Epidermal growth factor receptor expression in oligodendroglial tumors.

A series of 13 oligodendrogliomas (WHO grade II) and 20 anaplastic oligodendrogliomas (WHO grade III) was studied for gene amplification and expression of the epidermal growth factor receptor gene (EGFR). EGFR gene amplification was found in only one case of anaplastic oligodendroglioma, which additionally showed a deletion/rearrangement at the 5' end of the gene. Northern blot analysis, however, revealed increases of EGFR mRNA expression relative to non-neoplastic control brain in 6 of 13 oligodendrogliomas and 10 of 18 anaplastic oligodendrogliomas. All cases with increased mRNA expression showed strong immunoreactivity for EGFR protein. Our findings thus indicate that increased expression of EGFR mRNA and protein is common in low-grade and high-grade oligodendroglial tumors and in the vast majority of cases is not caused by gene amplification.

[1]  J. Rey,et al.  Allelic loss at 1p and 19q frequently occurs in association and may represent early oncogenic events in oligodendroglial tumors , 1995, International journal of cancer.

[2]  D. Louis,et al.  Shared Allelic Losses on Chromosomes 1p and 19q Suggest a Common Origin of Oligodendroglioma and Oligoastrocytoma , 1995, Journal of neuropathology and experimental neurology.

[3]  M. Rosner,et al.  Induction of epidermal growth factor receptor gene transcription by transforming growth factor beta 1: association with loss of protein binding to a negative regulatory element. , 1994, Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research.

[4]  S. Deb,et al.  Wild-type human p53 activates the human epidermal growth factor receptor promoter. , 1994, Oncogene.

[5]  P. Zoltick,et al.  The molecular biology and molecular genetics of astrocytic neoplasms. , 1994, Seminars in oncology.

[6]  V. P. Collins,et al.  Epidermal growth factor receptor gene and its transcripts in glioblastomas. , 1994, Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer.

[7]  G. Reifenberger,et al.  Amplification and overexpression of the MDM2 gene in a subset of human malignant gliomas without p53 mutations. , 1993, Cancer research.

[8]  V. Collins.  Amplified genes in human gliomas. , 1993, Seminars in cancer biology.

[9]  K. Haruma,et al.  The level of a transcription factor Sp1 is correlated with the expression of EGF receptor in human gastric carcinomas. , 1992, Biochemical and biophysical research communications.

[10]  E. Oldfield,et al.  Amplification and/or overexpression of platelet-derived growth factor receptors and epidermal growth factor receptor in human glial tumors. , 1992, Cancer research.

[11]  C. James,et al.  Amplified and rearranged epidermal growth factor receptor genes in human glioblastomas reveal deletions of sequences encoding portions of the N- and/or C-terminal tails. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[12]  G. Fuller,et al.  Amplified cellular oncogenes in neoplasms of the human central nervous system. , 1992, Mutation research.

[13]  P. Humphrey,et al.  Structural alterations of the epidermal growth factor receptor gene in human gliomas. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[14]  A. Benabid,et al.  EGF receptor amplification and expression in human brain tumours. , 1992, European journal of cancer.

[15]  A. Hamburger,et al.  Increased epidermal growth factor receptor gene expression by gamma-interferon in a human breast carcinoma cell line. , 1991, British Journal of Cancer.

[16]  C. James,et al.  Genes for epidermal growth factor receptor, transforming growth factor alpha, and epidermal growth factor and their expression in human gliomas in vivo. , 1991, Cancer research.

[17]  N. Lemoine,et al.  Avoiding Punishment Is Its Own Reward , 1991, British Journal of Cancer.

[18]  C. James,et al.  Identical splicing of aberrant epidermal growth factor receptor transcripts from amplified rearranged genes in human glioblastomas. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[19]  M. Shibuya,et al.  A Deletion Mutation within the Ligand Binding Domain Is Responsible for Activation of Epidermal Growth Factor Receptor Gene in Human Brain Tumors , 1990, Japanese journal of cancer research : Gann.

[20]  I. Pastan,et al.  A transcription factor active on the epidermal growth factor receptor gene. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[21]  A. Kaye,et al.  Selective amplification of the cytoplasmic domain of the epidermal growth factor receptor gene in glioblastoma multiforme. , 1988, Cancer research.

[22]  B. Vogelstein,et al.  Gene Amplification in Malignant Human Gliomas: Clinical and Histopathologic Aspects , 1988, Journal of neuropathology and experimental neurology.

[23]  A. Ullrich,et al.  Overexpression of the human EGF receptor confers an EGF-dependent transformed phenotype to NIH 3T3 cells , 1987, Cell.

[24]  K. Kinzler,et al.  Increased expression of the epidermal growth factor receptor gene in malignant gliomas is invariably associated with gene amplification. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[25]  Hermona Soreq,et al.  Amplification, enhanced expression and possible rearrangement of EGF receptor gene in primary human brain tumours of glial origin , 1985, Nature.

[26]  P. Seeburg,et al.  Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells , 1984, Nature.

[27]  Richard B. Richter,et al.  Pathology of Tumors of the Nervous System. , 1964 .