Cybernetic modeling of bacterial cultures at low growth rates: Mixed‐substrate systems

A cybernetic model to predict the low‐growth‐rate behavior of bacteria in mixed‐substrate environment is presented. Using only growth and maintenance parameters from single‐substrate experiments, the model accurately predicts the simultaneous substrate utilization and maintenance energy effects in constant fed‐batch cultures of Klebsiella oxytoca. The robustness of the model was examined more rigorously by perturbing glucose‐limited fed‐batch cultures with additions of arabinose, xylose, and fructose. In all cases, reasonable agreement of the model prediction with the experimental data was observed.

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