Cryptosporidium identification in HIV-Infected Humans. Experience from Kinshasa, the Democratic Republic of Congo

Cryptosporidium is an important protozoan parasite affecting HIV/AIDS patients. To determine the prevalence and the species of Cryptosporidium spp by developing a rapid and efficient real-time PCR-RFLP test. A cross-sectional study was conducted among HIV-infected adults from Kinshasa, the Democratic Republic of Congo. Stool specimens were examined by microscopic evaluation and real-time PCR-RFLP. Out of 242 HIV-infected adults, 10 (4.1%) cases of Cryptosporidium were identified by microscopic examination. Using PCR-RFLP, the prevalence of Cryptosporidium spp was 5.4% (n = 13). All the 13 cases of Cryptosporidium spp had the stage of AIDS of HIV infection. Extracted DNA was amplified by nested PCR targeting a 1030-bp fragment of the 18s RNA gene. RFLP analysis identified one C. parvum, four C. hominis and one non determined Cryptosporidium. The capacity to detect C. parvum, C. hominis and non-determined Cryptosporidium was present among our HIV-infected patients.

[1]  E. Kendjo,et al.  Epidemiology, clinical, immune, and molecular profiles of microsporidiosis and cryptosporidiosis among HIV/AIDS patients , 2012, International journal of general medicine.

[2]  Qing He,et al.  Cryptosporidiosis-an overview , 2011, Journal of biomedical research.

[3]  U. Unicef,et al.  Global HIV/AIDS response: epidemic update and health sector progress towards universal access: progress report 2011. , 2011 .

[4]  J. Breton,et al.  Intestinal parasites infections in hospitalized AIDS patients in Kinshasa, Democratic Republic of Congo. , 2010, Parasite.

[5]  F. Derouin,et al.  Identification of cryptosporidium species infecting humans in Tunisia. , 2008, The American journal of tropical medicine and hygiene.

[6]  C. Hennequin,et al.  [Prevalence of opportunistic digestive parasitic infections in Kinshasa, Democratic Republic of Congo. Results of a preliminary study in 50 AIDS patients]. , 2007, Medecine tropicale : revue du Corps de sante colonial.

[7]  Anthony K. Waruru,et al.  Cryptosporidiosis: prevalence, genotype analysis, and symptoms associated with infections in children in Kenya. , 2006, The American journal of tropical medicine and hygiene.

[8]  R. Porcher,et al.  Experimental study of the effects of probiotics on Cryptosporidium parvum infection in neonatal rats , 2006, Parasitology Research.

[9]  F. Derouin,et al.  Detection of Cryptosporidium and Identification to the Species Level by Nested PCR and Restriction Fragment Length Polymorphism , 2005, Journal of Clinical Microbiology.

[10]  E. Guillot,et al.  Study of 18S rRNA and rDNA stability by real-time RT-PCR in heat-inactivated Cryptosporidium parvum oocysts. , 2003, FEMS microbiology letters.

[11]  R. Porcher,et al.  Development of a real-time polymerase-chain-reaction assay for quantitative detection of Enterocytozoon bieneusi DNA in stool specimens from immunocompromised patients with intestinal microsporidiosis. , 2003, The Journal of infectious diseases.

[12]  J. P. Davis,et al.  Costs of Illness in the 1993 Waterborne Cryptosporidium Outbreak, Milwaukee, Wisconsin , 2003, Emerging infectious diseases.

[13]  B. Gazzard,et al.  Eradication of Cryptosporidia and Microsporidia Following Successful Antiretroviral Therapy , 2000, Journal of acquired immune deficiency syndromes.

[14]  M. Bejaoui,et al.  [Role of cryptosporidia and microsporidia in diarrhea in immunocompromised patients]. , 1999, La Tunisie medicale.

[15]  F. Carrat,et al.  Factors of weight loss in patients with HIV and chronic diarrhea. , 1998, Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association.

[16]  J. Paul,et al.  Chronic diarrhoea among HIV-infected adult patients in Nairobi, Kenya , 1998, Journal of Infection.

[17]  R. Cordell,et al.  Impact of a massive waterborne cryptosporidiosis outbreak on child care facilities in metropolitan Milwaukee, Wisconsin. , 1997, The Pediatric infectious disease journal.

[18]  Alimuddin Zumla,et al.  Gut parasites in HIV-seropositive Zambian adults with diarrhoea. , 1994, East African medical journal.

[19]  B. Mengesha.  Cryptosporidiosis among medical patients with the acquired immunodeficiency syndrome in Tikur Anbessa Teaching Hospital, Ethiopia. , 1994, East African medical journal.

[20]  O. Ndir,et al.  [Prevalence of Cryptosporidium sp and Isospora belli in patients with acquired immunodeficiency syndrome (AIDS) in Dakar (Senegal]. , 1994, Dakar medical.

[21]  B. Thom,et al.  SCREENING FOR CRYPTOSPORIDIUM IN STOOLS , 1984, The Lancet.