Development of a Hypersensitive Detection Method for Human Parvovirus B19 DNA

ABSTRACT A new detection method for human parvovirus B19 DNA was established using PCR coupled with a hybridization protection assay. The amplified product was detected using acridinium ester-labeled DNA probes. By this method, a few copies of B19 DNA were detected in human serum albumin.

[1]  Shiro Iino,et al.  Quantitative Detection of Hepatitis B Virus by Transcription-Mediated Amplification and Hybridization Protection Assay , 1999, Journal of Clinical Microbiology.

[2]  K. Matsumoto,et al.  Screening of Blood Donors for Human Parvovirus B19 and Characterization of the Results , 1999, Vox Sanguinis.

[3]  P. Hammond,et al.  Detection of all single-base mismatches in solution by chemiluminescence. , 1996, Nucleic acids research.

[4]  L. Anderson,et al.  Genetic diversity of human parvovirus B19: sequence analysis of the VP1/VP2 gene from multiple isolates. , 1996, The Journal of general virology.

[5]  S. Modrow,et al.  Sequence variability among different parvovirus B19 isolates. , 1996, The Journal of general virology.

[6]  J. Saldanha,et al.  Detection of human parvovirus B19 DNA in plasma pools and blood products derived from these pools: implications for efficiency and consistency of removal of B19 DNA during manufacture , 1996, British journal of haematology.

[7]  Y. Yoto,et al.  Large‐scale screening for human parvovirus B19 DNA in clinical specimens by dot blot hybridization and polymerase chain reaction , 1995, Journal of medical virology.

[8]  K. Okochi,et al.  Screening of blood donors for human parvovirus B19 , 1995, The Lancet.

[9]  K. Umene,et al.  A new genome type of human parvovirus B19 present in sera of patients with encephalopathy. , 1995, The Journal of general virology.

[10]  Y. Yamakawa,et al.  Detection of human parvovirus B19 DNA by nested polymerase chain reaction. , 1995, Obstetrics and gynecology.

[11]  S. Girotti,et al.  Chemiluminescence dot blot hybridization assay for detection of B19 parvovirus DNA in human sera , 1991, Journal of clinical microbiology.

[12]  D. Gibellini,et al.  Rapid screening for B19 parvovirus DNA in clinical specimens with a digoxigenin-labeled DNA hybridization probe , 1990, Journal of clinical microbiology.

[13]  S. Adler,et al.  Detection of human parvovirus B19 DNA by using the polymerase chain reaction , 1990, Journal of clinical microbiology.

[14]  P. Hammond,et al.  Assay formats involving acridinium-ester-labeled DNA probes. , 1989, Clinical chemistry.

[15]  J. Clewley,et al.  SYMPTOMATIC PARVOVIRUS B19 INFECTION AND HEAT-TREATED FACTOR IX CONCENTRATE , 1989, The Lancet.

[16]  J. Clewley,et al.  Dot blot hybridization assay of B19 virus DNA in clinical specimens , 1989, Journal of clinical microbiology.

[17]  F. M. van de Rijke,et al.  Rapid detection of human parvovirus B19 DNA by dot-hybridization and the polymerase chain reaction. , 1989, Journal of virological methods.

[18]  C. Astell,et al.  In vitro identification of a B19 parvovirus promoter. , 1987, Virology.

[19]  P. Tattersall,et al.  Nucleotide sequence and genome organization of human parvovirus B19 isolated from the serum of a child during aplastic crisis , 1986, Journal of virology.

[20]  K. Mullis,et al.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. , 1985, Science.

[21]  Henry A. Erlich,et al.  Enzymatic amplification of ?-globin genomic sequences and restriction site analysis for diagnosis of , 1985 .