Microstructure-based techniques for single-cell manipulation and analysis

Abstract Over the past few years, manipulating and analyzing methods based on single-cell level have become frequently adopted to conduct the cell heterogeneity study (e.g., differentiation of stem cells, tumor cell heterogeneity). Traditional single-cell analysis techniques exhibit high processing complexity and time-consuming characteristic, and require expensive equipment, considerably limiting their applications in cellular heterogeneity study. Microfluidics-based systems to conduct single-cell study have appeared to be powerful methods as fueled with the advancement of microfluidics techniques. This paper reviews microstructure-based methods for single-cell manipulation and analysis. The methods based on microvalve for single-cell manipulation are also discussed in this paper. Lastly, the challenges required to be addressed in the future are highlighted.

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