Collagen membranes crosslinked by β-cyclodextrin polyrotaxane monoaldehyde with good biocompatibilities and repair capabilities for cornea repair

Collagen is an excellent candidate for a cornea repair material. However, current use of collagen materials for cornea repair is limited by their insufficient mechanical properties. Herein, we used the β-cyclodextrin polyrotaxane monoaldehyde (β-CD-PR-4) a compound that has good biocompatibility for crosslinking collagen in order to obtain the appropriate cornea repair material, Col-β-CD-PR-4. We characterized the physical and chemical properties of Col-β-CD-PR-4 and compared it with that of collagen crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and glutaraldehyde (GA). The results showed that Col-β-CD-PR-4 had an appropriate water content and good light transmittance. Compared to the other crosslinkers, Col-β-CD-PR-4 had better mechanical properties, especially tensile strength, suturability, and enzyme tolerance capacity. Col-β-CD-PR-4 also had high suture retention strength as demonstrated by suture experiments showing tight suturing on rabbit cornea. Moreover, Col-β-CD-PR-4 displayed good biocompatibility to human corneal epithelial cells in vitro. In vivo lamellar keratoplasty (LKP) results showed that cornea had epithelized completely in approximately 16 days, and the transparency was restored quickly in 4 weeks. No inflammation and corneal neovascularization were observed and corneal rejection reaction and keratoconus were not observed. Overall, Col-β-CD-PR-4 showed excellent potential for use in corneal tissue engineering.

[1]  Yingjun Wang,et al.  Collagen based film with well epithelial and stromal regeneration as corneal repair materials: Improving mechanical property by crosslinking with citric acid. , 2015, Materials science & engineering. C, Materials for biological applications.

[2]  Q. Le,et al.  T-style keratoprosthesis based on surface-modified poly (2-hydroxyethyl methacrylate) hydrogel for cornea repairs. , 2015, Materials science & engineering. C, Materials for biological applications.

[3]  C. Chen,et al.  Preparation of PVA hydrogel with high-transparence and investigations of its transparent mechanism , 2015 .

[4]  Yingjun Wang,et al.  Improving the mechanical properties of collagen-based membranes using silk fibroin for corneal tissue engineering. , 2015, Journal of biomedical materials research. Part A.

[5]  Yingjun Wang,et al.  β-Cyclodextrin polyrotaxane monoaldehyde: a novel bio-crosslinker with high biocompatibility , 2014 .

[6]  F. Filippin-Monteiro,et al.  Cytotoxicity of PVPAC-treated bovine pericardium: a potential replacement for glutaraldehyde in biological heart valves. , 2014, Journal of biomedical materials research. Part B, Applied biomaterials.

[7]  Isabelle Brunette,et al.  Stable corneal regeneration four years after implantation of a cell-free recombinant human collagen scaffold. , 2014, Biomaterials.

[8]  Yunfeng Shi,et al.  Preparation and characterization of a hydrogel carrier to deliver gatifloxacin and its application as a therapeutic contact lens for bacterial keratitis therapy , 2013, Biomedical materials.

[9]  J. Forrester,et al.  Crosslinked collagen hydrogels as corneal implants: effects of sterically bulky vs. non-bulky carbodiimides as crosslinkers. , 2013, Acta biomaterialia.

[10]  Sandeep Kumar Vashist,et al.  Comparison of 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Based Strategies to Crosslink Antibodies on Amine-Functionalized Platforms for Immunodiagnostic Applications , 2012, Diagnostics.

[11]  S. Nam,et al.  Preparation of collagen-immobilized poly(ethylene glycol)/poly(2- hydroxyethyl methacrylate) interpenetrating network hydrogels for potential application of artificial cornea , 2012 .

[12]  C. Connon,et al.  Plastic compression of a collagen gel forms a much improved scaffold for ocular surface tissue engineering over conventional collagen gels. , 2010, Journal of biomedical materials research. Part A.

[13]  Shay Soker,et al.  Bioengineering endothelialized neo-corneas using donor-derived corneal endothelial cells and decellularized corneal stroma. , 2010, Biomaterials.

[14]  Rejean Munger,et al.  A Biosynthetic Alternative to Human Donor Tissue for Inducing Corneal Regeneration: 24-Month Follow-Up of a Phase 1 Clinical Study , 2010, Science Translational Medicine.

[15]  Fengfu Li,et al.  Synthetic neoglycopolymer-recombinant human collagen hybrids as biomimetic crosslinking agents in corneal tissue engineering. , 2009, Biomaterials.

[16]  J. Scaiano,et al.  Collagen-phosphorylcholine interpenetrating network hydrogels as corneal substitutes. , 2009, Biomaterials.

[17]  Fengfu Li,et al.  PEG-stabilized carbodiimide crosslinked collagen-chitosan hydrogels for corneal tissue engineering. , 2008, Biomaterials.

[18]  Kevin W. Ross,et al.  Infectious disease risk factors of corneal graft donors. , 2008, Archives of ophthalmology.

[19]  H. Sheardown,et al.  Dendrimer crosslinked collagen as a corneal tissue engineering scaffold: mechanical properties and corneal epithelial cell interactions. , 2006, Biomaterials.

[20]  Rejean Munger,et al.  A simple, cross-linked collagen tissue substitute for corneal implantation. , 2006, Investigative ophthalmology & visual science.

[21]  H. Sheardown,et al.  Progress in the development of a corneal replacement: keratoprostheses and tissue-engineered corneas , 2006, Expert review of medical devices.

[22]  M. Fini,et al.  How the Cornea Heals: Cornea-specific Repair Mechanisms Affecting Surgical Outcomes , 2005, Cornea.

[23]  D. Landolt,et al.  Time-dependent morphology and adhesion of osteoblastic cells on titanium model surfaces featuring scale-resolved topography. , 2004, Biomaterials.

[24]  J. Weng,et al.  Characterization of surface oxide films on titanium and adhesion of osteoblast. , 2003, Biomaterials.

[25]  B. Seitz,et al.  Corneal calcification after amniotic membrane transplantation , 2003, The British journal of ophthalmology.

[26]  A Rajaram,et al.  Influence of different crosslinking treatments on the physical properties of collagen membranes. , 2003, Biomaterials.

[27]  P. Kingshott,et al.  Studies on new polymeric biomaterials with tunable hydrophilicity, and their possible utility in corneal repair surgery. , 2002, Biomaterials.

[28]  W. Grundfest,et al.  Ablation spectra of the human cornea. , 2001, Journal of biomedical optics.

[29]  M. Griffith,et al.  Functional human corneal equivalents constructed from cell lines. , 1999, Science.

[30]  H. Sung,et al.  Biocompatibility study of a biological tissue fixed with a naturally occurring crosslinking reagent. , 1998, Journal of biomedical materials research.

[31]  A. Huc,et al.  Evaluation of different chemical methods for cros-linking collagen gel, films and sponges , 1996 .

[32]  T. J. T. P. Van Den Berg,et al.  Light transmittance of the human cornea from 320 to 700 nm for different ages , 1994, Vision Research.

[33]  A. Cornell-Bell,et al.  Expression of integrin and organization of F-actin in epithelial cells depends on the underlying surface. , 1994, Investigative ophthalmology & visual science.

[34]  J. Feijen,et al.  Relations between in vitro cytotoxicity and crosslinked dermal sheep collagens. , 1992, Journal of biomedical materials research.

[35]  A. Habeeb,et al.  Reaction of proteins with glutaraldehyde. , 1968, Archives of biochemistry and biophysics.

[36]  Lingli Li,et al.  A double network strategy to improve epithelization of a poly(2-hydroxyethyl methacrylate) hydrogel for corneal repair application , 2016 .

[37]  Yingjun Wang,et al.  Preparation and characterization of a novel tobramycin-containing antibacterial collagen film for corneal tissue engineering. , 2014, Acta biomaterialia.

[38]  M. M. Islam,et al.  The artificial cornea. , 2013, Methods in molecular biology.

[39]  Yingjun Wang,et al.  Crosslinked collagen-gelatin-hyaluronic acid biomimetic film for cornea tissue engineering applications. , 2013, Materials science & engineering. C, Materials for biological applications.

[40]  C. Connon,et al.  Corneal Regenerative Medicine , 2013, Methods in Molecular Biology.

[41]  W. Hodge,et al.  Collagen and glycopolymer based hydrogel for potential corneal application. , 2010, Acta biomaterialia.

[42]  M. Srinivasan,et al.  Corneal blindness: a global perspective. , 2001, Bulletin of the World Health Organization.

[43]  A. Jayakrishnan,et al.  Glutaraldehyde as a fixative in bioprostheses and drug delivery matrices. , 1996, Biomaterials.

[44]  M. Nimni,et al.  Mechanism of crosslinking of proteins by glutaraldehyde II. Reaction with monomeric and polymeric collagen. , 1982, Connective tissue research.