[Study on the localization of neocarzinostatin in various tissues by enzyme-labeled antibody method--with special reference to the distribution into oral region (author's transl)].

The antitumor antibiotic, Neocarzinostatin (NCS) was isolated by Ishida et al. from the culture filtrates of Streptomyces carzinostaticus F-41. The antitumor activities of NCS have been demonstrated against various mouse and rat ascitic and solid tumors and clinical applications of NCS have been shown to be effective against acute leukemia, stomach cancer, pancreatic cancer , bladder cancer and oral cancer. The purpose of this study is to examine the localization of NCS in oral tissues by means of enzyme-labeled antibody method. The experimental methods were as follows, anti-NCS antibody was made by immunization with NCS against rabbits and anti-NCS IgG was prepared by DEAE cellulose column chromatography. Labeling of horseradish peroxidase to IgG and preparation of tissue specimens were followed by Nakane's method. The findings were as follows; (1) In oral region, NCS was distributed highly in squamous cell epithelium of oral cavity and it had a tendency to be affinitive to gland tissues of salivary glands and it was detected highly and sebaceous glands, too. (2) The distribution of the drug was little in lymphatic tissues such as the spleen and the thymus but it was detected highly in gland tissues of the gastro-intestinal tract and the pancreas. In the kidney, NCS was detected very highly in renal tubules. (3) Against ascitic Sarcoma 180 cells, NCS adheared to the membranes of tumor cells within 5 minutes after injection and disappeared after 4 hours.