I. Supporting Information

Fig. S3 – Specificity of the Grx3 siRNA-induced phenotype in HeLa cells To confirm the specificity of the siRNA used in this study (si-Grx3-1, blue in C), we compared different aspects of the depletion phenotype with that of HeLa cells transfected with the identical siRNA (si-Grx3-1*) from an alternative supplier (Ambion vs Eurogentec) as well as with a pool of four alternative siRNAs (si-Grx3-pool, red in C, supplier Dharmacon, accession numbers: J019954-09,-10, -11, and -12). (A) Immunostaining of the indicated proteins extracted from HeLa cells transfected with scrambled control RNA (si-scr) or different siRNAs. (B) The activity of cytosolic aconitase normalized to lactate dehydrogenase activity was measured in samples of part A relative to the control cells.

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