Klinik örneklerden izole edilen Pseudomonas aeruginosa suşlarinda antibiyotik direnci Antimicrobial resistance of Pseudomonas aeruginosa strains isolated from clinical specimens

Amac: Pseudomonas aeruginosa ozellikle hastanede yatan hastalarda ve bagisiklik sistemi zayiflamis bireylerde ciddi ve hayati tehdit edici enfeksiyonlarin iyi bilinen bir etkenidir. Bu calismanin amaci P. aeruginosa suslarinda antibiyotik duyarlilik profilinin belirlenmesidir. Yontem: Eylul 2011 - Nisan 2013 arasinda hastanemize basvuran hastalarin cesitli klinik orneklerinden izole edilen toplam 137 P. aeruginosa susu calismaya dahil edildi. Suslarin identifikasyonu ve antibiyotik duyarlilik profilleri VITEK 2 (bioMerieux, Fransa) otomatize sistemi ile belirlendi. Bulgular: Izolatlarda direnc oranlari amikasine %43, gentamisine %38, seftazidime %42, sefepime %40, sefoperazon-sulbaktama %44, siprofloksasine %47, levofloksasine %47, piperasiline %72, piperasilin- tazobaktama %71, imipeneme %37, meropeneme %37, doripeneme %41, kolistine %7 ve netilmisine %28 olarak belirlendi. Kolistin P. aeruginosa suslarina en etkili antimikrobiyal ajan olarak saptandi. Yogun bakim izolatlarinda direnc oranlari klinik izolatlarina gore daha direncli bulundu. Sonuc: Hastanemizde izole edilen P. aeruginosa suslarinda gozlenen yuksek antibiyotik direnci ve coklu-direnc ozellikleri diger calismalara benzer bulundu. P. aeruginosa izolatlarinda yuksek antibiyoik direnc oranlarinin saptanmis olmasi hastanede antibiyotik kullanimi ile ilgili etkili politikalar gelistirilerek uygun bir enfeksiyon kontrol programi uygulanmasini gerektirmektedir. Objective: Pseudomonas aeruginosa is a well-known cause of severe and potentially life-threatening infections especially in hospitalized patients and individuals with weakened immune system due to various reasons. The aim of this study was to determine antibiotic susceptibility profile of P. aeruginosa strains. Method: A total of 137 P. aeruginosa strains isolated from the clinical samples of patients applied to our hospital between the September 2011 - April 2013, were included in the study. The identification and antibiotic susceptibility profiles were investigated by automated commercial system VITEK 2 (bioMerieux, France). Results: The resistance rates for amikacin, gentamycin, ceftazidime, cefepime, cefoperazon-sulbactam, ciprofloxacin, levofloxacin, piperacillin, piperacillin- tazobactam, imipenem, meropenem, doripenem, colistin and netilmicin were 43%, 38%, 42%, 40%, 44%, 47%, 47%, 72%, 71%, 37%, 37%, 41%, 7%, 28%, respectively. Colistin was found to be the most susceptible agents against P. aeruginosa strains.The resistance rates were higher among the strains isolated from the intensive care unit. Conclusion: High antimicrobial resistance and multi- resistance characteristics is observed in P. aeruginosa strains isolated from our hospital as concordant with the other studies. Detection of high resistance against antibiotics in isolates of P. aeruginosa requires implementation of the infection control program and the development of effective policy for rational use of antibiotics.