The Effects of Biochar and Its Combination with Compost on Lettuce (Lactuca sativa L.) Growth, Soil Properties, and Soil Microbial Activity and Abundance

Impacts of biochar application in combination with organic fertilizer, such as compost, are not fully understood. In this study, we tested the effects of biochar amendment, compost addition, and their combination on lettuce plants grown in a soil poor in nutrients; soil microbiological, chemical, and physical characteristics were analyzed, together with plant growth and physiology. An initial screening was also done to evaluate the effect of biochar and compost toxicity, using cress plants and earthworms. Results showed that compost amendment had clear and positive effects on plant growth and yield and on soil chemical characteristics. However, we demonstrated that also the biochar alone stimulated lettuce leaves number and total biomass, improving soil total nitrogen and phosphorus contents, as well as total carbon, and enhancing related microbial communities. Nevertheless, combining biochar and compost, no positive synergic and summative effects were observed. Our results thus demonstrate that in a soil poor in nutrients the biochar alone could be effectively used to enhance soil fertility and plant growth and biomass yield. However, we can speculate that the combination of compost and biochar may enhance and sustain soil biophysical and chemical characteristics and improve crop productivity over time.

[1]  R. Joergensen,et al.  Compost and phosphorus amendments for stimulating microorganisms and growth of ryegrass in a Ferralsol and a Luvisol , 2012 .

[2]  Gerd Gleixner,et al.  Chars produced by slow pyrolysis and hydrothermal carbonization vary in carbon sequestration potential and greenhouse gases emissions , 2013 .

[3]  A. Mehlich.  Use of Triethanolamine Acetate‐Barium Hydroxide Buffer for the Determination of Some Base Exchange Properties and Lime Requirement of Soil , 1939 .

[4]  P. Brookes,et al.  Investigating the mechanisms for the opposing pH relationships of fungal and bacterial growth in soil , 2010 .

[5]  B. Glaser,et al.  Effects of biochar compared to organic and inorganic fertilizers on soil quality and plant growth in a greenhouse experiment , 2012 .

[6]  Christopher Ennis,et al.  Biochar: Carbon Sequestration, Land Remediation, and Impacts on Soil Microbiology , 2012 .

[7]  Martin-Luther-Universität Halle-Wittenberg.  Synergisms between Compost and Biochar for Sustainable Soil Amelioration , 2012 .

[8]  P. Brookes,et al.  Relationships between soil pH and microbial properties in a UK arable soil , 2008 .

[9]  J. J. Bisogni,et al.  Ammonium , Nitrate , and Phosphate Sorption to and Solute Leaching from Biochars Prepared from Corn Stover ( Zea mays L . ) and Oak Wood ( Quercus spp , 2012 .

[10]  W. Harpole,et al.  Biochar and its effects on plant productivity and nutrient cycling: a meta‐analysis , 2013 .

[11]  M. Reis,et al.  Comparative response of lettuce ( Lactuca sativa ) to inorganic and organic compost fertilization , 2013 .

[12]  R. Bowman.  A Rapid Method to Determine Total Phosphorus in Soils , 1988 .

[13]  P. Pullammanappallil,et al.  Biochar derived from anaerobically digested sugar beet tailings: characterization and phosphate removal potential. , 2011, Bioresource technology.

[14]  L. Giovannetti,et al.  Response of microbial communities to different doses of chromate in soil microcosms , 2006 .

[15]  L. Abbott,et al.  Direct and residual effect of biochar application on mycorrhizal root colonisation, growth and nutrition of wheat , 2010 .

[16]  H. Andry,et al.  Effect of cow manure biochar on maize productivity under sandy soil condition , 2011 .

[17]  Fulvia Tambone,et al.  Effect of compost application rate on carbon degradation and retention in soils. , 2009, Waste management.

[18]  V. Bailey,et al.  Reconciling apparent variability in effects of biochar amendment on soil enzyme activities by assay optimization , 2011 .

[19]  Davey L. Jones,et al.  Life in the 'charosphere' - Does biochar in agricultural soil provide a significant habitat for microorganisms? , 2013 .

[20]  P. Brookes,et al.  Contrasting Soil pH Effects on Fungal and Bacterial Growth Suggest Functional Redundancy in Carbon Mineralization , 2009, Applied and Environmental Microbiology.

[21]  A. Miltner,et al.  Effects of wood char and activated carbon on the hydrolysis of cellobiose by β-glucosidase from Aspergillus niger. , 2011 .

[22]  G. E. Rayment,et al.  Australian laboratory handbook of soil and water chemical methods. , 1992 .

[23]  J. Ippolito,et al.  Biochar and manure affect calcareous soil and corn silage nutrient concentrations and uptake. , 2012, Journal of environmental quality.

[24]  M. Velde,et al.  A quantitative review of the effects of biochar application to soils on crop productivity using meta-analysis , 2011 .

[25]  F. Miglietta,et al.  More plant growth but less plant defence? First global gene expression data for plants grown in soil amended with biochar , 2015 .

[26]  Ding Wenchuan,et al.  Characteristics and performances of biofilm carrier prepared from agro-based biochar. , 2011 .

[27]  R. Lal,et al.  Biochar Impacts on Soil Physical Properties and Greenhouse Gas Emissions , 2013 .

[28]  Gareth Edwards-Jones,et al.  Biochar-mediated changes in soil quality and plant growth in a three year field trial , 2012 .

[29]  Saran Sohi,et al.  The Impact of Biochar Application on Soil Properties and Plant Growth of Pot Grown Lettuce (Lactuca sativa) and Cabbage (Brassica chinensis) , 2013 .

[30]  Francesco Montemurro,et al.  Long-term effects of organic amendments on soil fertility. A review , 2010, Agronomy for Sustainable Development.

[31]  J. Lehmann,et al.  Ameliorating physical and chemical properties of highly weathered soils in the tropics with charcoal – a review , 2002, Biology and Fertility of Soils.

[32]  C. Masiello,et al.  Temperature sensitivity of black carbon decomposition and oxidation. , 2010, Environmental science & technology.

[33]  John Gaunt,et al.  Bio-char Sequestration in Terrestrial Ecosystems – A Review , 2006 .

[34]  J. J. Bisogni,et al.  Ammonium, Nitrate, and Phosphate Sorption to and Solute Leaching from Biochars Prepared from Corn Stover ( L.) and Oak Wood ( spp.). , 2013, Journal of environmental quality.

[35]  Hongyan Jin.  Characterization Of Microbial Life Colonizing Biochar And Biochar-Amended Soils , 2010 .

[36]  Yong-guan Zhu,et al.  Biochar impacts soil microbial community composition and nitrogen cycling in an acidic soil planted with rape. , 2014, Environmental science & technology.

[37]  Irina Subbotina,et al.  Black carbon decomposition and incorporation into soil microbial biomass estimated by 14C labeling , 2009 .

[38]  S. R. Olsen,et al.  Estimation of available phosphorus in soils by extraction with sodium bicarbonate , 1954 .

[39]  L. Beesley,et al.  Carbon and trace element fluxes in the pore water of an urban soil following greenwaste compost, woody and biochar amendments, inoculated with the earthworm Lumbricus terrestris , 2011 .

[40]  F. Miglietta,et al.  Biochar stimulates plant growth but not fruit yield of processing tomato in a fertile soil , 2015 .

[41]  Caroline A. Masiello,et al.  Biochar effects on soil biota – A review , 2011 .

[42]  G. Bonanomi,et al.  Organic amendments as sustainable tool to recovery fertility in intensive agricultural systems , 2015 .

[43]  Akwasi A Boateng,et al.  Biochar: a synthesis of its agronomic impact beyond carbon sequestration. , 2012, Journal of environmental quality.

[44]  Changrong Yan,et al.  Long-term effect of chemical fertilizer, straw, and manure on soil chemical and biological properties in northwest China , 2010 .

[45]  S. Carpenter,et al.  Global Consequences of Land Use , 2005, Science.

[46]  T. Clough,et al.  Biochar and the nitrogen cycle: introduction. , 2010, Journal of environmental quality.

[47]  J. A. Alburquerque,et al.  Composting of animal manures and chemical criteria for compost maturity assessment. A review. , 2009, Bioresource technology.

[48]  F. Miglietta,et al.  Short-term effects of biochar on grapevine fine root dynamics and arbuscular mycorrhizae production , 2017 .

[49]  S. Sohi,et al.  Biochar – synergies and trade‐offs between soil enhancing properties and C sequestration potential , 2015 .

[50]  F. Nigro,et al.  Suppressive biomasses and antagonist bacteria for an eco-compatible control of Verticillium dahliae on nursery-grown olive plants , 2013, International Journal of Environmental Science and Technology.

[51]  S. Sohi.  BIOCHAR, CLIMATE CHANGE AND SOIL: A REVIEW TO GUIDE FUTURE RESEARCH , 2009 .

[52]  Davey L. Jones,et al.  Biochar effects on soil nutrient transformations , 2009 .

[53]  S. M. Tiquia.  Evolution of extracellular enzyme activities during manure composting , 2002, Journal of applied microbiology.

[54]  C. Stewart,et al.  Co‐generated fast pyrolysis biochar mitigates green‐house gas emissions and increases carbon sequestration in temperate soils , 2013 .

[55]  C. Kammann,et al.  Biochar and biochar-compost as soil amendments to a vineyard soil: Influences on plant growth, nutrient uptake, plant health and grape quality , 2014 .

[56]  M. Fiers,et al.  Biochar induced soil microbial community change: Implications for biogeochemical cycling of carbon, nitrogen and phosphorus , 2011 .

[57]  P. Clode,et al.  Microscopy Observations of Habitable Space in Biochar for Colonization by Fungal Hyphae From Soil , 2014 .

[58]  J. Lehmann,et al.  Biochar Effects on Nutrient Leaching , 2012 .

[59]  M. Rillig,et al.  Characteristics of Biochar: Biological Properties , 2012 .

[60]  C. García,et al.  Soil microbial activity after restoration of a semiarid soil by organic amendments , 2003 .

[61]  Jago Jonathan Birk,et al.  State of the scientific knowledge on properties and genesis of Anthropogenic Dark Earths in Central Amazonia (terra preta de Índio) , 2012 .

[62]  K. Shepherd,et al.  Integrated Soil Fertility Management , 2010 .

[63]  G. Lima,et al.  Characterization of composted olive mill wastes to predict potential plant disease suppressiveness , 2011 .

[64]  D. Porath,et al.  Chlorophyll determination in intact tissues using n,n-dimethylformamide. , 1980, Plant physiology.

[65]  W. Inskeep,et al.  Extinction coefficients of chlorophyll a and B in n,n-dimethylformamide and 80% acetone. , 1985, Plant physiology.

[66]  S. Sohi,et al.  A review of biochar and its use and function in soil , 2010 .

[67]  W. Wanek,et al.  Biochar Decelerates Soil Organic Nitrogen Cycling but Stimulates Soil Nitrification in a Temperate Arable Field Trial , 2014, PloS one.

[68]  P. N. Nelson,et al.  The ameliorating effects of biochar and compost on soil quality and plant growth on a Ferralsol , 2015 .

[69]  Bernd Huwe,et al.  Short‐term effect of biochar and compost on soil fertility and water status of a Dystric Cambisol in NE Germany under field conditions , 2012 .

[70]  C. F. Liao.  Devarda's Alloy Method for Total Nitrogen Determination1 , 1981 .