Photon Burst Detection of Single Near-Infrared Fluorescent Molecules

The ability to observe the burst of photons from single fluorescent molecules in solution with high efficiency (>90%) is a technically challenging task and has important applications in many areas of analytical chemistry. The ability to observe the photon burst from visible fluorescent dye molecules using pulsed-laser excitation and time-gated detection in order to reduce the scattered photon contribution from the background has recently been demonstrated. The detection efficiency of these molecules was limited due to the excitation of fluorescent impurities resulting in small amplitude bursts observed in the solvent blank requiring the need for a high discriminator threshold to reduce the number of errors from false positives

[1]  Ambrose,et al.  Comment on "Single pentacene molecules detected by fluorescence excitation in a p-terphenyl crystal" , 1991, Physical review letters.

[2]  Gabor Patonay,et al.  Near-Infrared Fluorogenic Labels: New Approach to an Old Problem , 1991 .

[3]  James H. Jett,et al.  SINGLE-MOLECULE DETECTION OF RHODAMINE 6G IN ETHANOLIC SOLUTIONS USING CONTINUOUS WAVE LASER EXCITATION , 1991 .

[4]  W. P. Ambrose,et al.  Fluorescence spectroscopy and spectral diffusion of single impurity molecules in a crystal , 1991, Nature.

[5]  Steven A. Soper,et al.  Detection of single fluorescent molecules , 1990 .

[6]  M. Orrit,et al.  Single pentacene molecules detected by fluorescence excitation in a p-terphenyl crystal. , 1990, Physical review letters.

[7]  P. A. Johnson,et al.  Ultralow Detection Limit of a Near-Infrared Dye by Diode-Laser-Induced Fluorescence in a Flowing Stream , 1990 .

[8]  R A Mathies,et al.  Optimization of high-sensitivity fluorescence detection. , 1990, Analytical chemistry.

[9]  T. Imasaka,et al.  Diode Lasers AND PRACTICAL TRACE ANALYSIS , 1990 .

[10]  R A Mathies,et al.  Single-molecule fluorescence detection: autocorrelation criterion and experimental realization with phycoerythrin. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[11]  Paul A. Johnson,et al.  Ultra-low detection limits for an organic dye determined by fluorescence spectroscopy with laser diode excitation , 1989 .

[12]  N. Dovichi,et al.  Subattomole amino acid analysis by capillary zone electrophoresis and laser-induced fluorescence. , 1988, Science.

[13]  Sergio Cova,et al.  Performance comparison of a single‐photon avalanche diode with a microchannel‐plate photomultiplier in time‐correlated single‐photon counting , 1988 .

[14]  L. Stryer,et al.  Photostability studies of phycobiliprotein fluorescent labels. , 1987, Analytical biochemistry.

[15]  M. Walport,et al.  Molecular calibration in flow cytometry with sub-attogram detection limit. , 1986, Journal of immunological methods.

[16]  N. Ishibashi,et al.  Determination of protein in human serum by high-performance liquid chromatography with semiconductor laser fluorometric detection. , 1986, Analytical chemistry.

[17]  Totaro Imasaka,et al.  Pulsed semiconductor laser fluorometry for lifetime measurements , 1985 .

[18]  J H Jett,et al.  Laser-induced fluorescence of flowing samples as an approach to single-molecule detection in liquids. , 1984, Analytical chemistry.

[19]  S. Cova,et al.  Towards picosecond resolution with single-photon avalanche diodes , 1981 .

[20]  C. T. J. Alkemade.  Single-Atom Detection , 1981 .

[21]  G. S. Hurst,et al.  One-atom detection using resonance ionization spectroscopy , 1977 .

[22]  T. Hirschfeld.  Optical microscopic observation of single small molecules. , 1976, Applied optics.