Induction of Fimbriated Vibrio cholerae O139

ABSTRACT Several fimbriated phases of Vibrio cholerae O139 strains were selectively induced and compared immunologically and biochemically with those of V. cholerae O1. Fimbrial antigens were detected on the surfaces of vibrio cells colonizing the epithelial cells of a rabbit small intestine. Convalescent-phase sera from six individuals infected with V. cholerae O139 revealed the development of antibody against the fimbrillin. These findings suggest that the fimbriae of V. cholerae O1 and O139 are expressed in vivo during infection and that consideration must be given to the use of fimbrial antigens as components of vaccines against cholera.

[1]  A. Takade,et al.  Morphological Features of a Filamentous Phage of Vibrio cholerae O139 Bengal , 1997, Microbiology and immunology.

[2]  T. Hirayama,et al.  Characterization of filamentous phages of Vibrio cholerae O139 and O1. , 1997, FEMS microbiology letters.

[3]  J. Morris,et al.  Vibrio cholerae O1 can assume a chlorine-resistant rugose survival form that is virulent for humans. , 1996, The Journal of infectious diseases.

[4]  J. Holmgren,et al.  Relative significance of mannose-sensitive hemagglutinin and toxin-coregulated pili in colonization of infant mice by Vibrio cholerae El Tor , 1996, Infection and immunity.

[5]  R. Taylor,et al.  Toxin-coregulated pilus, but not mannose-sensitive hemagglutinin, is required for colonization by Vibrio cholerae O1 El Tor biotype and O139 strains , 1996, Infection and immunity.

[6]  Matthew K. Waldor,et al.  Lysogenic Conversion by a Filamentous Phage Encoding Cholera Toxin , 1996, Science.

[7]  Y. Ichinose,et al.  Immunogenicity of Vibrio cholerae O1 Fimbriae in Animal and Human Cholera , 1993, Microbiology and immunology.

[8]  H. Towbin,et al.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. 1979. , 1992, Biotechnology.

[9]  Y. Ichinose,et al.  Selective Induction of Fimbriate Vibrio cholerae O1 , 1991 .

[10]  Y. Ichinose,et al.  Purification and Characterization of Fimbriae from Fimbriate Vibrio cholerae O1 Strain Bgd17 , 1991 .

[11]  M. Levine,et al.  Immunogenicity of Vibrio cholerae O1 toxin-coregulated pili in experimental and clinical cholera , 1991, Infection and immunity.

[12]  Koichiro Yamamoto,et al.  Culture Conditions for Stimulating Cholera Toxin Production by Vibrio cholerae O1 El Tor , 1986, Microbiology and immunology.

[13]  H. Towbin,et al.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[14]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[15]  S. De,et al.  An experimental study of the mechanism of action of Vibriod cholerae on the intestinal mucous membrane. , 1953, The Journal of pathology and bacteriology.