Short‐term control of glucokinase activity: role of a regulatory protein

Glucokinase is one of the four hexokinases present in mammalian tissues. It is expressed in two cell types that have to respond to changes in the blood glucose concentration, the liver parenchymal cell and the β‐cells of pancreatic islets. The former are responsible for the metabolism and storage of an important part of the ingested glucose, whereas the latter secrete insulin in response to an increase in the blood glucose level. One major characteristic of glucokinase is that it has a relatively low affinity for glucose and displays positive cooperativity for this substrate, despite the fact that it is a monometic enzyme. Furthermore, unlike other hexokinases, it is not inhibited by micromolar (physiological) concentrations of glucose 6‐phosphate but by a regulatory protein that transduces the effect of fructose 6‐phosphate and of fructose 1‐phosphate. The purpose of this review is to describe these aspects of the regulation of glucokinase.— Van Schaftingen, E., Detheux, M., Veiga da Cunha, M. Short‐term control of glucokinase activity; role of a regulatory protein. FASEB J. 8: 414‐419; 1994.

[1]  J. Vandekerckhove,et al.  Cloning and sequencing of rat liver cDNAs encoding the regulatory protein of glucokinase , 1993, FEBS letters.

[2]  E. Van Schaftingen,et al.  Species and tissue distribution of the regulatory protein of glucokinase. , 1993, The Biochemical journal.

[3]  P. Iynedjian.  Mammalian glucokinase and its gene. , 1993, The Biochemical journal.

[4]  J. Guinovart,et al.  Stimulation of glucose utilization by fructose in isolated rat hepatocytes. , 1993, Archives of biochemistry and biophysics.

[5]  M. Permutt,et al.  Glucokinase and NIDDM: A Candidate Gene That Paid Off , 1992, Diabetes.

[6]  E. Van Schaftingen,et al.  Binding of sorbitol 6-phosphate and of fructose 1-phosphate to the regulatory protein of liver glucokinase. , 1992, The Biochemical journal.

[7]  E. Van Schaftingen,et al.  Mechanism of the stimulatory effect of a potassium-rich medium on the phosphorylation of glucose in isolated rat hepatocytes. , 1992, European journal of biochemistry.

[8]  M. Magnuson.  Tissue‐Specific regulation of glucokinase gene expression , 1992, Journal of cellular biochemistry.

[9]  E. Van Schaftingen,et al.  Competitive inhibition of liver glucokinase by its regulatory protein. , 1991, European journal of biochemistry.

[10]  E. Van Schaftingen,et al.  Effectors of the regulatory protein acting on liver glucokinase: a kinetic investigation. , 1991, European journal of biochemistry.

[11]  E. Van Schaftingen,et al.  Fructose administration stimulates glucose phosphorylation in the livers of anesthetized rats , 1991, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[12]  E. Van Schaftingen,et al.  Fructose 1-phosphate and the regulation of glucokinase activity in isolated hepatocytes. , 1990, European journal of biochemistry.

[13]  E. Van Schaftingen,et al.  The mechanism by which rat liver glucokinase is inhibited by the regulatory protein. , 1990, European journal of biochemistry.

[14]  K. Neet,et al.  Demonstration of a slow conformational change in liver glucokinase by fluorescence spectroscopy. , 1990, The Journal of biological chemistry.

[15]  E. Van Schaftingen,et al.  Regulation of glucokinase by a fructose-1-phosphate-sensitive protein in pancreatic islets. , 1990, European journal of biochemistry.

[16]  F M Matschinsky,et al.  Glucokinase as Glucose Sensor and Metabolic Signal Generator in Pancreatic β-Cells and Hepatocytes , 1990, Diabetes.

[17]  R. Middleton.  Hexokinases and glucokinases. , 1990, Biochemical Society transactions.

[18]  E. Van Schaftingen,et al.  Stimulation of glucose phosphorylation by fructose in isolated rat hepatocytes. , 1990, European journal of biochemistry.

[19]  E. Van Schaftingen.  A protein from rat liver confers to glucokinase the property of being antagonistically regulated by fructose 6-phosphate and fructose 1-phosphate. , 1989, European journal of biochemistry.

[20]  H. Lodish,et al.  Cloning and functional expression in bacteria of a novel glucose transporter present in liver, intestine, kidney, and β-pancreatic islet cells , 1988, Cell.

[21]  M. Rider,et al.  Palmitate inhibits liver glycolysis. Involvement of fructose 2,6-bisphosphate in the glucose/fatty acid cycle. , 1988, The Biochemical journal.

[22]  J. Mikkelsen,et al.  Acyl-CoA-binding protein from cow. Binding characteristics and cellular and tissue distribution. , 1987, The Biochemical journal.

[23]  Kinetics of hexokinase D ('glucokinase') with inosine triphosphate as phosphate donor. Loss of kinetic co-operativity with respect to glucose. , 1987, The Biochemical journal.

[24]  A. Cornish-Bowden,et al.  Co-operativity in monomeric enzymes. , 1987, Journal of theoretical biology.

[25]  A. Cornish-Bowden,et al.  Mechanistic origin of the sigmoidal rate behaviour of rat liver hexokinase D ('glucokinase'). , 1986, The Biochemical journal.

[26]  G. van den Berghe.  Fructose: metabolism and short-term effects on carbohydrate and purine metabolic pathways. , 1986, Progress in biochemical pharmacology.

[27]  F. Matschinsky,et al.  Pancreatic islet glucose metabolism and regulation of insulin secretion. , 1986, Diabetes/metabolism reviews.

[28]  M. Cárdenas,et al.  Suppression of kinetic cooperativity of hexokinase D (glucokinase) by competitive inhibitors. A slow transition model. , 1984, European journal of biochemistry.

[29]  H G Hers,et al.  Gluconeogenesis and related aspects of glycolysis. , 1983, Annual review of biochemistry.

[30]  D. Topping,et al.  Effects of fructose concentration on carbohydrate metabolism, heat production and substrate cycling in isolated rat hepatocytes. , 1979, The Biochemical journal.

[31]  F. Bontemps,et al.  Phosphorylation of glucose in isolated rat hepatocytes. Sigmoidal kinetics explained by the activity of glucokinase alone. , 1978, Biochemical Journal.

[32]  S. Weinhouse,et al.  Regulation of glucokinase in liver. , 1976, Current topics in cellular regulation.

[33]  P. Wals,et al.  Recycling of glucose by rat hepatocytes. , 1975, European journal of biochemistry.

[34]  C. Hales,et al.  The inhibition of rat liver glucokinase by palmitoyl-CoA. , 1969, Biochimica et biophysica acta.