Cell surface properties of high‐ and low‐metastatic cell lines selected from a spontaneous mouse lung carcinoma

The surface oligosaccharide residues, glycoproteins and sialyl components of CMT64 lung carcinoma cells and high‐metastatic sublines CMT167 and CMT181 have been studied in culture. (I) The total cellular sialic acid content did not differ appreciably between the three lines. However, the accessibility of surface sialyl groups, measured by metabolic incorporation of [3H]NAcmannosamine followed by neuraminidase hydrolysis, was decreased from 42% in CMT64 to 25% hydrolyzed in CMT181. (2) The major plasma membrane glycoproteins of the lines were radiolabelled by lactoperoxidase iodination, metabolic incorporation of [3H]fucose or labelling in the terminal sialyl residues by the NalO4‐NaB[3H]4 method and the labelled glycoproteins were analyzed by two‐dimensional gel electrophoresis. Each labelling technique identified a complex pattern of glycoproteins including a prominently labelled group of high‐molecular‐weight acidic sialoglycoproteins: GP200/4.9–5.1 (apparent molecular weight ×10−3/pI of iodoprotein); GP150/5.1–5.6; GP130/5.0–5.6; GP110/5.0; GP100/4.8 and GP100/5.0–5.4. (3) The neuraminidase‐susceptible glycoproteins on CMT64 and CMT181 were identified in the isoelectric focusing separation of the two‐dimensional gel separation by the charge difference caused by desialylation. The glycoproteins most susceptible to neuraminidase were the high‐molecular‐weight acidic glycoproteins which showed marked charge heterogeneity: GP150/5.1–5.6, GP130/5.0–5.6; GP100/5.0–5.4 and GP100/4.8. (4) Using these procedures we did not detect modifications between CMT181 and CMT64 and we conclude that the cultured cells of the sublines do not display marked surface glycoprotein alterations that reflect their enhanced spontaneous metastatic potential.

[1]  L. M. Franks,et al.  Heterogeneity in a spontaneous mouse lung carcinoma: selection and characterisation of stable metastatic variants. , 1984, British Journal of Cancer.

[2]  L. M. Franks,et al.  Identification of exposed surface glycoproteins of four human bladder carcinoma cell lines. , 1983, Biochimica et biophysica acta.

[3]  G. Nicolson.  Cancer metastasis: Organ colonization and the cell-surface properties of mallignant cells , 1982 .

[4]  R. L. Felsted,et al.  A comparison of K-562 and HL-60 human leukemic cell surface membrane proteins by two-dimensional electrophoresis. , 1982, The Journal of biological chemistry.

[5]  G. Nicolson,et al.  Clonal drift of cell surface, melanogenic, and experimental metastatic properties of in vivo-selected, brain meninges-colonizing murine B16 melanoma. , 1982, Cancer research.

[6]  J. Fishman,et al.  Recycling of surface sialoglycoconjugates in HTC and HeLa cells. , 1982, The Journal of biological chemistry.

[7]  W. Franke.  Cytoskeletal differentiations in epithelial cells , 1982 .

[8]  G. Nicolson,et al.  Phenotypic drift of metastatic and cell‐surface properties of mammary adenocarcinoma cell clones during growth in vitro , 1981, International journal of cancer.

[9]  G. Nicolson,et al.  Distribution of fibronectin on clonal cell lines of a rat mammary adenocarcinoma growing in vitro and in vivo at primary and metastatic sites. , 1981, Cancer research.

[10]  R. Moll,et al.  Antibodies to high molecular weight polypeptides of desmosomes: specific localization of a class of junctional proteins in cells and tissue. , 1981, Differentiation; research in biological diversity.

[11]  R. Roberts,et al.  The purification and immunocytochemical localization of the major iodinatable cell surface glycoproteins of Chinese hamster ovary cells. , 1981, Journal of cell science.

[12]  M. Rieber.  Metastatic potential correlates with cell-surface protein alterations in B16 melanoma variants , 1981, Nature.

[13]  S. Kennel,et al.  Analysis of surface proteins of mouse lung carcinomas using monoclonal antibodies. , 1981, Cancer research.

[14]  T. Irimura,et al.  Effects of tunicamycin on B16 metastatic melanoma cell surface glycoproteins and blood-borne arrest and survival properties. , 1981, Cancer research.

[15]  I. Fidler,et al.  The implications of tumor heterogeneity for studies on the biology and therapy of cancer metastasis , 1981 .

[16]  G. Yogeeswaran,et al.  Metastatic potential is positively correlated with cell surface sialylation of cultured murine tumor cell lines. , 1981, Science.

[17]  Z. Pavelic,et al.  A correlation between cell surface sialyltransferase, sialic acid, and glycosidase activities and the implantability of B16 murine melanoma. , 1981, Cancer research.

[18]  P. Stanley,et al.  Microheterogeneity among carbohydrate structures at the cell surface may be important in recognition phenomena , 1981, Cell.

[19]  J. Roboz,et al.  Elimination of 2-deoxyribose interference in the thiobarbituric acid determination of N-acetylneuraminic acid in tumor cells by pH-dependent extraction with cyclohexanone. , 1981, Analytical biochemistry.

[20]  T. Irimura,et al.  The role of glycoconjugates in metastatic melanoma blood-borne arrest and cell surface properties. , 1981, Journal of supramolecular structure and cellular biochemistry.

[21]  H. Hara,et al.  Effect of temperature on normal and SV40-transformed human fibroblasts. , 1981, Biochimica et biophysica acta.

[22]  J. Talmadge,et al.  In vitro characteristics of metastatic variant subclones of restricted genetic origin. , 1981, Journal of supramolecular structure and cellular biochemistry.

[23]  G. Nicolson,et al.  Malignancies of metastatic murine lymphosarcoma cell lines and clones correlate with decreased cell surface display of RNA tumor virus envelope glycoprotein gp70. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[24]  G. Yogeeswaran,et al.  Cell surface sialic acid expression of lectin-resistant variant clones of B16 melanoma with altered metastasizing potential. , 1980, Biochemical and biophysical research communications.

[25]  J. Glenney,et al.  Identification and partial characterization of the major galactoproteins present at the surface of AS-30D hepatocellular carcinoma cells. , 1980, Cancer research.

[26]  R. Chesnut,et al.  Changes in expression of a major sialoglycoprotein associated with ascites forms of a mammary adenocarcinoma. , 1980, Biochimica et biophysica acta.

[27]  M. Olympio,et al.  Isolation of a domain of the plasma membrane in Chinese hamster ovary cells which contains iodinatable, acidic glycoproteins of high molecular weight. , 1980, Biochimica et biophysica acta.

[28]  J. Glenney,et al.  Accessibility of plasma membrane sialoglycoconjugates of Novikoff tumor cells to exogenous neuraminidase and proteases. , 1980, Biochimica et biophysica acta.

[29]  G. Nicolson,et al.  Selection and in vivo properties of lectin-attachment variants of malignant murine lymphosarcoma cell lines. , 1980, Journal of the National Cancer Institute.

[30]  C. Bucana,et al.  Cell surface properties of B16 melanoma variants with differing metastatic potential. , 1980, Cancer research.

[31]  G Poste,et al.  Arrest and metastasis of blood-borne tumor cells are modified by fusion of plasma membrane vesicles from highly metastatic cells. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[32]  J I Garrels,et al.  Two dimensional gel electrophoresis and computer analysis of proteins synthesized by clonal cell lines. , 1979, The Journal of biological chemistry.

[33]  G. Yogeeswaran,et al.  Cell surface sialylation of glycoproteins and glycosphingolipids in cultured metastatic variant rna‐virus transformed non‐producer BALB/c 3T3 cell lines , 1979, International journal of cancer.

[34]  P. W. Kent,et al.  Neuraminidase inhibition by chemically sulphated glycopeptides. , 1979, The Biochemical journal.

[35]  S. Kennel.  Characterization of a tumor cell surface protein with heterologous antisera to a spontaneous BALB/c lung carcinoma. , 1979, Cancer research.

[36]  M. Monsigny,et al.  Properties of succinylated wheat-germ agglutinin. , 1979, European journal of biochemistry.

[37]  W. J. Grimes,et al.  Two-dimensional electrophoresis of membrane proteins from normal and transformed cells. , 1979, Cancer research.

[38]  H. Baumann,et al.  Localization of membrane glycoproteins by in situ neuraminidase treatment of rat hepatoma tissue culture cells and two-dimensional gel electrophoretic analysis of the modified proteins. , 1979, The Journal of biological chemistry.

[39]  W. Loewenstein,et al.  Junctional intercellular communication and the control of growth. , 1979, Biochimica et biophysica acta.

[40]  G. Nicolson,et al.  Selection of malignant melanoma variant cell lines for ovary colonization. , 1979, Journal of supramolecular structure.

[41]  G. Tuszynski,et al.  Glycopeptide changes and malignant transformation. A possible role for carbohydrate in malignant behavior. , 1978, Biochimica et biophysica acta.

[42]  N. Tolbert,et al.  A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. , 1978, Analytical biochemistry.

[43]  B. Stein,et al.  Altered cell surface organization of gangliosides and sialylglycoproteins of mouse metastatic melanoma variant lines selected in vivo for enhanced lung implantation. , 1978, Cancer research.

[44]  G. Nicolson,et al.  Selection and altered properties of brain-colonising metastatic melanoma , 1978, Nature.

[45]  D M Gersten,et al.  The biology of cancer invasion and metastasis. , 1978, Advances in cancer research.

[46]  T. Chen,et al.  In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. , 1977, Experimental cell research.

[47]  R. Hynes.  Cell surface proteins and malignant transformation. , 1976, Biochimica et biophysica acta.

[48]  G. Nicolson.  Trans-membrane control of the receptors on normal and tumor cells. II. Surface changes associated with transformation and malignancy. , 1976, Biochimica et biophysica acta.

[49]  P. Riddle,et al.  Metastasizing tumors from serum-supplemented and serum-free cell lines from a C57BL mouse lung tumor. , 1976, Cancer research.

[50]  P. O’Farrell.  High resolution two-dimensional electrophoresis of proteins. , 1975, The Journal of biological chemistry.

[51]  J. Luft.  Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action , 1971, The Anatomical record.