A compact surface plasmon resonance and surface-enhanced Raman scattering sensing device

This paper presents an optical device capable of the simultaneous measurement of the surface plasmon resonance (SPR) spectrum, which provides information regarding the change in the dielectric constant of the binding analytes, and the surface-enhanced Raman scattering (SERS) spectrum, which yields analytical data regarding the structural changes of the analytes. SPR sensing is an established technology in the field of direct real-time analysis of biomolecular interactions such as antibodies/antigens, DNA hybridization, receptors/ligands, etc. Meanwhile, SERS sensing techniques represent a powerful means of acquiring and diagnosing structural information relating to analyte binding. This study adopts the attenuated total reflection (ATR) method and an Au nanocluster-embedded dielectric sensing film in developing a biosensor which integrates the SPR and SERS sensing techniques. The results confirm the effectiveness of the proposed multi-functional device in developing a detailed understanding of the mechanisms of biomolecular recognition.

[1]  A J Paine,et al.  Mechanisms of chromium toxicity, carcinogenicity and allergenicity: Review of the literature from 1985 to 2000 , 2001, Human & experimental toxicology.

[2]  J. M. McDonnell,et al.  Surface plasmon resonance: towards an understanding of the mechanisms of biological molecular recognition. , 2001, Current opinion in chemical biology.

[3]  S. Dong,et al.  Direct electrochemistry and surface plasmon resonance characterization of alternate layer-by-layer self-assembled DNA-myoglobin thin films on chemically modified gold surfaces , 2003 .

[4]  Novotny,et al.  Local Excitation, Scattering, and Interference of Surface Plasmons. , 1996, Physical review letters.

[5]  K. Schierbaum,et al.  Sensing molecular properties by ATR-SPP Raman spectroscopy on electrochemically structured sensor chips , 2003 .

[6]  T. Addona,et al.  The integration of SPR biosensors with mass spectrometry: possible applications for proteome analysis. , 2000, Trends in biotechnology.

[7]  S. Nagao,et al.  Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) Spectroscopy of Functional Groups of Humic Acid Dissolving in Aqueous Solution , 2002 .

[8]  R. Dasari,et al.  Single Molecule Detection Using Surface-Enhanced Raman Scattering (SERS) , 1997 .

[9]  H. Sota,et al.  Detection of conformational changes in an immobilized protein using surface plasmon resonance. , 1998, Analytical chemistry.

[10]  Günter Gauglitz,et al.  Surface plasmon resonance sensors: review , 1999 .

[11]  J. B. Pitner,et al.  Using receptor conformational change to detect low molecular weight analytes by surface plasmon resonance. , 2001, Analytical chemistry.

[12]  K-A Lai,et al.  A novel ultrahigh-resolution surface plasmon resonance biosensor with an Au nanocluster-embedded dielectric film. , 2004, Biosensors & bioelectronics.

[13]  O. Niwa,et al.  Detection of electrochemical enzymatic reactions by surface plasmon resonance measurement. , 2001, Analytical chemistry.