Development of sulphide-selective optode membranes based on fluorescence quenching

Abstract Sulphide-selective optode membranes have been fabricated by immobilizing tetraoctylammonium fluorescein mercury(II) acetate (TOAFMA), tetraoctylammonium hydroxide (TOAOH) and tributyl phosphate into ethyl cellulose and these membranes have been used in sensing devices for optical determination of sulphide ions in pH buffered solutions. The sensing scheme is based on the fluorescence quenching of TOAFMA by hydrosulphide ions, which can be related to the concentration of the sulphide ions present in solution. The response behaviour of the membrane is pH dependent and has a wider linear response range at high pH values. At pH 9.00, 10.0 and 11.0 the useful ranges are 1.0 to 20 μM, 0.10 to 10 μM and 0.10 to 100 μM, respectively. The detection limit is 56 nM sulphide ion at pH 11.0. Interferences, response time and reproducibility are discussed.

[1]  F. Karush,et al.  AN ASSAY METHOD FOR DISULFIDE GROUPS BY FLUORESCENCE QUENCHING. , 1964, Analytical biochemistry.

[2]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[3]  James E. Huheey,et al.  Inorganic chemistry; principles of structure and reactivity , 1972 .

[4]  B. Weigl,et al.  New hydrophobic materials for optical carbon dioxide sensors based on ion pairing , 1995 .

[5]  E. Pretsch,et al.  Detection limit of ion-selective bulk optodes and corresponding electrodes , 1993 .

[6]  W. M. Haynes.  CRC Handbook of Chemistry and Physics , 1990 .

[7]  J. Lodge,et al.  Fluorescence determinatioof sub-parts per billion hydrogen sulfide in the atmosphere. , 1969, Analytical Chemistry.

[8]  M. Biggiogera,et al.  Fluorescein mercuric acetate as a dye for fluorescence and electron microscope detection of -SH groups in mouse sperm protamines: a critical evaluation. , 1988, Acta histochemica.

[9]  R. Raiswell,et al.  A simple method for field determination of some reduced sulphur species in soil gases , 1991 .

[10]  T. R. Andrew,et al.  The determination of hydrogen sulphide in the atmosphere , 1965 .

[11]  M. Shahriari,et al.  Active silica-gel films for hydrogen sulfide optical sensor application. , 1994, Optics letters.

[12]  M. Jacobs,et al.  Ultramicrodetermination of Sulfides in Air , 1957 .

[13]  G. J. Maclay,et al.  An optical waveguide acid vapor sensor. , 1992, Talanta.