Multispectral microscopy and cell segmentation for analysis of thyroid fine needle aspiration cytology smears

This paper discusses the needs for automated tools to aid in the diagnosis of thyroid nodules based on analysis of fine needle aspiration cytology smears. While conventional practices rely on the analysis of grey scale or RGB color images, we present a multispectral microscopy system that uses thirty-one spectral bands for analysis. Discussed are methods and results for system calibration and cell delineation.

[1]  L. Hegedüs,et al.  The Thyroid Nodule , 2004 .

[2]  Shishir Shah,et al.  Comparative analysis of cell segmentation using absorption and color images in fine needle aspiration cytology , 2008, 2008 IEEE International Conference on Systems, Man and Cybernetics.

[3]  Elie E. Rebeiz,et al.  Diagnostic fluorescence spectroscopy of oral mucosa , 1995, Photonics West.

[4]  E. Mazzaferri,et al.  Management of a solitary thyroid nodule. , 1993, The New England journal of medicine.

[5]  Cao Feng,et al.  New Abnormal Cervical Cell Detection Method of Multi-Spectral Pap Smears , 2007 .

[6]  Harlan I. Firminger,et al.  Atlas of tumor pathology , 1954 .

[7]  Luc Vincent,et al.  Watersheds in Digital Spaces: An Efficient Algorithm Based on Immersion Simulations , 1991, IEEE Trans. Pattern Anal. Mach. Intell..

[8]  John P. Kerekes,et al.  Spectral imaging system analytical model for subpixel object detection , 2002, IEEE Trans. Geosci. Remote. Sens..

[9]  Hans Perrild,et al.  Diagnosis and treatment of the solitary thyroid nodule. Results of a European survey , 1999, Clinical endocrinology.

[10]  Olivier Lezoray,et al.  SUPERVISED AUTOMATIC HISTOGRAM CLUSTERING AND WATERSHED SEGMENTATION. APPLICATION TO MICROSCOPIC MEDICAL COLOR IMAGES , 2011 .

[11]  R. Leitner,et al.  On-Line Classification of Synthetic Polymers Using near Infrared Spectral Imaging , 2003 .

[12]  L. Hegedüs,et al.  Management of simple nodular goiter: current status and future perspectives. , 2003, Endocrine reviews.

[13]  L. Hegedüs,et al.  Management of the solitary thyroid nodule: results of a North American survey. , 2000, The Journal of clinical endocrinology and metabolism.

[14]  Jerome A. Feldman,et al.  Dynamic connections in neural networks , 1990, Biological Cybernetics.

[15]  James Thigpen,et al.  Photometric Calibration for Automated Multispectral Imaging of Biological Samples , 2006 .

[16]  Seung-Uk Yang,et al.  A high-resolution multi-spectral imaging system for small satellites , 2003 .

[17]  Pierre Soille,et al.  Morphological Image Analysis: Principles and Applications , 2003 .

[18]  A. Lawrence,et al.  The incidence of thyroid carcinoma in solitary cold nodules and in multinodular goiters. , 1986, Surgery.

[19]  K. Suen.  Guidelines of The Papanicolaou Society of Cytopathology for the examination of fine‐needle aspiration specimens from thyroid nodules: The Papanicolaou Society of Cytopathology Task Force on Standards of Practice , 1996, Diagnostic cytopathology.

[20]  P. Ladenson,et al.  Treatment guidelines for patients with thyroid nodules and well-differentiated thyroid cancer. American Thyroid Association. , 1996, Archives of internal medicine.

[21]  E. Mazzaferri,et al.  Thyroid cancer in thyroid nodules: finding a needle in the haystack. , 1992, The American journal of medicine.

[22]  Hubert Cardot,et al.  Cooperation of color pixel classification schemes and color watershed: a study for microscopic images , 2002, IEEE Trans. Image Process..

[23]  Education in Pathology.  Atlas of tumor pathology. Third series , 1991 .

[24]  J. Serpell,et al.  Management of the solitary thyroid nodule. , 2008, The oncologist.

[25]  Yasushi Hiraoka,et al.  Spectral imaging fluorescence microscopy , 2002, Genes to cells : devoted to molecular & cellular mechanisms.