3D perfusion mapping in post-infarct mice using myocardial contrast echocardiography.

Myocardial contrast echocardiography (MCE) was used to construct three-dimensional maps of perfusion defects in closed-chest mice, with and without myocardial infarction (MI) induced by permanent coronary ligation. Contiguous, short-axis MCE cine images spanning the heart from apex to base were acquired at 1 mm elevations in each mouse. MCE images at each elevation were color-coded to indicate relative perfusion and were compared with postmortem histology. A strong correlation (R > 0.93) in the size of perfused areas was observed between in vivo measurements and the results of conventional ex vivo tissue staining. 3D multislice and 3D surface renderings of perfusion distribution were created and these perfusion maps also matched well with postmortem histology. These methods provide for the noninvasive determination of the total ischemic region placed at risk by coronary occlusion: this is a critical variable in assessing the potential of novel therapeutic agents to reduce MI size in murine models of ischemia/reperfusion injury.

[1]  P. Hunziker,et al.  Echocardiographic determination of risk area size in a murine model of myocardial ischemia. , 1999, American journal of physiology. Heart and circulatory physiology.

[2]  P. Phillips,et al.  Contrast pulse sequences (CPS): imaging nonlinear microbubbles , 2001, 2001 IEEE Ultrasonics Symposium. Proceedings. An International Symposium (Cat. No.01CH37263).

[3]  John A. Hossack,et al.  Myocardial contrast echocardiography to assess perfusion in a mouse model of ischemia/reperfusion injury , 2004, SPIE Medical Imaging.

[4]  T. Porter,et al.  Transient myocardial contrast after initial exposure to diagnostic ultrasound pressures with minute doses of intravenously injected microbubbles. Demonstration and potential mechanisms. , 1995, Circulation.

[5]  W. Gilson,et al.  Serial MRI evaluation of cardiac structure and function in mice after reperfused myocardial infarction , 2002, Magnetic resonance in medicine.

[6]  R. Vergona,et al.  Noninvasive measurement of abdominal aortic aneurysms in intact mice by a high-frequency ultrasound imaging system. , 2005, Ultrasound in medicine & biology.

[7]  Natesa G Pandian,et al.  Use of pulse wave and color flow Doppler echocardiography in mouse models of human disease. , 2002, Journal of the American Society of Echocardiography : official publication of the American Society of Echocardiography.

[8]  S. Kaul,et al.  Three-dimensional myocardial contrast echocardiography: validation of in vivo risk and infarct volumes. , 1997, Journal of the American College of Cardiology.

[9]  A R Jayaweera,et al.  Quantification of myocardial blood flow with ultrasound-induced destruction of microbubbles administered as a constant venous infusion. , 1998, Circulation.

[10]  S. Feinstein,et al.  Effect of intracoronary injections of sonicated microbubbles on left ventricular contractility. , 1987, The American journal of cardiology.

[11]  J. Ross,et al.  Akt induces enhanced myocardial contractility and cell size in vivo in transgenic mice , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[12]  M. Pfeffer,et al.  Myocardial Infarct Size and Ventricular Function in Rats , 1979, Circulation research.

[13]  E. Chérin,et al.  A new ultrasound instrument for in vivo microimaging of mice. , 2002, Ultrasound in medicine & biology.

[14]  J. Hossack,et al.  Observation of transient perfusion defects in a mouse heart using contrast ultrasound , 2003, IEEE Symposium on Ultrasonics, 2003.

[15]  S. Kaul.  Myocardial contrast echocardiography: basic principles. , 2001, Progress in cardiovascular diseases.

[16]  Z. Yang,et al.  Crucial role of endogenous interleukin-10 production in myocardial ischemia/reperfusion injury. , 2000, Circulation.

[17]  S. Kaul,et al.  Myocardial contrast echocardiography. , 2004, Circulation.

[18]  S. Kaul,et al.  Modeling the myocardial dilution curve of a pure intravascular indicator. , 1997, American journal of physiology. Heart and circulatory physiology.

[19]  R. Bolli,et al.  Demonstration of an early and a late phase of ischemic preconditioning in mice. , 1998, American journal of physiology. Heart and circulatory physiology.