To evaluate the relationship between the enzymatic Vitros assay for creatinine determination and other methods, we determined creatinine concentration in 400 heparin samples. Plasma creatinine level was successively determined on the Vitros 750 analyzer (Johnson & Johnson Co., Rochester, NY) and on the Olympus AU2700 analyzer (Olympus France, Rungis, France), using three reagents in the same assay: Olympus‐Jaffé and two enzymatic commercial kits—Crea Plus (Roche Diagnostics, Meylan, France) and Enzymatic Creatinine (Randox, Mauguio, France). Comparison of Jaffé and enzymatic measurements of plasma creatinine levels revealed a high correlation when considering all values ranged from 20–1,000 μmol/l (r > 0.99). However, for values <60 μmol/l, enzymatic reagents provided best results. Enzymatic methodology is a better clinical choice for the accurate measurement of creatinine, especially for neonates, pediatrics, and hematology units. Because analytical performance and the costs of Randox creatinine were satisfactory for our laboratory, this method, adapted on the Olympus analyzer, was chosen for routine determination of creatinine levels. According to the Valtec protocol (8), no interferences such as hemolysis, lipemia, or bilirubin were detected for Enzymatic Creatinine Randox. J. Clin. Lab. Anal. 17:235–240, 2003. © 2003 Wiley‐Liss, Inc.
[1]
S. Guérin,et al.
Technique sélectionnée pour le dosage de la créatinine dans le plasma ou le sérum. Choix des conditions optimales de mesure
,
1996
.
[2]
N. Madias,et al.
Serum creatinine as an index of renal function: new insights into old concepts.
,
1992,
Clinical chemistry.
[3]
A. V. van Zanten,et al.
Interferences in current methods for measurements of creatinine.
,
1991,
Clinical chemistry.
[4]
W. Guder,et al.
Multicentre evaluation of an enzymatic method for creatinine determination using a sensitive colour reagent.
,
1986,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie.
[5]
K. Spencer.
Analytical Reviews in Clinical Biochemistry: The Estimation of Creatinine
,
1986,
Annals of clinical biochemistry.