Measuring blood oxygen saturation along a capillary vessel in human.

Measuring oxygen saturation in capillary vessels could provide valuable information on oxygen transport and tissue viability. Most spectroscopic measurement techniques, however, lack the spatial resolution to account for the small vessel dimensions within a scattering tissue and the steep gradients of oxygen saturation levels. Here, we developed a noninvasive technique for image-guided confocal measurement of the optical absorption spectrum from a small region that is comparable in size to the cross section of a single capillary vessel. A wide range of oxygen saturation levels were measured in a single capillary in a human volunteer, with blood deoxygenation rates of 7.1% per hundred microns. The technique could help in studying oxygen exchange dynamics in tissues and could play a key role in future clinical diagnosis and therapeutic applications that require localized functional tissue inspection.

[1]  Claude A Piantadosi,et al.  Automated measurement of blood flow velocity and direction and hemoglobin oxygen saturation in the rat lung using intravital microscopy. , 2013, American journal of physiology. Lung cellular and molecular physiology.

[2]  D. Schweitzer,et al.  In vivo measurement of the oxygen saturation of retinal vessels in healthy volunteers , 1999, IEEE Transactions on Biomedical Engineering.

[3]  Daniel J Buysse,et al.  Sleep-related breathing disorders in adults: recommendations for syndrome definition and measurement techniques in clinical research. The Report of an American Academy of Sleep Medicine Task Force. , 1999, Sleep.

[4]  S. Jacques.  Optical properties of biological tissues: a review , 2013, Physics in medicine and biology.

[5]  I. Zeng,et al.  Isolated nocturnal desaturation in COPD: prevalence and impact on quality of life and sleep , 2008, Thorax.

[6]  Yan Liu,et al.  Calibration-free quantification of absolute oxygen saturation based on the dynamics of photoacoustic signals. , 2013, Optics letters.

[7]  Kirk H. Shelley,et al.  Measuring venous oxygenation using the photoplethysmograph waveform , 2010, Journal of Clinical Monitoring and Computing.

[8]  Transcutaneous Regional Venous Oximetry: A Feasibility Study , 2011, Anesthesia and analgesia.

[9]  G. Millikan.  The Oximeter, an Instrument for Measuring Continuously the Oxygen Saturation of Arterial Blood in Man , 1942 .

[10]  D. V. Slyke,et al.  The determination of gases in blood and other solutions by vacuum extraction and manometric measurement. I. , 1924, The Journal of biological chemistry.

[11]  Lihong V. Wang,et al.  Photoacoustic tomography: principles and advances. , 2016, Electromagnetic waves.

[12]  Martina Meinke,et al.  Determination of optical properties of human blood in the spectral range 250 to 1100 nm using Monte Carlo simulations with hematocrit-dependent effective scattering phase functions. , 2006, Journal of biomedical optics.

[13]  D. Boas,et al.  Dendritic phosphorescent probes for oxygen imaging in biological systems. , 2009, ACS Applied Materials and Interfaces.

[14]  L. Dexter,et al.  Studies of congenital heart disease: technique of venous catheterization as a diagnostic procedure. , 1947, The Journal of clinical investigation.

[15]  Y. Shimada,et al.  Spectrophotometric monitoring of arterial oxygen saturation in the fingertip , 2006, Medical and Biological Engineering and Computing.

[16]  B. Reglin,et al.  An Imaging Spectroscopy Approach for Measurement of Oxygen Saturation and Hematocrit During Intravital Microscopy , 2007, Microcirculation.

[17]  Levkovich YuI,et al.  Blood flow velocity in capillaries of brain and muscles and its physiological significance. , 1981, Microvascular research.

[18]  M. Ducros,et al.  Simultaneous two-photon imaging of oxygen and blood flow in deep cerebral vessels , 2011, Nature Medicine.

[19]  P Altmeyer,et al.  Capillary blood cell velocity in human skin capillaries located perpendicularly to the skin surface: measured by a new laser Doppler anemometer. , 1996, Microvascular research.

[20]  J. Hickam,et al.  Studies of the Retinal Circulation in Man: Observations on Vessel Diameter, Arteriovenous Oxygen Difference, and Mean Circulation Time , 1966, Circulation.