Expression of epidermal growth factor receptors in four histologic cell types of lung cancer

Lung cancer tissues from 68 patients were examined for epidermal growth factor (EGF) receptor levels and EGF receptor gene copy numbers. Histologic cell types of these lung cancer tissues included squamous‐cell carcinoma (n = 30), adenocarcinoma (n = 28), large‐cell carcinoma (n = 4), and small‐cell carcinoma (n = 6). Tissues of squamous‐cell carcinoma exhibited exceptionally high 125I‐EGF binding activity, and those of small‐cell carcinoma showed no EGF binding activity. Southern blot hybridization analysis revealed EGF receptor gene amplification in the squamous‐cell carcinomas with high EGF binding activity. The EGF receptor levels in squamous‐cell carcinomas and adenocarcinomas were compared with their pathological staging grouping and pathological findings, including degree of differentiation, diameter of tumor, and lymph node metastasis. However, unlike previous reports on breast and bladder cancers, there was no obvious correlation between these pathological characteristics and the EGF receptor levels of lung cancer.

[1]  T. Nishikawa,et al.  Distribution of EGF receptor expressing and DNA replicating epidermal cells in psoriasis vulgaris and Bowen's disease , 1988, The British journal of dermatology.

[2]  D. Podolsky,et al.  Multiplicity of transforming growth factors in human malignant effusions. , 1988, Cancer research.

[3]  N. Shimizu,et al.  Stimulation by EGF of the growth of EGF receptor‐hyperproducing tumor cells in athymic mice , 1987, International journal of cancer.

[4]  J. Mendelsohn,et al.  Epidermal growth factor receptor expression in human lung carcinomas defined by a monoclonal antibody. , 1987, Journal of the National Cancer Institute.

[5]  I. Pastan,et al.  Molecular evidence for the lack of epidermal growth factor receptor gene expression in small cell lung carcinoma cells. , 1987, Cancer research.

[6]  N. Shimizu,et al.  High incidence of egf receptor hyperproduction in esophageal squamous‐cell carcinomas , 1987, International journal of cancer.

[7]  D. Goeddel,et al.  Synthesis of Messenger RNAs for Transforming Growth Factors α and β and the Epidermal Growth Factor Receptor by Human Tumors , 1987 .

[8]  Shu-sen Lin,et al.  Expression of epidermal growth factor receptors in human lung tumors , 1986, Cancer.

[9]  S. Sakiyama,et al.  Expression of epidermal growth factor receptor gene in cultured human lung cancer cells. , 1986, Japanese journal of cancer research : Gann.

[10]  N. Shimizu,et al.  Molecular mechanisms involved in increasing epidermal growth factor receptor levels on the cell surface. , 1986, Japanese journal of cancer research : Gann.

[11]  D. Appleton,et al.  Presence of epidermal growth factor receptor as an indicator of poor prognosis in patients with breast cancer. , 1985, Journal of clinical pathology.

[12]  I. Pastan,et al.  Hyperproduction and gene amplification of the epidermal growth factor receptor in squamous cell carcinomas. , 1985, Japanese journal of cancer research : Gann.

[13]  Y H Xu,et al.  Structure and localization of genes encoding aberrant and normal epidermal growth factor receptor RNAs from A431 human carcinoma cells , 1985, Molecular and cellular biology.

[14]  M. Bennett,et al.  EPIDERMAL-GROWTH-FACTOR RECEPTORS IN HUMAN BLADDER CANCER: COMPARISON OF INVASIVE AND SUPERFICIAL TUMOURS , 1985, The Lancet.

[15]  G. Sherbet,et al.  EPIDERMAL-GROWTH-FACTOR RECEPTORS AND OESTROGEN RECEPTORS IN HUMAN BREAST CANCER , 1985, The Lancet.

[16]  Y. S. Kim,et al.  Different responses to EGF in two human carcinoma cell lines, A431 and UCVA-1, possessing high numbers of EGF receptors , 1984, Molecular and Cellular Endocrinology.

[17]  J. Wittliff,et al.  Epidermal growth factor binding by breast tumor biopsies and relationship to estrogen receptor and progestin receptor levels. , 1984, Cancer research.

[18]  B. Ozanne,et al.  Human squamous cell lung cancers express increased epidermal growth factor receptors. , 1984, The Journal of clinical investigation.

[19]  N. Shimizu,et al.  Uptake of epidermal growth factor into a lysosomal enzyme‐deficient organelle: Correlation with cell's mitogenic response and evidence for ubiquitous existence in fibroblasts , 1984, Journal of cellular physiology.

[20]  T. Libermann,et al.  Expression of epidermal growth factor receptors in human brain tumors. , 1984, Cancer research.

[21]  N. Yanaihara,et al.  Production and molecular size heterogeneity of immunoreactive gastrin-releasing peptide in fetal and adult lungs and primary lung tumors. , 1983, Cancer research.

[22]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[23]  N. Nomura,et al.  High incidence of amplification of the epidermal growth factor receptor gene in human squamous carcinoma cell lines. , 1986, Cancer research.

[24]  D. Bostwick,et al.  Gastrin releasing peptide is a selective mitogen for small cell lung carcinoma in vitro. , 1985, The Journal of clinical investigation.

[25]  G. Sherbet,et al.  EPIDERMAL-GROWTH-FACTOR RECEPTORS AND ESTROGEN-RECEPTORS IN HUMAN-BREAST CANCER , 1985 .

[26]  A. Harris.  Letter: Vitamin-C-induced hyperoxaluria. , 1976, Lancet.