Effect of Carbon/Nitrogen Ratio, Temperature, and Inoculum Source on Hydrogen Production from Dark Codigestion of Fruit Peels and Sewage Sludge

This paper studies the use of fruit peel biomass and waste sludge from municipal wastewater treatment plants in the metropolitan area of Monterrey, Mexico as an alternative way of generating renewable energy. Using a Plackett–Burman experimental design, we investigated the effects of temperature, inoculum source, and the C/N (Carbon/Nitrogen) ratio on dark fermentation (DF). The results indicate that it is possible to produce hydrogen using fruit peels codigested with sewage sludge. By adjusting the C/N ratio in response to the physicochemical characterization of the substrates, it was revealed that the quantities of carbohydrates and nitrogen were sufficient for the occurrence of the fermentation process with biogas production greater than 2221 ± 5.8 mL L−1Reactor and hydrogen selectivity of 23% (366 ± 1 mL H2·L−1Reactor) at the central point. The kinetic parameters (Hmax= 86.6 mL·L−1, Rm = 2.6 mL L−1 h−1, and λ = 1.95 h) were calculated using the modified Gompertz model. The quantification of soluble metabolites, such as acetic acid (3600 mg L−1) and ethyl alcohol (3.4 ± 0.25% v/v), confirmed the presence of acetogenesis in the generation of hydrogen.

[1]  Clay Aldás,et al.  Municipal solid waste as a valuable renewable energy resource: a worldwide opportunity of energy recovery by using Waste-To-Energy Technologies , 2017 .

[2]  I. Chairez,et al.  Biohydrogen Production Based on the Evaluation of Kinetic Parameters of a Mixed Microbial Culture Using Glucose and Fruit–Vegetable Waste as Feedstocks , 2013, Applied Biochemistry and Biotechnology.

[3]  T. Sreekrishnan,et al.  Effect of Heat Pretreated Consortia on Fermentative Biohydrogen Production from Vegetable Waste , 2013 .

[4]  H. Argun,et al.  Bio-hydrogen production from waste peach pulp by dark fermentation: Effect of inoculum addition , 2017 .

[5]  G. Buitrón,et al.  Comparison of hydrogen-producing bacterial communities adapted in continuous and discontinuous reactors , 2014 .

[6]  E. León‐Becerril,et al.  A novel biohydrogen production process: Co-digestion of vinasse and Nejayote as complex raw substrates using a robust inoculum , 2017 .

[7]  A. Panico,et al.  The effect of bacterial and archaeal populations on anaerobic process fed with mozzarella cheese whey and buttermilk. , 2018, Journal of environmental management.

[8]  Carlos Escamilla-Alvarado,et al.  Biohydrogen, biomethane and bioelectricity as crucial components of biorefinery of organic wastes: A review , 2014, Waste management & research : the journal of the International Solid Wastes and Public Cleansing Association, ISWA.

[9]  Hang-Sik Shin,et al.  Experience of a pilot-scale hydrogen-producing anaerobic sequencing batch reactor (ASBR) treating food waste , 2010 .

[10]  Martin Zerta,et al.  Alternative World Energy Outlook (AWEO) and the role of hydrogen in a changing energy landscape , 2008 .

[11]  F. Smith,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .

[12]  Haris Nalakath Abubackar,et al.  Biohydrogen production from autoclaved fruit and vegetable wastes by dry fermentation under thermophilic condition , 2019, International Journal of Hydrogen Energy.

[13]  Fabián Fernández-Luqueño,et al.  Renewable energy sources for electricity generation in Mexico: A review , 2017 .

[14]  D. Ahmad,et al.  Biohydrogen generation from jackfruit peel using anaerobic contact filter , 2006 .

[15]  K. Valta,et al.  Current Treatment Technologies of Cheese Whey and Wastewater by Greek Cheese Manufacturing Units and Potential Valorisation Opportunities , 2017 .

[16]  Quevedo Amaya,et al.  Caracterización fisiológica y bioquímica de cuatro genotipos de algodón (Gossypium hirsutum L.) en condiciones de déficit hídrico , 2020 .

[17]  Gerasimos Lyberatos,et al.  Biohydrogen Production from Biomass and Wastes via Dark Fermentation: A Review , 2010 .

[18]  P. Hallenbeck.  BIOHYDROGEN-THE MICROBIOLOGICAL PRODUCTION OF HYDROGEN FUEL , 2011 .

[19]  B. Zhang,et al.  Landfill Risk Assessment on Groundwater Based on Vulnerability and Pollution Index , 2016, Water Resources Management.

[20]  P. Parameswaran,et al.  Anaerobic digestion and co-digestion processes of vegetable and fruit residues: process and microbial ecology. , 2011, Bioresource technology.

[21]  Hang-Sik Shin,et al.  FEASIBILITY OF BIOHYDROGEN PRODUCTION BY ANAEROBIC CO-DIGESTION OF FOOD WASTE AND SEWAGE SLUDGE , 2004 .

[22]  Piet N.L. Lens,et al.  A review on dark fermentative biohydrogen production from organic biomass: Process parameters and use of by-products , 2015 .

[23]  M. Hamdi,et al.  Enhancement of fermentative hydrogen production by Thermotoga maritima through hyperthermophilic anaerobic co-digestion of fruit-vegetable and fish wastes , 2018, International Journal of Hydrogen Energy.

[24]  A. Ganguly,et al.  Process kinetic studies of biohydrogen production by co-fermentation of fruit-vegetable wastes and cottage cheese whey , 2018, Energy for Sustainable Development.

[25]  A Polettini,et al.  A review of dark fermentative hydrogen production from biodegradable municipal waste fractions. , 2013, Waste management.

[26]  Shubin Wu,et al.  Hydrogen-methane production from pulp & paper sludge and food waste by mesophilic–thermophilic anaerobic co-digestion , 2013 .

[27]  Germán Buitrón,et al.  Biohydrogen production from Tequila vinasses in an anaerobic sequencing batch reactor: effect of initial substrate concentration, temperature and hydraulic retention time. , 2010, Bioresource technology.

[28]  H. Hou,et al.  Optimization of Process Parameters for Directly Converting Raw Corn Stalk to Biohydrogen by Clostridium sp. FZ11 without Substrate Pretreatment , 2016 .

[29]  Ange Nzihou.  Toward the Valorization of Waste and Biomass , 2010 .

[30]  H. Pinheiro,et al.  Combining biotechnology with circular bioeconomy: From poultry, swine, cattle, brewery, dairy and urban wastewaters to biohydrogen , 2018, Environmental Research.

[31]  M. Taherzadeh,et al.  Evaluation of Fermentative Hydrogen Production from Single and Mixed Fruit Wastes , 2015 .

[32]  Yuansong Wei,et al.  Enhanced biohydrogen production from sewage sludge with alkaline pretreatment. , 2004, Environmental science & technology.

[33]  T. Tan,et al.  Biohydrogen Production by Dark Fermentation of Starch Using Mixed Bacterial Cultures of Bacillus sp and Brevumdimonas sp. , 2012 .

[34]  I. Valdez‐Vazquez,et al.  Characterization of a Lignocellulolytic Consortium and Methane Production from Untreated Wheat Straw: Dependence on Nitrogen and Phosphorous Content , 2016 .

[35]  Isaac Chairez,et al.  Selective adaptation of an anaerobic microbial community: Biohydrogen production by co-digestion of cheese whey and vegetables fruit waste , 2014 .

[36]  A. Mudhoo,et al.  Co–digestion of coffee residues and sugarcane vinasse for biohythane generation , 2018 .

[37]  Gyoo Yeol Jung,et al.  Current status of the metabolic engineering of microorganisms for biohydrogen production. , 2011, Bioresource technology.

[38]  Chiu-Yue Lin,et al.  Enhanced biohydrogen production from beverage wastewater: process performance during various hydraulic retention times and their microbial insights , 2016 .

[39]  S. Tan,et al.  Genetic Characterization of Wild Stocks of Prawns M. rosenbergii using Random Amplified Polymorphic DNA Markers , 2008 .

[40]  Sureewan Sittijunda,et al.  Co-Digestion of Napier Grass and Its Silage with Cow Dung for Bio-Hydrogen and Methane Production by Two-Stage Anaerobic Digestion Process , 2017 .

[41]  Piet N.L. Lens,et al.  Biohydrogen production from food waste by coupling semi-continuous dark-photofermentation and residue post-treatment to anaerobic digestion: A synergy for energy recovery , 2015 .

[42]  A. Pozos,et al.  Zn-Ge oxynitride based nano-photocatalyst for hydrogen production under visible light , 2016 .

[43]  S. O-thong,et al.  Effect of initial pH, nutrients and temperature on hydrogen production from palm oil mill effluent using thermotolerant consortia and corresponding microbial communities , 2012 .

[44]  D. Das,et al.  Comparative evaluation of the hydrogen production by mixed consortium, synthetic co-culture and pure culture using distillery effluent. , 2015, Bioresource technology.

[45]  D. Ahmad,et al.  Bio-hydrogen generation from mixed fruit peel waste using anaerobic contact filter , 2007 .

[46]  G. Cappai,et al.  Biohydrogen Production from Food Waste: Influence of the Inoculum-To-Substrate Ratio , 2018, Sustainability.

[47]  Tiago Pinto,et al.  Production and storage of biohydrogen during sequential batch fermentation of Spirogyra hydrolyzate by Clostridium butyricum , 2015 .

[48]  Ramakrishnan Nagasundara Ramanan,et al.  Improvement of Biohydrogen Production through Combined Reuses of Palm Oil Mill Effluent Together with Pulp and Paper Mill Effluent in Photofermentation , 2015 .

[49]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.