论文信息 - Application of the Thermodynamic Parameters of DNA Stability Prediction to Double-Helix Formation of Deoxyribooligonucleotides

Application of the Thermodynamic Parameters of DNA Stability Prediction to Double-Helix Formation of Deoxyribooligonucleotides

Abstract The energetics of a double helix formation of deoxyribooligonucleotides has been studied by measuring optical melting curves and calculating thermodynamic values based on the nearest neighbor model and parameters. The results show that, though the model is valid, the values of the nearest-neighbor thermodynamic parameters reported previously should be improved for some base pairs in order to predict more precisely the stability of the double helix of the oligomers by the parameters.

[1] C. E. Longfellow,et al. Polymer-supported RNA synthesis and its application to test the nearest-neighbor model for duplex stability. , 1986, Biochemistry.

[2] D. Turner,et al. RNA structure prediction. , 1988, Annual review of biophysics and biophysical chemistry.

[3] D. Turner,et al. Improved free-energy parameters for predictions of RNA duplex stability. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[4] I. Tinoco,et al. Estimation of Secondary Structure in Ribonucleic Acids , 1971, Nature.

[5] J. Shaffer,et al. Hybridization of synthetic oligodeoxyribonucleotides to ΦX 174 DNA: the effect of single base pair mismatch , 1979 .

[6] H. Blöcker,et al. Predicting DNA duplex stability from the base sequence. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[7] Naoki Sugimoto,et al. Double-Helix Melting of Octamers of Deoxyriboadenylic and Deoxyribothymidylic Acids in the Presence of Ethidium , 1991 .

[8] G. Fasman,et al. Handbook of biochemistry and molecular biology. Nucleic acids - v. 1 - 3. ed. , 1975 .

[9] T. Cech,et al. Catalysis of RNA cleavage by the Tetrahymena thermophila ribozyme. 1. Kinetic description of the reaction of an RNA substrate complementary to the active site. , 1990, Biochemistry.