Extending the limits of quantitative proteome profiling with data-independent acquisition

The “Profiling Standard Sample Set” consisted of eight sam-ples with a constant background (HEK-293 cells) and 12 non-human proteins spiked in three master mix groups. Master mix 1 and 2 were spiked in at small concentration changes on two concentration ranges. The master mix 3 was spiked in as a four fold dilution series. The lowest concentration was set to the value 1.