Ozonation of Cowpea Grains: Alternative for the Control of Callosobruchus maculatus and Maintenance of Grain Quality

Nowadays, the modified atmosphere with ozone is one of the main alternatives for controlling insect pests in stored grains, as it allows grain quality to be maintained while causing no environmental damage. In light of this, the purpose of this study was to assess the toxicity of ozone to C. maculatus in cowpea grains as well as how it affects the physiological and physical properties of the grains. The toxicity was determined by estimating lethal doses (mg/g) at the top and bottom of the fumigation chamber for 50% and 95% of adult insects (LD50 and LD95). Cowpea grain samples were removed after being exposed to ozone, and the effect of ozonation on the physical and physiological quality of the grains was assessed. The electrical conductivity, water content, and germination were all examined in this manner. The lethal doses LD50 were 65.97 and 45.52 mg/g when the insects were distributed at the top and bottom of the fumigation chamber, respectively. As a result, ozone toxicity was higher at the bottom than at the top. The increase in ozone doses resulted in a decrease in water content. The germination rate exceeded 90% in both locations where ozone was applied. The electrical conductivity remained constant, ranging between 152.01 and 239.59 S cm−1 g −1. The findings indicate that ozone is toxic to C. maculatus but has no effect on grain quality. Ozone may therefore be used to manage C. maculatus in cowpea that has been stored.

[1]  G. N. Silva,et al.  Ozone as an alternative fumigant for controlling Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae) in cowpea beans , 2022, Journal of Stored Products Research.

[2]  D. Barbosa,et al.  Lethal and sublethal effects of chemical constituents from essential oils on Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae: Bruchinae) in cowpea stored grains , 2021, Journal of Plant Diseases and Protection.

[3]  Krishna Kumar Singh,et al.  Delineating the effect of gaseous ozone on disinfestation efficacy, protein quality, dehulling efficiency, cooking time and surface morphology of chickpea grains during storage , 2021 .

[4]  G. N. Silva,et al.  Ozonation of Lima Bean (Phaseolus Lunatus L.): Control of Zabrotes Subfasciatus (Boheman, 1833) (Coleoptera: Chrysomelidae: Bruchinae) and Maintenance of Grain Quality , 2021, Ozone: Science & Engineering.

[5]  C. S. Bastos,et al.  Physiological Quality of Corn Seeds Treated with Gaseous Ozone , 2021, Ozone: Science & Engineering.

[6]  D. Barbosa,et al.  Essential oils from Betula lenta, Cinnamomum cassia, Citrus aurantium var. Amara and Acorus calamus as biopesticides against cowpea weevil , 2021, International Journal of Tropical Insect Science.

[7]  R. Zinhoum,et al.  Efficacy of Ozone on Mortality, Super Oxide Dismutases, Nitric Oxide Enzymes and Ultrastructural Alterations on Some Stored Product Insect Larvae in Egypt , 2021, African Entomology.

[8]  S. Abdelgaleil,et al.  Toxicity of gaseous ozone to the different life stages of cowpea beetle, Callosobruchus maculatus (Coleoptera: Bruchidae), under laboratory conditions , 2021 .

[9]  Esmaeel Seyedabadi,et al.  Application of ozone against the larvae of Plodia interpunctella (Hübner) and its impacts on the organoleptic properties of walnuts. , 2020, Journal of food protection.

[10]  G. N. Silva,et al.  Chemical composition and insecticidal effect of essential oils from Illicium verum and Eugenia caryophyllus on Callosobruchus maculatus in cowpea , 2020 .

[11]  Márcio Antônio Mendonça,et al.  Ozonation of Brazil nuts: Decomposition kinetics, control of Aspergillus flavus and the effect on color and on raw oil quality , 2020 .

[12]  B. Subramanyam,et al.  Efficacy of ozone against adults and immature stages of phosphine susceptible and resistant strains of Rhyzopertha dominica , 2019, Journal of Stored Products Research.

[13]  L. Faroni,et al.  Kinetics of the ozone gas reaction in popcorn kernels , 2019, Journal of Stored Products Research.

[14]  D. Christ,et al.  Evaluation of grain and oil quality of packaged and ozonized flaxseed , 2019, Journal of Stored Products Research.

[15]  I. Khan,et al.  Host Selection Behavior of the Green Peach Aphid, Myzus persicae, in Response to Volatile Organic Compounds and Nitrogen Contents of Cabbage Cultivars , 2019, Front. Plant Sci..

[16]  S. Shahir,et al.  Ozone based food preservation: a promising green technology for enhanced food safety , 2018, Ozone: Science & Engineering.

[17]  G. N. Silva,et al.  Development rates of Callosobruchus maculatus (Coleoptera: Chrysomelidae) in landrace cowpea varieties occurring in southwestern Amazonia , 2018 .

[18]  Lêlisângela Carvalho da Silva,et al.  Rendimento de grãos secos e componentes de produção de genótipos de feijão-caupi em cultivo irrigado e de sequeiro , 2017 .

[19]  G. N. Silva,et al.  Ozone to control Rhyzopertha dominica (Coleoptera: Bostrichidae) in stored wheat grains , 2016 .

[20]  C. Athanassiou,et al.  The use of ozone gas for the control of insects and micro-organisms in stored products , 2015 .

[21]  J. Leeuwen Proposed OS&E Requirement: Measuring Ozone Dosage , 2015 .

[22]  L. Murdock,et al.  Effective and economic storage of pigeonpea seed in triple layer plastic bags , 2014 .

[23]  S Horvitz,et al.  Application of Ozone for the Postharvest Treatment of Fruits and Vegetables , 2014, Critical reviews in food science and nutrition.

[24]  K. Zhu‐Salzman,et al.  CO2 enhances effects of hypoxia on mortality, development, and gene expression in cowpea bruchid, Callosobruchus maculatus. , 2013, Journal of insect physiology.

[25]  A. H. Sousa,et al.  Ozone Toxicity and Walking Response of Populations of Sitophilus zeamais (Coleoptera: Curculionidae) , 2012, Journal of economic entomology.

[26]  R. T. Noyes,et al.  Ozone fumigation of stored grain; closed-loop recirculation and the rate of ozone consumption , 2010 .

[27]  S. Öztekin,et al.  Comparison of susceptibility of two stored-product insects, Ephestia kuehniella Zeller and Tribolium confusum du Val to gaseous ozone , 2009 .

[28]  D. Maier,et al.  Efficacy and fumigation characteristics of ozone in stored maize. , 2001, Journal of stored products research.

[29]  A. Yousef,et al.  Application of ozone for enhancing the microbiological safety and quality of foods: a review. , 1999, Journal of food protection.

[30]  J. Kleperis,et al.  Evaluation of ozone influence on wheat grain quality during active drying , 2021 .

[31]  Raul Narciso C. Guedes,et al.  Phosphine resistance in Brazilian populations of Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) , 2009 .

[32]  D. Maier,et al.  Penetration of ozone into columns of stored grains and effects on chemical composition and processing performance , 2003 .

[33]  B. Langlais,et al.  Guideline for Measurement of Ozone Concentration in the Process Gas From an Ozone Generator , 1996 .