Low Intraspecific Diversity Suggesting Genetic Drift in Gloriosa superba L. (Liliaceae) in Konkan Coast of Karnataka, India

The population of Gloriosa species is gradually diminishing in Udupi district of Karnataka, due to their over exploitation for colchicine-the therapeutic alkaloid. The scattered and scant occurrence of this genus is further attributed to their seasonal and truncated seed set in nature. The present study is the first report on polymorphism studies in this plant using AFLP, the dominant molecular marker. Genetic distance based on the AFLP data could authenticate, low intraspecific diversity more efficiently than RAPD. This genetic similarity identified could be correlated with collection sites and altitude. 2C DNA values of the collected accessions ranged between 5.24 and 5.81 pg, when determined by flow cytometer. The study indicated that the genetically isolated smaller population of G. superba is experiencing genetic drift within the Udupi district of Karnataka, India and hence must be subjected to suitable conservation measures.

[1]  B. Ghosh,et al.  Polymorphism in Gloriosa superba , 2009, Plant Genetic Resources.

[2]  Jing Ma,et al.  Identification of molecular markers in soybean comparing RFLP, RAPD and AFLP DNA mapping techniques , 1996, Plant Molecular Biology Reporter.

[3]  天野 淳二,et al.  Early Identification of Intra- and Intergeneric Hybrids among Colchicaceous Ornamentals, Gloriosa spp., Littonia modesta Hook. and Sandersonia aurantiaca Hook., by Flow Cytometry and Random Amplified Polymorphic DNA Analyses , 2007 .

[4]  E. Nevo,et al.  Ecological correlates of RAPD DNA diversity of wild barley, Hordeum spontaneum, in the Fertile Crescent , 1998, Genetic Resources and Crop Evolution.

[5]  L. Gustafsson,et al.  Low genetic variation in Swedish populations of the rare speciesVicia pisiformis (Fabaceae) revealed with rflp (rDNA) and RAPD , 2004, Plant Systematics and Evolution.

[6]  J. Stewart,et al.  Genetic diversity in and phylogenetic relationships of the Brazilian endemic cotton,Gossypium mustelinum (Malvaceae) , 1994, Plant Systematics and Evolution.

[7]  P. Laird,et al.  Global and gene-specific epigenetic patterns in human bladder cancer genomes are relatively stable in vivo and in vitro over time. , 2001, Cancer research.

[8]  R. von Bothmer,et al.  Genetic variation and differentiation in Nordic populations of Elymus alaskanus (Scrib. ex Merr.) Löve (Poaceae) , 1999, Theoretical and Applied Genetics.

[9]  M. Geber,et al.  Variation among populations of Clarkia Unguiculata (Onagraceae) along altitudinal and latitudinal gradients. , 1999, American journal of botany.

[10]  O. Panaud,et al.  Genome size and base composition of seven Quercus species: inter- and intra-population variation , 1998 .

[11]  M. Koch,et al.  Molecular biogeography and evolution of the Microthlaspi perfoliatum s.l. polyploid complex (Brassicaceae): chloroplast DNA and nuclear ribosomal DNA restriction site variation , 1998 .

[12]  F. González-Candelas,et al.  Lack of genetic variability in the rare and endangered Limonium cavanillesii (Plumbaginaceae) using RAPD markers , 1997 .

[13]  F. Yeh.  Population genetic analysis of codominant and dominant markers and quantitative traits. , 1997 .

[14]  L. Rieseberg,et al.  Conservation Genetics of Endangered Island Plants , 1996 .

[15]  A. Simpson,et al.  Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. , 1994, BioTechniques.

[16]  M. Lynch,et al.  Analysis of population genetic structure with RAPD markers , 1994, Molecular ecology.

[17]  D. Galbraith,et al.  Nuclear DNA content of monocotyledons and related taxa , 1994 .

[18]  J. Hamrick,et al.  GENETIC DIVERSITY AND POPULATION STRUCTURE IN TRADESCANTIA HIRSUTICAULIS (COMMELINACEAE) , 1993 .

[19]  B. Schierwater,et al.  Applications of random amplified polymorphic DNA (RAPD) in molecular ecology , 1992, Molecular ecology.

[20]  K. Holsinger,et al.  Correlations between species traits and allozyme diversity: implications for conservation biology. , 1991 .

[21]  D. Govindaraju.  Variation in gene flow levels among predominantly self‐pollinated plants , 1989 .

[22]  D. Waller,et al.  Genetic variation in the extreme endemic Pedicularis furbishiae (Scrophulariaceae) , 1987 .

[23]  C. Cullis,et al.  Genome Organization and Variation in Higher Plants , 1987 .

[24]  E. Pahlich,et al.  A rapid DNA isolation procedure for small quantities of fresh leaf tissue , 1980 .

[25]  D. Wilken.  Local Differentiation for Phenotypic Plasticity in the Annual Collomia linearis (Polemoniaceae) , 1977 .

[26]  Masatoshi Nei,et al.  Genetic Distance between Populations , 1972, The American Naturalist.

[27]  F. C. Vasek,et al.  Seasonal Progression of Flowering in Clarkia , 1971 .

[28]  F. C. Vasek.  THE EVOLUTION OF CLARKIA UNGUICULATA DERIVATIVES ADAPTED TO RELATIVELY XERIC ENVIRONMENTS , 1964 .