Myofibroblasts in healing laser wounds of rat tongue mucosa.

The distribution of myofibroblasts was studied in healing laser incisions compared with scalpel-incision and excision wounds in dorsal tongue mucosa and excision wounds in back skin. Myofibroblasts (m-f-b) were visualized by staining with NBD-phallacidin, a fluorescent probe specific for F-actin, and by electron microscopy. Few, randomly-orientated m-f-b were found in laser wounds over 28 days. Neither m-f-b nor contraction were seen in the scalpel-incisions. No contraction was observed in the laser wounds whereas an organized network of m-f-b with substantial contraction occurred in excision wounds. It is suggested that lack of contraction in laser wounds is partially due to the fewness of m-f-b. The residual connective tissue matrix resisting the laser treatment also seems to play a role in preventing the wound contraction.

[1]  B. Hirschel,et al.  GRANULATION TISSUE AS A CONTRACTILE ORGAN: A STUDY OF STRUCTURE AND FUNCTION , 1972 .

[2]  J. Frame.  Treatment of sublingual keratosis with the CO2 laser , 1984, British Dental Journal.

[3]  J. E. Heaysman,et al.  Early contacts between fibroblasts. An ultrastructural study. , 1973, Experimental cell research.

[4]  E. Baker,et al.  Incision of Tissue by Carbon Dioxide Laser , 1971, Nature.

[5]  I. Virtanen,et al.  Healing of laser and scalpel incision wounds of rat tongue mucosa as studied with cytokeratin antibodies. , 1987, Journal of oral pathology.

[6]  R M Browne,et al.  A comparative histological study of wound healing following CO2 laser and conventional surgical excision of canine buccal mucosa. , 1983, Archives of oral biology.

[7]  S. Cohen,et al.  Epidermal growth factor: effects of androgens and adrenergic agents. , 1974, Endocrinology.

[8]  D. L. Taylor,et al.  Fluorescently labelled molecules as probes of the structure and function of living cells , 1980, Nature.

[9]  G. Majno.  The story of the myofibroblasts , 1979, The American journal of surgical pathology.

[10]  H. Faulstich,et al.  Amatoxins, phallotoxins, phallolysin, and antamanide: the biologically active components of poisonous Amanita mushrooms. , 1978, CRC critical reviews in biochemistry.

[11]  W. Webb,et al.  Fluorescence staining of the actin cytoskeleton in living cells with 7-nitrobenz-2-oxa-1,3-diazole-phallacidin. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[12]  S. Cohen,et al.  Epidermal growth factor , 1972, The Journal of investigative dermatology.

[13]  R. Verschueren,et al.  CO2 Laser Surgery , 1980 .

[14]  M. White,et al.  Epithelial wound healing enhanced by transforming growth factor-alpha and vaccinia growth factor. , 1987, Science.

[15]  J. Meurman,et al.  Laser-induced alterations in rat oral mucosa. , 1986, Scandinavian journal of dental research.

[16]  L. King.  What does epidermal growth factor do and how does it do it? , 1985, The Journal of investigative dermatology.

[17]  R. Wallace,et al.  Laser surgery in the aerodigestive tract. , 1973, American journal of surgery.

[18]  H. Ehrlich,et al.  A comparative study of fibroblasts in healing freeze and burn injuries in rats. , 1984, The American journal of pathology.

[19]  Arthur K. C. Li,et al.  Differences in Healing of Skin Wounds Caused by Burn and Freeze Injuries , 1980, Annals of surgery.

[20]  A. Swann.  (Na+,K+)-ATPase stimulation by sucrose feeding: prevention by 6-hydroxydopamine. , 1984, Life sciences.

[21]  G. Gabbiani,et al.  Reparative processes in mammalian wound healing: the role of contractile phenomena. , 1977, International review of cytology.

[22]  R. Ross.  THE FIBROBLAST AND WOUND REPAIR , 1968, Biological reviews of the Cambridge Philosophical Society.

[23]  J W Frame,et al.  Carbon dioxide laser surgery for benign oral lesions , 1985, British Dental Journal.

[24]  J. Meurman,et al.  Extracellular matrix in healing CO2 laser incision wound. , 1987, Journal of oral pathology.

[25]  J. Carruth,et al.  The carbon dioxide surgical laser , 1980, British Dental Journal.

[26]  G. Gabbiani,et al.  The role of cytoskeletal and cytocontractile elements in pathologic processes. , 1983, The American journal of pathology.

[27]  J. Atkins,et al.  CO2 laser surgery of the upper airway. , 1984, The Surgical clinics of North America.

[28]  G. Gabbiani,et al.  Cytoplasmic filaments and gap junctions in epithelial cells and myofibroblasts during wound healing , 1978, The Journal of cell biology.

[29]  S. Stein.  CO2 laser surgery of the cervix, vagina, and vulva. , 1984, The Surgical clinics of North America.

[30]  S. Hundahl,et al.  The Spatial and Temporal Quantification of Myofibroblasts , 1982, Plastic and reconstructive surgery.

[31]  M Luomanen,et al.  A comparative study of healing of laser and scalpel incision wounds in rat oral mucosa. , 1987, Scandinavian journal of dental research.