Expression of the PR-b1″ Gene in Roots of Two Nicotiana Species and their Amphidiploid Hybrid Infected with Virulent and Avirulent Races of Chalara Elegans

We have previously reported the production of the three main groups of PR-protein in roots of a moderately resistant Nicotiana tabacum cv Xanthi nc. infected by the black root rot fungus Chalara elegans. In this work we have investigated changes in PR-bl gene expression in roots of two Nicotiana species, N. debneyi, N. glutinosa and their amphidiploid hybrid N. glutinosa X N. debneyi in response to the infection by two isolates of C. elegans, 85-26 and 84-1 which are respectively avirulent and virulent races. N. debneyi is highly resistant toward C. elegans, its resistance has been reported to be of monogenic inheritance, while N. glutinosa is a susceptible one. The amphidiploid hybrid N. glutinosa X N. debneyi is also highly resistant to C. elegans. It has been shown that PR-b1″ is the only PR-1 protein produced by these plants and that it is expressed constitutively in the hybrid. The PR-b1″ is induced in either of the two parent plants only after the hypersensitive reaction to viruses infection or chemical treatments. We report here a correlation between the infection rate of Nicotiana roots by the pathogenic fungus C. elegans and the relative level of PR-b1″ gene expression in the two parent plants. Irrespective of whether the plant is susceptible or resistant and whether the fungal race is virulent or avirulent, the results obtained showed that, low infection rates induce low levels of PR-b1″ mRNA as seen in N. debneyi infected with avirulent race 85-26, and high infection rates results in high production of PR-b1″ mRNA as shown by N. debneyi inoculated with virulent race 84-1 and N. glutinosa interacting with either avirulent (85-26) or virulent (84-1) races.

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