Phenotype/genotype associations for yield and salt tolerance in a barley mapping population segregating for two dwarfing genes.

Barley traits related to salt tolerance are mapped in a population segregating for a dwarfing gene associated with salt tolerance. Twelve quantitative trait loci (QTLs) were detected for seven seedling traits in doubled haploids from the spring barley cross Derkado x B83-12/21/5 when given saline treatment in hydroponics. The location of QTLs for seedling growth stage (leaf appearance rate), stem weight prior to elongation, and tiller number are reported for the first time. In addition, four QTLs were found for the mature plant traits grain nitrogen and plot yield. In total, seven QTLs are co-located with the dwarfing genes sdw1, on chromosome 3H, and ari-e.GP, on chromosome 5H, including seedling leaf response (SGa) to gibberellic acid (GA(3)). QTLs controlling the growth of leaves (GS2) on chromosomes 2H and 3H and emergence of tillers (TN2) and grain yield were independent of the dwarfing genes. Field trials were grown in eastern Scotland and England to estimate yield and grain composition. A genetic map was used to compare the positions of QTLs for seedling traits with the location of QTLs for the mature plant traits. The results are discussed in relation to the study of barley physiology and the location of genes for dwarf habit and responses to GA.

[1]  J. Doe.  Sand and Water Culture Methods Used in the Study of Plant Nutrition , 1953, Soil Science Society of America Journal.

[2]  J. W. Morrison.  Barley Genetics I , 1965 .

[3]  E. Hewitt.  Sand and Water Culture Methods Used in the Study of Plant Nutrition , 1966 .

[4]  Kenneth Mather,et al.  Biometrical genetics , 1972, Heredity.

[5]  R. Ellis,et al.  A comparison of spring barley grown in England and in Scotland. 1. Shoot apex development , 1980, The Journal of Agricultural Science.

[6]  R. Munns,et al.  Mechanisms of salt tolerance in nonhalophytes. , 1980 .

[7]  Graham D. Farquhar,et al.  On the Relationship Between Carbon Isotope Discrimination and the Intercellular Carbon Dioxide Concentration in Leaves , 1982 .

[8]  W. Powell,et al.  The chromosomal location of the dwarfing gene present in the spring barley variety golden promise , 1984, Heredity.

[9]  S. Kanemaki,et al.  A Theory for the , 1986 .

[10]  T. E. Miller,et al.  Chromosome location of genes controlling tolerance to salt (NaCl) and vigour in Hordeum vulgare and H. chilense , 1990, Heredity.

[11]  W. Powell,et al.  The effects of major genes on quantitatively varying characters in barley. 4. The GPert and denso loci and quality characters , 1991, Heredity.

[12]  B. Forster.  Genetic engineering for stress tolerance in the Triticeae , 1992 .

[13]  E. Acevedo.  Potential of Carbon Isotope Discrimination as a Selection Criterion in Barley Breeding , 1993 .

[14]  Graham D. Farquhar,et al.  Stable isotopes and plant carbon-water relations. , 1993 .

[15]  C. Scrimgeour,et al.  Effects of the ecto‐ and VA‐mycorrhizal fungi Hydnagium carneum and Glomus clarum on the δ15N and δ13C values of Eucalyptus globulus and Ricinus communis , 1993 .

[16]  E. Nevo,et al.  Chromosome 4 controls potential water use efficiency (δ13C) in barley , 1994 .

[17]  E. Epstein.  The anomaly of silicon in plant biology. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[18]  Nicholas A. Tinker,et al.  MQTL: software for simplified composite interval mapping of QTL in multiple environments. , 1995 .

[19]  W. Powell,et al.  Polymorphism revealed by simple sequence repeats , 1996 .

[20]  J. Franckowiak.  REVISED LINKAGE MAPS FOR MORPHOLOGICAL MARKERS IN BARLEY, HORDEUM VULGARE , 1996 .

[21]  Bronwyn J. Barkla,et al.  PHYSIOLOGY OF ION TRANSPORT ACROSS THE TONOPLAST OF HIGHER PLANTS. , 1996, Annual review of plant physiology and plant molecular biology.

[22]  K. Takeda,et al.  Diallel Analysis of Salt Tolerance at Germination and the Seedling Stage in Barley (Hordeum vulgare L.) , 1997 .

[23]  W. Thomas,et al.  Comparison of salt tolerance of GPert and non-GPert barleys , 1997 .

[24]  D. Mather,et al.  Regions of the Genome That Affect Grain and Malt Quality in a North American Two-Row Barley Cross , 1997 .

[25]  T. Flowers,et al.  Sodium and potassium transport to the xylem are inherited independently in rice, and the mechanism of sodium: potassium selectivity differs between rice and wheat , 1997 .

[26]  E. Nevo,et al.  AFLP variation in wild barley (Hordeum spontaneum C. Koch) with reference to salt tolerance and associated ecogeography. , 1997, Genome.

[27]  D. C. Gordon,et al.  Mapping physiological traits in barley , 1997 .

[28]  M. Kearsey,et al.  QTL analysis in plants; where are we now? , 1998, Heredity.

[29]  C. Scrimgeour,et al.  A theory for 15N/14N fractionation in nitrate-grown vascular plants , 1998, Planta.

[30]  A. Yeo,et al.  Molecular biology of salt tolerance in the context of whole-plant physiology , 1998 .

[31]  A. Graner,et al.  Molecular mapping of two dwarfing genes differing in their GA response on chromosome 2H of barley , 1999, Theoretical and Applied Genetics.

[32]  P. Christou,et al.  ‘Green revolution’ genes encode mutant gibberellin response modulators , 1999, Nature.

[33]  A. Graner,et al.  Comparative mapping of a gibberellic acid-insensitive dwarfing gene (Dwf2) on chromosome 4HS in barley , 1999, Theoretical and Applied Genetics.

[34]  Chandler,et al.  Gibberellin dose-response curves and the characterization of dwarf mutants of barley , 1999, Plant physiology.

[35]  J. Zhu,et al.  Genetic analysis of plant salt tolerance using Arabidopsis. , 2000, Plant physiology.

[36]  M. Morgante,et al.  A simple sequence repeat-based linkage map of barley. , 2000, Genetics.

[37]  W. Thomas.  6 – Molecular biological tools in cereal breeding , 2000 .

[38]  D. C. Gordon,et al.  Using stable isotope natural abundances (delta 15N and delta 13C) to integrate the stress responses of wild barley (Hordeum spontaneum C. Koch.) genotypes. , 2000, Journal of experimental botany.

[39]  J. Léon,et al.  Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars , 2000, Theoretical and Applied Genetics.

[40]  R. Munns,et al.  Water relations and leaf expansion: importance of time scale. , 2000, Journal of experimental botany.

[41]  L. Gentzbittel,et al.  QTL analysis of photosynthesis and water status traits in sunflower (Helianthus annuus L.) under greenhouse conditions. , 2001, Journal of experimental botany.

[42]  Roeland E. Voorrips,et al.  Software for the calculation of genetic linkage maps , 2001 .

[43]  D. Kudrna,et al.  Molecular mapping of the Oregon Wolfe Barleys: a phenotypically polymorphic doubled-haploid population , 2001, Theoretical and Applied Genetics.

[44]  A. Gómez-Cadenas,et al.  The salt stress-inducible protein kinase gene, Esi47, from the salt-tolerant wheatgrass Lophopyrum elongatum is involved in plant hormone signaling. , 2001, Plant physiology.

[45]  Shizhong Xu.  QTL analysis in plants. , 2002, Methods in molecular biology.

[46]  W. Powell,et al.  Using molecular markers to determine barleys most suitable for malt whisky distilling , 2004, Molecular Breeding.

[47]  W. Powell,et al.  Identification of a QTL decreasing yield in barley linked to Mlo powdery mildew resistance , 1998, Molecular Breeding.

[48]  Seung Youn Han,et al.  Expression of the betaine aldehyde dehydrogenase gene in barley in response to osmotic stress and abscisic acid , 2004, Plant Molecular Biology.

[49]  W. Powell,et al.  A retrospective analysis of spring barley germplasm development from `foundation genotypes' to currently successful cultivars , 2000, Molecular Breeding.

[50]  W. Powell,et al.  The use of AFLPs to examine genetic relatedness in barley , 1997, Molecular Breeding.

[51]  L. Handley,et al.  Comparison of shoot dry weight, Na+ content and δ13C values of ari-e and other semi-dwarf barley mutants under salt-stress , 1997, Euphytica.

[52]  J. Waines,et al.  Genetic analysis of carbon isotope discrimination and agronomic characters in a bread wheat cross , 1994, Theoretical and Applied Genetics.

[53]  J. Dvorak,et al.  Mapping of the K+/Na+ discrimination locus Kna1 in wheat , 1996, Theoretical and Applied Genetics.

[54]  K. Takeda,et al.  Mapping quantitative trait loci for salt tolerance at germination and the seedling stage in barley (Hordeum vulgare L.) , 1997, Euphytica.