In‐vivo and in‐vitro anti‐inflammatory effect of Echinacea purpurea and Hypericum perforatum

Echinacea purpurea (L.) Moench and Hypericum perforatum (L.) were evaluated for their anti‐inflammatory activity against carrageenan‐induced paw oedema in mice. Each drug was administered orally to mice at 30 and 100 mg kg−1, twice daily. Only the higher dose significantly inhibited, time dependently, the formation of oedema, evaluated as area under the curve (echinacea P < 0.01; hypericum P < 0.05). Western blot analysis showed that in‐vivo treatment with these extracts could modulate lipopolysaccharide (LPS) and interferon‐γ induced cyclooxygenase‐2 (COX‐2) and inducible nitric oxide synthase (iNOS) expression in peritoneal macrophages. In particular, treatment with 100 mg kg−1 hypericum inhibited both iNOS and COX‐2 expression, whereas treatment with 100 mg kg−1 echinacea down‐regulated only COX‐2 expression. The present study suggests that the anti‐inflammatory effect of these extracts could be in part related to their modulation of COX‐2 expression.

[1]  M. Schäfer,et al.  Efficacy of Echinacea purpurea in Patients with a Common Cold , 2001, Arzneimittelforschung.

[2]  E. Elovic,et al.  St. John's Wort , 2001, The Journal of head trauma rehabilitation.

[3]  R. T. Coutts,et al.  Comparison of chemical components and antioxidant capacity of different Echinacea species , 2001, The Journal of pharmacy and pharmacology.

[4]  E. Ernst,et al.  St John's wort: Prozac from the plant kingdom. , 2001, Trends in pharmacological sciences.

[5]  J. Phillipson,et al.  St John's wort (Hypericum perforatum L.): a review of its chemistry, pharmacology and clinical properties , 2001, The Journal of pharmacy and pharmacology.

[6]  Prof Vikas Kumar,et al.  Anti-inflammatory and analgesic activity of Indian Hypericum perforatum L. , 2001, Indian journal of experimental biology.

[7]  R. Meli,et al.  Inhibition of inducible nitric oxide synthase and cyclooxygenase-2 expression by flavonoids in macrophage J774A.1. , 2001, Life sciences.

[8]  S. Percival,et al.  Use of echinacea in medicine. , 2000, Biochemical pharmacology.

[9]  S. Kareh.  Toxicology and Clinical Pharmacology of Herbal Products , 2000, Forensic Science and Medicine.

[10]  M. Pšenák,et al.  Effect of dianthrones and their precursors from Hypericum perforatum L. on lipoxygenase activity. , 1999, Die Pharmazie.

[11]  M. Schmitz,et al.  Hypericin as a non-antioxidant inhibitor of NF-κB , 1999 .

[12]  M. Schmitz,et al.  Hypericin as a non-antioxidant inhibitor of NF-kappa B. , 1999, Planta medica.

[13]  A. Ianaro,et al.  Interaction between nitric oxide and cyclooxygenase pathways. , 1996, Prostaglandins, leukotrienes, and essential fatty acids.

[14]  P. Agostinis,et al.  Photosensitized inhibition of growth factor-regulated protein kinases by hypericin. , 1995, Biochemical pharmacology.

[15]  R. Bauer,et al.  In Vitro Inhibition of Cyclooxygenase and 5-Lipoxygenase by Alkamides from Echinacea and Achillea Species , 1994, Planta medica.

[16]  A. Nussler,et al.  Inflammation, immunoregulation, and inducible nitric oxide synthase , 1993, Journal of leukocyte biology.

[17]  M. Lohmann‐Matthes,et al.  Polysaccharides isolated from plant cell cultures of Echinacea purpurea enhance the resistance of immunosuppressed mice against systemic infections with Candida albicans and Listeria monocytogenes. , 1993, International journal of immunopharmacology.

[18]  S. Hart,et al.  Selective expression of mitogen-inducible cyclooxygenase in macrophages stimulated with lipopolysaccharide. , 1992, The Journal of biological chemistry.

[19]  U. Mengs,et al.  Toxicity of Echinacea purpurea. Acute, subacute and genotoxicity studies. , 1991, Arzneimittel-Forschung.

[20]  M. Lohmann‐Matthes,et al.  Application of purified polysaccharides from cell cultures of the plant Echinacea purpurea to test subjects mediates activation of the phagocyte system. , 1991, International journal of immunopharmacology.

[21]  S. Okpanyi,et al.  [Genotoxicity of a standardized Hypericum extract]. , 1990, Arzneimittel-Forschung.

[22]  T. Schleich,et al.  [The effect of Echinacea purpurea Moench on phagocytosis in granulocytes measured by chemiluminescence]. , 1990, Arzneimittel-Forschung.

[23]  A. Tubaro,et al.  Anti-inflammatory activity of Echinacea angustifolia fractions separated on the basis of molecular weight. , 1988, Pharmacological research communications.

[24]  M. Zenk,et al.  Immunologically active polysaccharides of Echinacea purpurea cell cultures , 1988 .

[25]  Paolo Negro,et al.  Anti‐inflammatory activity of a polysaccharidic fraction of Echinacea angustifolia , 1987, The Journal of pharmacy and pharmacology.

[26]  M. Martins,et al.  Mouse paw edema. A new model for inflammation? , 1987, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas.

[27]  M. Lohmann‐Matthes,et al.  Macrophage activation and induction of macrophage cytotoxicity by purified polysaccharide fractions from the plant Echinacea purpurea , 1984, Infection and immunity.

[28]  S. Amagaya,et al.  Anti-inflammatory testing methods: comparative evaluation of mice and rats. , 1981, Journal of pharmacobio-dynamics.