A local redox cycling-based electrochemical chip device with nanocavities for multi-electrochemical evaluation of embryoid bodies.

An electrochemical device, which consists of electrode arrays, nanocavities, and microwells, was developed for multi-electrochemical detection with high sensitivity. A local redox cycling-based electrochemical (LRC-EC) system was used for multi-electrochemical detection and signal amplification. The LRC-EC system consists of n(2) sensors with only 2n bonding pads for external connection. The nanocavities fabricated in the sensor microwells enable significant improvement of the signal amplification compared with the previous devices we have developed. The present device was successfully applied for evaluation of embryoid bodies (EBs) from embryonic stem (ES) cells via electrochemical measurements of alkaline phosphatase (ALP) activity in the EBs. In addition, the EBs were successfully trapped in the sensor microwells of the device using dielectrophoresis (DEP) manipulation, which led to high-throughput cell analysis. This device is considered to be useful for multi-electrochemical detection and imaging for bioassays including cell analysis.

[1]  Tomoyuki Yasukawa,et al.  Detection of hormone active chemicals using genetically engineered yeast cells and microfluidic devices with interdigitated array electrodes , 2009, Electrophoresis.

[2]  N. Kasai,et al.  Hydrogen peroxide distribution and neuronal cell death in a rat hippocampal slice , 2005 .

[3]  Shinya Yoshida,et al.  Densified electrochemical sensors based on local redox cycling between vertically separated electrodes in substrate generation/chip collection and extended feedback modes. , 2014, Analytical chemistry.

[4]  H. A. Pohl.  The Motion and Precipitation of Suspensoids in Divergent Electric Fields , 1951 .

[5]  Edgar D Goluch,et al.  Stochastic sensing of single molecules in a nanofluidic electrochemical device. , 2011, Nano letters.

[6]  Young-Ho Cho,et al.  A continuous cell separation chip using hydrodynamic dielectrophoresis (DEP) process , 2005 .

[7]  K. Torimitsu,et al.  Real-time electrochemical imaging using an individually addressable multi-channel electrode. , 2000, Biosensors & bioelectronics.

[8]  D. Wilkin,et al.  Neuron , 2001, Brain Research.

[9]  S. Lemay,et al.  Response time of nanofluidic electrochemical sensors. , 2012, Lab on a chip.

[10]  Rashid Bashir,et al.  Patterned Three‐Dimensional Encapsulation of Embryonic Stem Cells using Dielectrophoresis and Stereolithography , 2013, Advanced healthcare materials.

[11]  Tomoyuki Yasukawa,et al.  Electrochemical single-cell gene-expression assay combining dielectrophoretic manipulation with secreted alkaline phosphatase reporter system. , 2009, Biosensors & bioelectronics.

[12]  Boris Hofmann,et al.  Nanocavity redox cycling sensors for the detection of dopamine fluctuations in microfluidic gradients. , 2010, Analytical chemistry.

[13]  W. Heineman,et al.  Small-volume voltammetric detection of 4-aminophenol with interdigitated array electrodes and its application to electrochemical enzyme immunoassay. , 1993, Analytical chemistry.

[14]  Robert L Sah,et al.  Probing the role of multicellular organization in three-dimensional microenvironments , 2006, Nature Methods.

[15]  Droplet array on local redox cycling-based electrochemical (LRC-EC) chip device. , 2014, Lab on a chip.

[16]  K. Ino,et al.  Electrochemical detection for dynamic analyses of a redox component in droplets using a local redox cycling-based electrochemical (LRC-EC) chip device. , 2012, Chemical communications.

[17]  Joseph Wang,et al.  Electrochemical detection for microscale analytical systems: a review. , 2002, Talanta.

[18]  W. Dauer,et al.  Parkinson's Disease Mechanisms and Models , 2003, Neuron.

[19]  Dileep Mampallil,et al.  Electrochemical single-molecule detection in aqueous solution using self-aligned nanogap transducers. , 2013, ACS nano.

[20]  Mark R. Anderson,et al.  Electrochemical Glucose Sensors—Developments Using Electrostatic Assembly and Carbon Nanotubes for Biosensor Construction , 2010, Sensors.

[21]  H. S. Wolff,et al.  iRun: Horizontal and Vertical Shape of a Region-Based Graph Compression , 2022, Sensors.

[22]  Pradyumna S. Singh,et al.  Stochastic amperometric fluctuations as a probe for dynamic adsorption in nanofluidic electrochemical systems. , 2011, Journal of the American Chemical Society.

[23]  Hitoshi Shiku,et al.  Local redox-cycling-based electrochemical chip device with deep microwells for evaluation of embryoid bodies. , 2012, Angewandte Chemie.

[24]  Rashid Bashir,et al.  A multifunctional micro-fluidic system for dielectrophoretic concentration coupled with immuno-capture of low numbers of Listeria monocytogenes. , 2006, Lab on a chip.

[25]  Dileep Mampallil,et al.  Redox couples with unequal diffusion coefficients: effect on redox cycling. , 2013, Analytical chemistry.

[26]  H. Kurosawa,et al.  A simple method for forming embryoid body from mouse embryonic stem cells. , 2003, Journal of bioscience and bioengineering.

[27]  Hisao Tabei,et al.  Electrochemical behavior of reversible redox species at interdigitated array electrodes with different geometries: consideration of redox cycling and collection efficiency , 1990 .

[28]  Andreas Offenhäusser,et al.  Nanocavity crossbar arrays for parallel electrochemical sensing on a chip , 2014, Beilstein journal of nanotechnology.

[29]  Pradyumna S. Singh,et al.  Electrochemical correlation spectroscopy in nanofluidic cavities. , 2009, Analytical chemistry.

[30]  Andreas Offenhäusser,et al.  Redox cycling in nanoporous electrochemical devices. , 2014, Nanoscale.

[31]  Robert L Sah,et al.  Geometric and material determinants of patterning efficiency by dielectrophoresis. , 2004, Biophysical journal.

[32]  Aaas News,et al.  Book Reviews , 1893, Buffalo Medical and Surgical Journal.

[33]  Cheng-Hsien Liu,et al.  Rapid heterogeneous liver-cell on-chip patterning via the enhanced field-induced dielectrophoresis trap. , 2006, Lab on a chip.

[34]  F. Mizutani,et al.  Simple detection of surface antigens on living cells by applying distinct cell positioning with negative dielectrophoresis. , 2012, Analytical chemistry.

[35]  K. Ino,et al.  A Pt layer/Pt disk electrode configuration to evaluate respiration and alkaline phosphatase activities of mouse embryoid bodies. , 2012, Talanta.

[36]  Hitoshi Shiku,et al.  Accumulation and detection of secreted proteins from single cells for reporter gene assays using a local redox cycling-based electrochemical (LRC-EC) chip device. , 2012, Lab on a chip.

[37]  Frédéric Kanoufi,et al.  Cyclic voltammetry and scanning electrochemical microscopy of ferrocenemethanol at monolayer and bilayer-modified gold electrodes , 2003 .

[38]  T. Matsue,et al.  Paired cell aligament using a jagged microarray electrode , 1994 .

[39]  Joel Voldman,et al.  nDEP microwells for single-cell patterning in physiological media. , 2007, Lab on a chip.

[40]  J. Korlach,et al.  DNA fragment sizing by single molecule detection in submicrometer-sized closed fluidic channels. , 2002, Analytical chemistry.

[41]  O. Niwa,et al.  Fabrication and characteristics of vertically separated interdigitated array electrodes , 1989 .

[42]  Kevin Barraclough,et al.  I and i , 2001, BMJ : British Medical Journal.

[43]  Diego Krapf,et al.  Mesoscopic concentration fluctuations in a fluidic nanocavity detected by redox cycling. , 2007, Nano letters.

[44]  Ali Khademhosseini,et al.  Dielectrophoretically Aligned Carbon Nanotubes to Control Electrical and Mechanical Properties of Hydrogels to Fabricate Contractile Muscle Myofibers , 2013, Advanced materials.

[45]  Tomoyuki Yasukawa,et al.  Electrophoretic cell manipulation and electrochemical gene-function analysis based on a yeast two-hybrid system in a microfluidic device. , 2008, Analytical chemistry.

[46]  Shuo Kang,et al.  Integrated biodetection in a nanofluidic device. , 2014, ACS nano.

[47]  Pradyumna S. Singh,et al.  Noise characteristics of nanoscaled redox-cycling sensors: investigations based on random walks. , 2013, Journal of the American Chemical Society.

[48]  O. Français,et al.  Integrated microdevice for long-term automated perfusion culture without shear stress and real-time electrochemical monitoring of cells. , 2011, Analytical chemistry.

[49]  Pradyumna S. Singh,et al.  Lithography-based nanoelectrochemistry. , 2011, Analytical chemistry.

[51]  Pradyumna S. Singh,et al.  Stochasticity in single-molecule nanoelectrochemistry: origins, consequences, and solutions. , 2012, ACS nano.

[52]  Hitoshi Shiku,et al.  Addressable electrode array device with IDA electrodes for high-throughput detection. , 2011, Lab on a chip.

[53]  Koichi Aoki,et al.  Quantitative analysis of reversible diffusion-controlled currents of redox soluble species at interdigitated array electrodes under steady-state conditions , 1988 .

[54]  W. Marsden.  I and J , 2012 .

[55]  Bernhard Wolfrum,et al.  Nanofluidic redox cycling amplification for the selective detection of catechol. , 2008, Analytical chemistry.