Sites of specific B cell activation in primary and secondary responses to T cell‐dependent and T cell‐independent antigens

Techniques which identify hapten‐specific B cells in tissues have been used to determine the sites of B cell activation in rat spleens in response to T cell‐dependent (TD) antigens and T cell‐independent type‐1 (TI‐1) antigens. Surface‐associated hapten binding by specific memory B cells and. B blasts was distinguished from the strong cytoplasmic hapten binding by specific plasma cells and plasmablasts. Blast cells in S phase were identified in tissue sections by staining cells which had been pulse labeled in vivo with 5‐bromo‐2′‐deoxyuridine.

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