SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF TETRAZOLE DERIVATIVES

Article history Tetrazoles are an important class of heterocyclic compounds which possess wide spectrum of biological properties. A series of tetrazoles were synthesized by diazotization of aminoguanidine bicarbonate using nitric acid and sodium nitrate to yield guanylazide. The obtained diazotisation mixture was refluxed using sodium carbonate to obtain 5- aminotetrazole which was acetylated using acetyl chloride and condensed using substituded aromatic aldehydes. All the synthesized compounds were characterized by IR, 1H NMR and 13C NMR Spectroscopy. The compounds were evaluated for their antibacterial activity against Staphylococcus aureus Bacillus pimilis and Escherichia coli using streptomycin as reference standard and anti-fungal activity against Aspergillus niger and Pencillium notatum using miconazole as reference standard. Compounds TZ2 and TZ8 were found to exhibit highest activity against S.aureus and B.pimilis. The compounds TZ2 and TZ7 showed maximum activity against E. coli. The compounds TZ1 and TZ7 exhibited most potent activity against A. niger and P. notatum respectively.