Biodegradation of Bonny light crude oil by Bacillus sp. and Pseudomonas sp.

Abstract The effect of temperature, pH, nitrogen, and phosphorus concentrations on the biodegradation of Bonny light crude oil by Bacillus sp and Pseudomonas sp was studied. Results obtained revealed that 4 g/L of nitrogen and 3 g/L of phosphorus supported the highest level of crude oil degradation by Bacillus sp, while 3.5 g/L of nitrogen and 3 g/L of phosphorus was best for Pseudomonas sp. Temperature of 36 °C supported the highest level of crude oil degradation by the two organisms. The two organisms were, however, observed to be able to degrade crude oil efficiently over a wide range of temperature (20 ° to 44 °C). The pH of 7.4 was optimum for crude oil degradation by Bacillus sp whereas that of Pseudomonas sp was pH 7.0. Crude oil degradation by the two organisms under the conditions provided in this study indicates that the organisms are suitable candidates for application in oil spill clean up of the Nigerian terrestrial ecosystem.