel fiber‐optic laser scanning confocal microscope , 1992 .

[14]  G. Kino,et al.  Silicon-micromachined scanning confocal optical microscope , 1998 .

[15]  E. Vicaut,et al.  Fibered Confocal Fluorescence Microscopy (Cell-viZio™) Facilitates Extended Imaging in the Field of Microcirculation , 2004, Journal of Vascular Research.

[16]  F Lacombe,et al.  Coherent femtosecond pulse shaping for the optimization of a non-linear micro-endoscope. , 2007, Optics express.

[17]  Hans C. Gerritsen,et al.  High frame rate fluorescence lifetime imaging , 2003 .

[18]  Jens Eickhoff,et al.  In vivo multiphoton fluorescence lifetime imaging of protein-bound and free nicotinamide adenine dinucleotide in normal and precancerous epithelia. , 2007, Journal of biomedical optics.

[19]  A. Rouse,et al.  Multispectral imaging with a confocal microendoscope. , 2000, Optics letters.