Molecular Detection and Analysis of Blast Resistance Genes in Rice Main Varieties in Jiangsu Province, China

Rice blast, caused by Pyricularia oryzae, is one of the most destructive rice diseases worldwide. Using resistant rice varieties is the most cost-effective way to control the disease, and it is crucial to analyze the resistance level and the resistance genes distribution of the main varieties. In this study, we collected 119 rice main varieties in Jiangsu province and evaluated the resistance to leaf and panicle blast and found that indica rice was more resistant to rice blast than japonica rice. Moreover, we detected the distribution of 14 resistance genes (R genes) in the 119 varieties. The distribution frequencies of three R genes, Pish, Pit, and Pia, were higher than 80%, and the Pigm had the lowest distribution frequency (1.68%), followed by Pi2 (15.18%) and Pi5, Piz-t (24.37%). Combined with the multiple stepwise regression and the resistance contribution rate, eight major R genes Pita, Pi5, Pi9, Pib, Pb1, Pikm, Piz-t, and Pi2 significantly affected the resistance of rice, and we also found that six gene combinations with 100% resistance contribution rate could effectively increase the resistance of rice varieties. In summary, monitoring the resistance level of rice varieties and analyzing their resistance genes were beneficial for rice resistance breeding.

[1]  Ping Wang,et al.  Co-evolved ascorbate oxidases of plant and the blast fungus orchestrate host apoplast redox state to modulate rice immunity. , 2022, Molecular plant.

[2]  Z. Qi,et al.  Effects of panicle development stage and temperature on rice panicle blast infection by Magnaporthe oryzae and visualization of its infection process , 2021 .

[3]  Y. Dong,et al.  Evaluation of suitable reference genes for normalization of quantitative real-time PCR analysis in rice plants under Xanthomonas oryzae pv. oryzae--infection and melatonin supplementation , 2020 .

[4]  Z. Qi,et al.  Pathogenicity and population structure analysis of Pyricularia oryzae in different districts of Jiangsu province, China , 2020 .

[5]  Xuewei Chen,et al.  Recent advances in broad-spectrum resistance to the rice blast disease. , 2019, Current opinion in plant biology.

[6]  Huang Qingfeng,et al.  Effects of multiple genes polymerization on rice blast resistance in Jiangsu Province and the development of functional markers of Pb1 gene. , 2019 .

[7]  R. Terauchi,et al.  Polymorphic residues in rice NLRs expand binding and response to effectors of the blast pathogen , 2018, Nature Plants.

[8]  Huanhuan Li,et al.  Enhanced resistance to rice blast and sheath blight in rice (oryza sativa L.) by expressing the oxalate decarboxylase protein Bacisubin from Bacillus subtilis. , 2017, Plant science : an international journal of experimental plant biology.

[9]  S. Munda,et al.  Correction: Use of molecular markers in identification and characterization of resistance to rice blast in India , 2017, PLoS ONE.

[10]  Ertao Wang,et al.  Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance , 2017, Science.

[11]  Xu Jian-long,et al.  Identification and Evaluation of Blast Resistance for Resequenced Rice Core Collections , 2017 .

[12]  Yulin Jia,et al.  Identification of a Pi9-Containing Rice Germplasm with a Newly Developed Robust Marker. , 2016, Phytopathology.

[13]  Dai Liang-ying,et al.  I mproving Blast Resistance of Rice Restorer R288 by Molecular Marker -Assisted Selection of Pi9 Gene , 2016 .

[14]  Xuebiao Pan,et al.  Combination Patterns of Major R Genes Determine the Level of Resistance to the M. oryzae in Rice (Oryza sativa L.) , 2015, PloS one.

[15]  T. Sharma,et al.  Genetic and physical mapping of a new allele of Pik locus from japonica rice ‘Liziangxintuanheigu’ , 2015, Euphytica.

[16]  N. Yao,et al.  Function and Interaction of the Coupled Genes Responsible for Pik-h Encoded Rice Blast Resistance , 2014, PloS one.

[17]  S. Saud,et al.  Disease resistance in rice and the role of molecular breeding in protecting rice crops against diseases , 2014, Biotechnology Letters.

[18]  Sihai Yang,et al.  Rapid evolution of avirulence genes in rice blast fungus Magnaporthe oryzae , 2014, BMC Genetics.

[19]  R. Terauchi,et al.  The Rice Resistance Protein Pair RGA4/RGA5 Recognizes the Magnaporthe oryzae Effectors AVR-Pia and AVR1-CO39 by Direct Binding[W][OA] , 2013, Plant Cell.

[20]  V. Shenoy,et al.  Marker-assisted breeding of Pi-1 and Piz-5 genes imparting resistance to rice blast in PRR78, restorer line of Pusa RH-10 Basmati rice hybrid , 2013 .

[21]  Cai-guo Xu,et al.  Improving blast resistance of Jin 23B and its hybrid rice by marker-assisted gene pyramiding , 2012, Molecular Breeding.

[22]  I. Ashikawa,et al.  The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast , 2012, Theoretical and Applied Genetics.

[23]  M. Jia,et al.  Analysis of rice blast resistance gene Pi-z in rice germplasm using pathogenicity assays and DNA markers , 2012, Euphytica.

[24]  H. Matsumura,et al.  A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes. , 2011, The Plant journal : for cell and molecular biology.

[25]  Y. Da-zhao.  Diversity analysis for resistance of 251 rice(Oryza sativa L.) varieties(lines) to rice blast disease , 2011 .

[26]  T. Sharma,et al.  Functional complementation of rice blast resistance gene Pi-kh(Pi54) conferring resistance to diverse strains of Magnaporthe oryzae , 2011, Journal of Plant Biochemistry and Biotechnology.

[27]  D. Fujita,et al.  Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.) , 2010 .

[28]  M. Yano,et al.  Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication. , 2010, The Plant journal : for cell and molecular biology.

[29]  Y. Fujita,et al.  Identification of the blast resistance gene Pit in rice cultivars using functional markers , 2010, Theoretical and Applied Genetics.

[30]  Guo-Liang Wang,et al.  Recent progress and understanding of the molecular mechanisms of the rice-Magnaporthe oryzae interaction. , 2010, Molecular plant pathology.

[31]  S. Urso,et al.  Polymorphism analysis of genomic regions associated with broad-spectrum effective blast resistance genes for marker development in rice , 2010, Molecular Breeding.

[32]  B. C. Viraktamath,et al.  Development and validation of functional marker targeting an InDel in the major rice blast disease resistance gene Pi54 (Pikh) , 2010, Molecular Breeding.

[33]  Norikuni Saka,et al.  Loss of Function of a Proline-Containing Protein Confers Durable Disease Resistance in Rice , 2009, Science.

[34]  N. Talbot,et al.  Under pressure: investigating the biology of plant infection by Magnaporthe oryzae , 2009, Nature Reviews Microbiology.

[35]  Seogchan Kang,et al.  Genome organization and evolution of the AVR-Pita avirulence gene family in the Magnaporthe grisea species complex. , 2008, Molecular plant-microbe interactions : MPMI.

[36]  A. McClung,et al.  Development of DNA Markers Suitable for Marker Assisted Selection of Three Pi Genes Conferring Resistance to Multiple Pyricularia grisea Pathotypes , 2004 .

[37]  Zhonghua Wang,et al.  Development of a SNLP marker from the Pi-ta blast resistance gene by tri-primer PCR , 2004, Euphytica.

[38]  H. Leung,et al.  Mapping of leaf and neck blast resistance genes with resistance gene analog, RAPD and RFLP in rice , 2002, Euphytica.

[39]  Thomas Lübberstedt,et al.  Functional markers in plants. , 2003, Trends in plant science.

[40]  G. Wang,et al.  Genetic and physical mapping of Pi5(t), a locus associated with broad-spectrum resistance to rice blast , 2003, Molecular Genetics and Genomics.

[41]  H. Yuqing,et al.  Gene pyramiding to increase the blast resistance in rice , 2003 .

[42]  L. Kohn,et al.  A multilocus gene genealogy concordant with host preference indicates segregation of a new species, Magnaporthe oryzae, from M. grisea , 2002 .

[43]  Apichart Vanavichit,et al.  Quantitative trait loci associated with leaf and neck blast resistance in recombinant inbred line population of rice (Oryza sativa). , 2002, DNA research : an international journal for rapid publication of reports on genes and genomes.

[44]  R. Tarchini,et al.  A Single Amino Acid Difference Distinguishes Resistant and Susceptible Alleles of the Rice Blast Resistance Gene Pi-ta , 2000, Plant Cell.

[45]  A. Parco,et al.  Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice , 2000, Theoretical and Applied Genetics.

[46]  Jonathan D. G. Jones,et al.  The NB-ARC domain: a novel signalling motif shared by plant resistance gene products and regulators of cell death in animals , 1998, Current Biology.

[47]  J. Nottéghem,et al.  Evidence of a gene-for-gene relationship in the Oryza sativa-Magnaporthe grisea pathosystem , 1992 .

[48]  H H Flor,et al.  Current Status of the Gene-For-Gene Concept , 1971 .