Localization of P2X1 purinoceptors by autoradiography and immunohistochemistry in rat kidneys.

P2 receptors have been identified in rat kidney by autoradiography, using the radioligand [3H]α,β-methylene ATP, and by immunohistochemistry, using a polyclonal antibody to the P2X1 purinoceptor. They have been localized to the vascular smooth muscle of intrarenal arteries, including arcuate and interlobular arteries, and afferent arterioles, but not glomeruli, postglomerular efferent arterioles, or renal tubules. We conclude that at least some of the P2 receptors present on vascular smooth muscle are of the P2X1 subtype. The functional significance of these findings in the vascular control of the kidney is discussed.

[1]  P. Churchill,et al.  Purinergic P2y receptors stimulate renin secretion by rat renal cortical slices. , 1993, The Journal of pharmacology and experimental therapeutics.

[2]  G. Buell,et al.  P2X Receptors: An Emerging Channel Family , 1996, The European journal of neuroscience.

[3]  R. Berne.  Cardiac nucleotides in hypoxia: possible role in regulation of coronary blood flow. , 1963, The American journal of physiology.

[4]  N. Harboe,et al.  23. Immunization, Isolation of Immunoglobulins, Estimation of Antibody Titre , 1973, Scandinavian journal of immunology. Supplement.

[5]  L. Navar,et al.  Pressure-mediated vasoconstriction of juxtamedullary afferent arterioles involves P2-purinoceptor activation. , 1996, The American journal of physiology.

[6]  A. Szent-Györgyi,et al.  The physiological activity of adenine compounds with especial reference to their action upon the mammalian heart 1 , 1929, The Journal of physiology.

[7]  G. Burnstock,et al.  Species differences in characteristics and distribution of [3H]α,β-methylene ATP binding sites in urinary bladder and urethra of rat, guinea-pig and rabbit , 1992 .

[8]  G. Burnstock.  Local mechanisms of blood flow control by perivascular nerves and endothelium. , 1990, Journal of hypertension. Supplement : official journal of the International Society of Hypertension.

[9]  P. Churchill,et al.  Pharmacological characterization of the renovascular P2 purinergic receptors. , 1993, The Journal of pharmacology and experimental therapeutics.

[10]  A. Vander,et al.  Effects of Adenosine Compounds on Renal Function and Renin Secretion in Dogs , 1970, Circulation research.

[11]  L. Navar,et al.  Calcium activation mechanisms in the renal microvascular response to extracellular ATP. , 1995, The American journal of physiology.

[12]  G. Burnstock,et al.  Purinoceptors: are there families of P2X and P2Y purinoceptors? , 1994, Pharmacology & therapeutics.

[13]  L. Bolis,et al.  Cell membrane receptors for drugs and hormones : a multidisciplinary approach , 1978 .

[14]  A. Haramati,et al.  Adenosine-induced decrease in renin release: dissociation from hemodynamic effects. , 1984, The American journal of physiology.

[15]  L. Navar,et al.  Extracellular ATP in the regulation of renal microvascular function1 , 1994, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[16]  G. Burnstock,et al.  Numbering of cloned P2 purinoceptors , 1996 .

[17]  L. Navar,et al.  Effects of ATP on pre- and postglomerular juxtamedullary microvasculature. , 1992, The American journal of physiology.

[18]  L. Navar,et al.  Juxtamedullary Afferent Arteriolar Responses to P1 and P2 Purinergic Stimulation , 1991, Hypertension.

[19]  L. Navar,et al.  Modulation of tubuloglomerular feedback responsiveness by extracellular ATP. , 1993, The American journal of physiology.

[20]  J. L. Gordon.  Extracellular ATP: effects, sources and fate. , 1986, The Biochemical journal.

[21]  J. Lorenz,et al.  Vasomotor effects of purinergic agonists in isolated rabbit afferent arterioles. , 1992, The American journal of physiology.

[22]  G. Burnstock,et al.  [3H]-α,β-Methylene ATP, a radioligand labelling P2-purinoceptors , 1989 .

[23]  J. Pearson,et al.  Cardiovascular purinoceptors. , 1990, Physiological reviews.

[24]  G. Burnstock.  A basis for distinguishing two types of purinergic receptor , 1978 .

[25]  G. Burnstock,et al.  Heterogeneous distribution of [3H]alpha,beta-methylene ATP binding sites in blood vessels. , 1993, Journal of vascular research.

[26]  G. Burnstock,et al.  A P2X purinoceptor expressed by a subset of sensory neurons , 1995, Nature.

[27]  M. Eltze,et al.  Characterization of vascular P2 purinoceptors in the rat isolated perfused kidney. , 1996, European journal of pharmacology.

[28]  S. Majid,et al.  Paracrine regulation of the renal microcirculation. , 1996, Physiological reviews.