论文信息 - A newly designed optical biochip for a TDM-POCT device

A newly designed optical biochip for a TDM-POCT device

The design of a novel therapeutic drug monitoring (TDM) point-of-care-testing (POCT) biochip for immunosuppressants detection in transplanted patients is described. The chip consists of two polymeric parts, a top PMMA slide and a bottom ZEONOR® thin foil, bonded together by means of a pressure sensitive adhesive tape. The tape, with lower refractive index, is shaped in order to obtain a microfluidic multi-channel array. The optical signal, coming from an external light source and travelling along the ZEONOR® thin foil, excites the fluorescent sensing layer immobilized onto the fluidic channels. Preliminary tests with the bioassay implementation for tacrolimus detection are reported.

[1] F Baldini,et al. A new optical platform for biosensing based on fluorescence anisotropy , 2008, Analytical and bioanalytical chemistry.

[2] Albrecht Brandenburg,et al. Biochip readout system for point-of-care applications , 2009 .

[3] Albrecht Brandenburg,et al. Modeling and experimental verification of the performance of TIRF-sensing systems for oligonucleotide microarrays based on bulk and integrated optical planar waveguides , 2003 .

[4] B. MacCraith,et al. Theory of the radiation of dipoles placed within a multilayer system. , 2000, Applied optics.

[5] G. Russ,et al. Comparison of Trough, 2-Hour, and Limited AUC Blood Sampling for Monitoring Cyclosporin (Neoral®) at Day 7 Post–Renal Transplantation and Incidence of Rejection in the First Month , 2002, Therapeutic drug monitoring.