Capillary electrochromatography of peptides on microfabricated poly(dimethylsiloxane) chips modified by cerium(IV)-catalyzed polymerization.

Vinylsulfonic acid, acrylic acid, 2-acrylamido-2-methylpropanesulfonic acid (AMPS), 4-styrenesulfonic acid, and stearyl methacrylate were used for successful modification of the surface of poly(dimethylsiloxane) (PDMS) by cerium(IV) catalyzed polymerization on microfabricated collocated monolith support structures microchips. Reproducible and stable coatings were obtained allowing highly efficient separations of a peptide mixture with RSD for retention times below 2.6%. AMPS-coated PDMS channels were shown to give a reproducible separation of a synthetic peptide mixture for over a month. Subsequent modification of microchip channels by AMPS and methoxydimethyloctadecylsilane allowed selective separation of complex bovine serum albumin digest with high reproducibility, and efficiency of about 620,000 plates/m.

[1]  M. Hearn,et al.  Capillary electroendoosmotic chromatography of peptides. , 2000, Journal of chromatography. A.

[2]  F. Regnier,et al.  Microfabricated liquid chromatography columns based on collocated monolith support structures. , 1998, Journal of pharmaceutical and biomedical analysis.

[3]  F. Regnier,et al.  A picoliter-volume mixer for microfluidic analytical systems. , 2001, Analytical chemistry.

[4]  C. Bamford,et al.  Polymer surface functionalisation and grafting by a simple and inexpensive method , 1994 .

[5]  L. Sander,et al.  Stationary phases for capillary electrochromatography. , 2000, Journal of chromatography. A.

[6]  K. Bartle,et al.  Capillary electrochromatography: A review , 1997 .

[7]  K. Altria.  Overview of capillary electrophoresis and capillary electrochromatography. , 1999, Journal of chromatography. A.

[8]  W. Hancock.  CRC handbook of HPLC for the separation of amino acids, peptides, and proteins , 1984 .

[9]  John H. Knox,et al.  Electrochromatography in packed tubes using 1.5 to 50 μm silica gels and ODS bonded silica gels , 1991 .

[10]  T. Shepodd,et al.  Conduct-as-cast polymer monoliths as separation media for capillary electrochromatography. , 2001, Analytical chemistry.

[11]  I. Gusev,et al.  Capillary columns with in situ formed porous monolithic packing for micro high-performance liquid chromatography and capillary electrochromatography. , 1999, Journal of chromatography. A.

[12]  F. Švec,et al.  Fast Ion‐Exchange HPLC of Proteins Using Porous Poly(glycidyl methacrylate‐co‐ethylene dimethacrylate) Monoliths Grafted with Poly(2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid) , 1997, Biotechnology progress.

[13]  S. Hjertén,et al.  Electroosmosis- and pressure-driven chromatography in chips using continuous beds. , 2000, Analytical chemistry.

[14]  M Petro,et al.  Molded rigid polymer monoliths as separation media for capillary electrochromatography. 1. Fine control of porous properties and surface chemistry. , 1998, Analytical chemistry.

[15]  C. Fujimoto.  Packing materials and separation efficiencies in capillary electrochromatography , 1999 .

[16]  R. Oleschuk,et al.  Trapping of bead-based reagents within microfluidic systems: on-chip solid-phase extraction and electrochromatography , 2000, Analytical chemistry.

[17]  F. Regnier.  Microfabricated Monolith Columns for Liquid Chromatography. Sculpting Supports for Liquid Chromatography , 2000 .

[18]  N Tait,et al.  Fabrication of nanocolumns for liquid chromatography. , 1998, Analytical chemistry.

[19]  F. Švec,et al.  Molded rigid polymer monoliths as separation media for capillary electrochromatography. 2. Effect of chromatographic conditions on the separation. , 1998, Analytical chemistry.

[20]  S. Kaizerman,et al.  A new method for the preparation of graft copolymers. Polymerization initiated by ceric ion redox systems , 1958 .

[21]  K. Jinno.  Recent trends in open-tubular capillary electrochromatography , 2000 .

[22]  S. R. Shukla,et al.  Graft‐copolymerization of glycidyl methacrylate onto cotton cellulose , 1994 .

[23]  S. Hjertén.  Standard and Capillary Chromatography, Including Electrochromatography, on Continuous Polymer Beds (Monoliths), Based on Water-Soluble Monomers , 1999 .

[24]  F. Regnier,et al.  Capillary electrochromatography of peptides in a microfabricated system. , 1999, Journal of chromatography. A.

[25]  Jörg Peter Kutter,et al.  Current developments in electrophoretic and chromatographic separation methods on microfabricated devices , 2000 .

[26]  J. Thompson,et al.  Fast analytical-scale separations by capillary electrophoresis and liquid chromatography. , 1999, Chemical reviews.

[27]  N. Penner,et al.  Nanoliter capillary electrochromatography columns based on collocated monolithic support structures molded in poly(dimethyl siloxane) , 2001, Electrophoresis.

[28]  G. Whitesides,et al.  Fabrication of microfluidic systems in poly(dimethylsiloxane) , 2000, Electrophoresis.

[29]  F. Švec,et al.  Towards stationary phases for chromatography on a microchip: Molded porous polymer monoliths prepared in capillaries by photoinitiated in situ polymerization as separation media for electrochromatography , 2000, Electrophoresis.

[30]  Frantisek Svec,et al.  Monolithic Stationary Phases for Capillary Electrochromatography Based on Synthetic Polymers: Designs and Applications , 2000 .

[31]  J. Michael Ramsey,et al.  Microchip Capillary Electrophoresis with an Integrated Postcolumn Reactor , 1994 .