Proteomic analysis of the seeds of transgenic rice lines and the corresponding nongenetically modified isogenic variety.

BACKGROUND An isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis was employed to study the seeds of two genetically modified (GM) rice lines T2A-1 and T1C-19, and their nontransgenic isogenic variety MH63, to investigate the unintended effects of genetic modification. RESULTS A total of 3398 proteins were quantitatively identified. Seventy-seven differentially abundant proteins (DAPs) were identified in the T2A-1/MH63 comparison, and 70 and 7 of these DAPs were upregulated and downregulated, respectively. A pathway enrichment analysis showed that most of these DAPs participated in metabolic pathways and protein processing in endoplasmic reticulum and were ribosome components. One hundred eighty-one DAPs were identified from the T1C-19/MH63 comparison, and these included 115 upregulated proteins and 66 downregulated proteins. The subsequent pathway enrichment analysis showed that these DAPs mainly participated in protein processing in endoplasmic reticulum and carbon fixation in photosynthetic organisms and were ribosome components. None of these DAPs were identified as new unintended toxins or allergens, and only changes in abundance were detected. Fifty-four co-DAPs were identified in the seeds of two GM rice lines, and protein-protein interaction (PPI) analysis of these co-DAPs demonstrated that some interacting proteins were involved in protein processing in endoplasmic reticulum and metabolic pathways, whereas others were identified as ribosome components. Representative co-DAPs and proteins related to nutrients were analyzed by qRT-PCR to determine their transcriptional levels. CONCLUSION The results suggested that the seed proteomic profiles of two GM rice lines studied were not substantially altered from those of their natural isogenic control. This article is protected by copyright. All rights reserved.

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